Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	781,084	T→C	noncoding (17/76 nt)	valZ →	tRNA‑Val

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	781,084	0	T	C	100.0%	136.9 / NA	43	noncoding (17/76 nt)	valZ	tRNA‑Val
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (23/20); total (23/20)

AAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTA  >  NC_000913/780943‑781228

aaGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGGATCCAGCCCAGTATCGGGTGATTAGCTCAGCCGGGAGAGc                                                                                                                                          >  1:188608/1‑150 (MQ=255)

 aGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGAGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCAc                                                                                                                                        >  1:490243/1‑151 (MQ=255)

   cGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCt                                                                                                                                      >  2:638937/1‑151 (MQ=255)

    gtgtAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTc                                                                                                                                     <  1:566289/151‑1 (MQ=255)

       tAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCt                                                                                                                                  >  2:470534/1‑151 (MQ=255)

            tAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACa                                                                                                                              >  2:272358/1‑150 (MQ=255)

             aaCGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGCTCGGAGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACa                                                                                                                              >  1:219108/1‑149 (MQ=255)

             aaCGTGGCGTGAGCAACGGCGCGTAGGGCGAGGGAAGCGAGTCATACTGCACGACCCACCACTAATGACGGTGGGTTCGGTCAAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACaa                                                                                                                             <  1:439263/150‑1 (MQ=37)

              aCGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAagg                                                                                                                           >  2:629365/1‑151 (MQ=255)

              aCGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAag                                                                                                                            >  2:162446/1‑150 (MQ=255)

               cGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAagga                                                                                                                          <  1:592716/151‑1 (MQ=255)

                gTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAaggag                                                                                                                         <  2:548231/151‑1 (MQ=255)

                gTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAaggag                                                                                                                         <  2:364965/151‑1 (MQ=255)

                    cGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAggggg                                                                                                                     >  2:60761/1‑151 (MQ=255)

                        aGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAAAAGTTTGTAGGATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGTGGGGGTcgg                                                                                                                 >  1:468346/1‑151 (MQ=255)

                           aaCGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTcggcgg                                                                                                              >  2:660140/1‑151 (MQ=255)

                                     aGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCg                                                                                                    <  1:60762/151‑1 (MQ=255)

                                      gggcgggcAAAGGGGGTCATCCTGAGGGACCCACAACTAAGGACGCGGGTTTCGATGAAAGTAGTTTGTGTTTTCAAGGGCAGTACGGGGTGATTAGCCCAGCCGGGAGA‑CCCCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATccc                                                                                                     <  1:478410/148‑1 (MQ=12)

                                                     gTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACt                                                                                     <  2:175566/150‑1 (MQ=255)

                                                                 gACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGc                                                                         >  2:551097/1‑150 (MQ=255)

                                                                 gACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCt                                                                        <  2:408147/151‑1 (MQ=255)

                                                                 gACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCt                                                                        <  2:244019/151‑1 (MQ=255)

                                                                   cccACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCa                                                                      <  1:469795/151‑1 (MQ=255)

                                                                    ccaccaCTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAg                                                                     <  2:297563/151‑1 (MQ=255)

                                                                         acTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGt                                                                >  1:206418/1‑151 (MQ=255)

                                                                          cTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCCGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTgg                                                                 <  1:151430/149‑1 (MQ=255)

                                                                               gACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCCCCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGc                                                           <  1:314719/150‑1 (MQ=255)

                                                                               gACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGc                                                           >  2:331479/1‑150 (MQ=255)

                                                                                  ggTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGGTGGTAGAGCAGtt                                                       >  1:374850/1‑151 (MQ=255)

                                                                                   gTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCCCCCTTTCCAGGAGGGGGCCGGCGGTTCGATTCCGTAAGCAACCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTg                                                      <  1:357531/151‑1 (MQ=17)

                                                                                      ggTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTg                                                      <  2:209593/148‑1 (MQ=255)

                                                                                       gTTCGGTTGCAGTAGTTTTTATTCTACAGCGCCGTCTCCGGTGATTATCTCAGCCGAGACATCCCCTCCTTCACACGGCGGGGCTCGGCGGTTCGATCCCGTCATCCCCCCCCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACt                                                   <  1:204502/150‑1 (MQ=9)

                                                                                          tggTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACtttt                                                >  1:250095/2‑150 (MQ=255)

                                                                                            gTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTaa                                              >  2:363688/1‑150 (MQ=255)

                                                                                                     gtTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGt                                     >  1:89513/1‑150 (MQ=38)

                                                                                                     gtTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTc                                    >  2:83320/1‑151 (MQ=37)

                                                                                                               tCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCg                           <  1:83319/150‑1 (MQ=25)

                                                                                                                ccAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGCTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGa                          >  1:535510/1‑150 (MQ=25)

                                                                                                                 cAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAAt                        <  2:653017/151‑1 (MQ=25)

                                                                                                                             gggTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGAccc            >  2:43874/1‑151 (MQ=18)

                                                                                                                             gggTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGAccc            >  2:176044/1‑151 (MQ=18)

                                                                                                                             gggTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGAccc            >  2:151584/1‑151 (MQ=18)

                                                                                                                                         cAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGCGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTa  <  1:293729/149‑1 (MQ=18)

AAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTA  >  NC_000913/780943‑781228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A