Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	781,084	T→C	noncoding (17/76 nt)	valZ →	tRNA‑Val

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	781,084	0	T	C	100.0%	98.8 / NA	32	noncoding (17/76 nt)	valZ	tRNA‑Val
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (17/15); total (17/15)

GATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTT  >  NC_000913/780937‑781226

gATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCggg                                                                                                                                             >  1:371990/1‑151 (MQ=255)

 aTGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGga                                                                                                                                            <  2:195761/151‑1 (MQ=255)

                   aaCGTTGCATCAGCAACGGCCCGTGGGGTATGCGACGCGAGTCATCCTGCACGACCCCCCTCTAAGTGCGGTGGGGACGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGGACCGCCCt                                                                                                                                <  2:618764/145‑1 (MQ=21)

                      gTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGACAGCACCTCCCTTACa                                                                                                                            >  1:194817/1‑146 (MQ=255)

                         gCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAgggg                                                                                                                    <  2:170216/151‑1 (MQ=255)

                                  aCGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACTACCCTCCACTAATGACGCTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGt                                                                                                           <  2:508994/151‑1 (MQ=255)

                                  aCGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGt                                                                                                           >  1:339675/1‑151 (MQ=255)

                                        catAGGGCGAGGCACGCGGGTCGTGATGAACGCCCCTCCACTAATGCCGGTGGCTTCGATGGTAGTAGGTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATc                                                                                                     <  2:121457/149‑1 (MQ=17)

                                                    gaagcgaGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATcaccc                                                                                         >  1:137857/1‑151 (MQ=255)

                                                              aTCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCggg                                                                               <  2:533236/151‑1 (MQ=255)

                                                                          ccaccaCTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTc                                                                     >  2:595709/1‑149 (MQ=255)

                                                                             ccacTAATGACGGTGGGTTCGGTGGAAGTTGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTg                                                                <  1:620859/151‑1 (MQ=255)

                                                                                     gACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCa                                                        <  1:321093/151‑1 (MQ=255)

                                                                                       cGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGt                                                      <  1:547332/151‑1 (MQ=255)

                                                                                       cGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGGAGAGCAg                                                       >  1:7664/1‑150 (MQ=255)

                                                                                                    ggAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGGAGTTGACTTTTAAt                                           >  2:23088/1‑149 (MQ=255)

                                                                                                        gtagtTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTg                                     >  1:183915/1‑151 (MQ=255)

                                                                                                           gtTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGt                                   <  1:87708/150‑1 (MQ=38)

                                                                                                            tttGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCg                                 >  1:148375/1‑151 (MQ=37)

                                                                                                             ttGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTgg                                    <  2:560937/147‑1 (MQ=25)

                                                                                                             ttGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGGTGATTTTTAATCAATTGGTCGc                                >  1:53164/1‑151 (MQ=255)

                                                                                                               gtagtaTCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAg                              <  1:145317/151‑1 (MQ=25)

                                                                                                                 agtaTCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGt                            >  2:341398/1‑151 (MQ=25)

                                                                                                                 agtaTCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGt                            <  2:148371/151‑1 (MQ=25)

                                                                                                                     tCCAGCGCAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCg                         >  2:177624/1‑150 (MQ=25)

                                                                                                                            cAGTATCGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGc                 <  1:514702/151‑1 (MQ=21)

                                                                                                                                  cGGGTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGAcc           >  2:516468/1‑151 (MQ=18)

                                                                                                                                   gggTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCCGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGAccc          >  1:574699/1‑151 (MQ=18)

                                                                                                                                   gggTGATTAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGAccc          >  2:507768/1‑151 (MQ=18)

                                                                                                                                         ttAGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGt    >  2:330743/1‑151 (MQ=18)

                                                                                                                                           aGCTCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGtt   <  2:183910/150‑1 (MQ=18)

                                                                                                                                              tCAGCCGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCCCGGTCGTTAGCTCAGGTGGTAGAGCAGATGACTTTTAATCAATTGGTTGCAGGGTCGAATCCTGCACGACCCACCAtttt  >  2:602286/1‑148 (MQ=11)

GATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCACTAATGACGGTGGGTTCGGTGGAAGTAGTTTGTAGTATCCAGCGCAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTT  >  NC_000913/780937‑781226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A