Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F50 I2 R1 209 44.2 1360995 98.7% 1343302 150.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,536,495 G→A A248V (GCG→GTG)  pdxK ← pyridoxal‑pyridoxamine kinase/hydroxymethylpyrimidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,536,4950GA100.0% 138.1 / NA 36A248V (GCG→GTG) pdxKpyridoxal‑pyridoxamine kinase/hydroxymethylpyrimidine kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/15);  total (21/15)

GAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTGCATCTCCTGATTTTCT  >  NC_000913/2536357‑2536635
                                                                                                                                          |                                                                                                                                            
gAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCa                                                                                                                                  <  1:632892/151‑1 (MQ=255)
 aaaaaTGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTc                                                                                                                                   >  1:214416/1‑149 (MQ=255)
    aaTGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCa                                                                                                                               >  2:325245/1‑150 (MQ=255)
      tGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGc                                                                                                                            >  2:86179/1‑151 (MQ=255)
           cccATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACtg                                                                                                                       >  2:513365/1‑151 (MQ=255)
                cGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACtgatga                                                                                                                   <  2:574482/150‑1 (MQ=255)
                cGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACtgatg                                                                                                                    >  2:61676/1‑149 (MQ=255)
                     ccATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGAtgagctgag                                                                                                             <  2:470645/151‑1 (MQ=255)
                       aTTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGAtgagctgagc                                                                                                            >  1:613039/1‑150 (MQ=255)
                          tttttATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGcaca                                                                                                         >  2:272956/1‑150 (MQ=255)
                          tttttATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGcaca                                                                                                         >  2:263778/1‑150 (MQ=255)
                             ttATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACaaaa                                                                                                      <  1:74284/150‑1 (MQ=255)
                                 gCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAgg                                                                                                  <  1:514831/150‑1 (MQ=255)
                                    tCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTc                                                                                                <  1:364208/149‑1 (MQ=255)
                                     ccGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGcc                                                                                             >  1:205380/1‑151 (MQ=255)
                                      cGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCa                                                                                            <  1:251068/151‑1 (MQ=255)
                                        ccAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGt                                                                                          >  2:278565/1‑151 (MQ=255)
                                                ggcAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTc                                                                                   >  1:6872/1‑150 (MQ=255)
                                                   aaaaTCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGt                                                                               <  2:135634/151‑1 (MQ=255)
                                                          aTTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGtttt                                                                        <  2:18282/151‑1 (MQ=255)
                                                             cATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTAc                                                                     >  2:359794/1‑151 (MQ=255)
                                                                cGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCg                                                                  <  1:492971/151‑1 (MQ=255)
                                                                 gCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGt                                                                 <  2:108476/151‑1 (MQ=255)
                                                                      cATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATg                                                            <  1:651368/151‑1 (MQ=255)
                                                                        tgctgcTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATggg                                                          >  2:59859/1‑151 (MQ=255)
                                                                               gggTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATg                                                     <  1:488703/149‑1 (MQ=255)
                                                                               gggTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGa                                                    >  1:347740/1‑150 (MQ=255)
                                                                                          aTCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGt                                        >  1:2860/1‑151 (MQ=255)
                                                                                             aCTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCgg                                     >  1:6115/1‑151 (MQ=255)
                                                                                                tCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGc                                    >  1:314809/1‑149 (MQ=255)
                                                                                                 ccAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGt                                 <  1:644054/151‑1 (MQ=255)
                                                                                                       cgcgCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGaccac                           >  1:125042/1‑151 (MQ=255)
                                                                                                             aCCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAAc                     <  1:626248/151‑1 (MQ=255)
                                                                                                                      ggTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTGCAtctc            >  1:627511/1‑151 (MQ=255)
                                                                                                                            cTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTGCATCTCCTGAtt      >  2:589194/1‑151 (MQ=255)
                                                                                                                                aTCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTGCATCTCCTGATTttct  >  1:637175/1‑151 (MQ=255)
                                                                                                                                          |                                                                                                                                            
GAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTGCATCTCCTGATTTTCT  >  NC_000913/2536357‑2536635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A