Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	835,945	A→G	Y233C (TAT→TGT)	ybiB →	putative family 3 glycosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	835,945	0	A	G	100.0%	99.6 / NA	32	Y233C (TAT→TGT)	ybiB	putative family 3 glycosyltransferase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (17/15); total (17/15)

CGAAACTGGCGACGCCATTTGCCGAAGGTGAGGCGCTGCGTCTTTCCAGCGTTTCGCATCCGGAATACATTGGACGCGTCGCGAAGTTCTTTAGCGATATCGGCGGGCGGGCGCTACTAATGCATGGTACTGAAGGTGAAGTGTATGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTT  >  NC_000913/835801‑836088

cGAAACTGGCGACGCCATTTGCCGAAGGTGAGGCGCTGCGTCTTTCCAGCGTTTCGCATCCGGAATACATTGGACGCGTCGCGAAGTTCTTTAGCGATATCGGCGGGCGGGCGCTACTAATGCATGGTACTGAAGGTGAAGTGTGTGCTaa                                                                                                                                           <  1:98543/151‑1 (MQ=255)

       ggCGACGCCATTTGCCGAAGGTGAGGCGCTGCGTCTTTCCAGCGTTTCGCATCCGGAATACATTGGACGCGTCGCGAAGTTCTTTAGCGATATCGGCGGGCGGGCGCTACTAATGCATGGTACTGAAGGTGAAGTGTGTGCTAATCCGCAg                                                                                                                                    <  1:181038/151‑1 (MQ=255)

        gCGACGCCATTTGCCGAAGGTGAGGCGCTGCGTCTTTCCAGCGTTTCGCATCCGGAATACATTGGACGCGTCGCGAAGTTCTTTAGCGATATCGGCGGGCGGGCGCTACTAATGCATGGTACTGAAGGTGAAGTGTGTGCTAATCCGCAgc                                                                                                                                   <  2:410248/151‑1 (MQ=255)

             gCCATTTGCCGAAGGTGAGGCGCTGCGTCTTTCCAGCGTTTCGCATCCGGAATACATTGGACGCGTCGCGAAGTTCTTTAGCGATATCGGCGGGCGGGCGCTACTAATGCATGGTACTGAAGGTGAAGTGTGTGCTAATCCGCAGCGCTGc                                                                                                                              <  2:554767/151‑1 (MQ=255)

                 tttGCCGAAGGTGAGGCGCTGCGTCTTTCCAGCGTTTCGCATCCGGAATACATTGGACGCGTCGCGAAGTTCTTTAGCGATATCGGCGGGGGGGCGCTACTAATGCATGGGACTGAAGGGGAAGTGGGTGGTAATCCGCAGCGCTGccc                                                                                                                            >  2:377908/1‑149 (MQ=255)

                           gTGAGGCGCTGCGTCTTTCCAGCGTTTCGCATCCGGAATACATTGGACGCGTCGCGAAGTTCTTTAGCGATATCGGCGGGCGGGGGCTACTTATGCATGGTACTGAAGGTGGAGTGGGGGCTAATCCGCAGCGccgcccgccaat                                                                                                                      >  2:109283/1‑141 (MQ=255)

                                  gcTGCGTCTTTCCAGCGTTTCGCATCCGGAATACATTGGACGCGTCGCGAAGTTCTTTAGCGATATCGGCGGGCGGGCGCTACTAATGCATGGTACTGAAGGTGAAGTGTGTGCTAATCCGCAGCGCt                                                                                                                                <  2:133510/128‑1 (MQ=255)

                                    tGCGTCTTTCCAGCGTTTCGCATCCGGAATACATTGGACGCGTCGCGAAGTTCTTTAGCGATATCGGCGGGCGGGGGCTACTAATGGCTGGGACTTTAGGGGGAGGGGGGGGCAATCCGCAGCGCCGCCCGCAGATCAATTTCCtttaac                                                                                                        >  1:154650/1‑146 (MQ=255)

                                      cGTCTTTCCAGCGTTTCGCATCCGGAATACATTGGACGCGTCGCGAAGTTCTTTAGCGATATCGGCGGGCGGGCGCTACTAATGCATGGTACTGAAGGTGAAGTGTGTGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTg                                                                                                     <  1:234978/151‑1 (MQ=255)

                                           ttCCAGCGTTTCGCATCCGGAATACATTGGACGCGTCGCGAAGTTCTTTAGCGATATCGGCGGGCGGGCGCTACTAATGCATGGTACTGAAGGTGAAGTGTGTGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAg                                                                                                  <  2:86549/149‑1 (MQ=255)

                                                   tttCGCATCCGGAATACATTGGACGCGTCGCGAAGTTCTTTAGCGATATCGGCGGGCGGGCGCTACTAATGCATGGTAATTAAGGGGGAGGGTGTGCTAATCCGCAGCGCTGCCCGCAGATCAATATCATTTACCGGGGAGGGGTGCgggg                                                                                        >  2:330680/1‑150 (MQ=255)

                                                    ttCGCATCCGGAATACATTGGACGCGTCGCGAAGTTCTTTAGCGATATCGGCGGGCGGGCGCTACTAATGCATGGTACTGAAGGTGAAGTGTGTGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGt                                                                                        <  1:570199/150‑1 (MQ=255)

                                                          tCCGGAATACATTGGACGCGTCGCGAAGTTCTTTAGCGATATCGGCGGGCGGGCGCTACTAATGCATGGTACTGAAAGGGAAGTGTGTGCTAATCCGCAGCGCTGCCCGCAGAACAATCTCATTGACCGGGGAGGGAAGGGGGGGCTGt                                                                                   >  2:187194/1‑149 (MQ=255)

                                                                aTACATTGGACGCGTCGCGAAGTTCTTTAGCGATATCGGCGGGCGGGCGCTACTAATTCATGGTAATGAAGGGGAAGGGTGTGGTAATTCCCCGCCCTGCCCCCAGAACAATCTCATTTACCGTGAAGGGATGGGGGGGCTGTATGaaaa                                                                            >  2:546030/1‑150 (MQ=255)

                                                                     ttGGACGCGTCGCGAAGTTCTTTAGCGATATCGGCGGGCGGGCGCTACTAATGCATGGTACTGAAGGGGGAGTGGGTGCTAATCCGCAGCGCTGCCCCCAGATCAAACTCATTTACCGTGAAGGGGTGCGGGGGCTGTATGaaaaaaa                                                                         >  2:393578/1‑146 (MQ=255)

                                                                               cgcgAAGTTCTTTAGCGATATCGGCGGGCGGGGGGCACCTATAGCGGGGAAGGAAAGGGAAGAGGGTGGGGAAGCCCCGCGCTGCCCGCAGATCAATTTCATTGACCGGGAAGGGGTGGGGGGGCTGGAATaaaaaaaaggcacaaccg                                                              >  1:235500/1‑136 (MQ=255)

                                                                               cgcgAAGTTCTTTAGCGATATCGGCGGGCGGGGGCTAATAATGCATGGGACTGAAGGGGAAGTGTGTGGTAAAACGCAGGGCTGCCCGCAGATCAATTTCATTGACCGGGAAGGGATGGGGGGGGGGTATGAAAAAAAGGACACTGCTgg                                                             >  2:670148/1‑150 (MQ=255)

                                                                                  gAAGTTCTTTAGCGATATCGGCGGGCGGGCGCTACTAATGCATGGTACTGAAGGTGAAGTGTGTGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGc                                                         <  2:94672/151‑1 (MQ=255)

                                                                                   aaGTTCTTTAGCGATATCGGCGGGCGGGCGCTAATTATACATGGTACCGAAGGCGAAGTGGGGGCTCATCCGCAGCGCTGCCCCCAGAGCAATCTCATTGAACGTGAAGGGGTGCGGGTGGTGTATGAAAAAAAAGAAActtc                                                                >  2:93887/1‑141 (MQ=255)

                                                                                                  atCGGCGGGCGGGCGCTACTAATGCATGGTACTGAAGGTGAAGTGTGTGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAg                                         <  1:273872/151‑1 (MQ=255)

                                                                                                   tCGGCGGGCGGGCGCTACTAATGCATGGTACTGGAGGTGAAGTGTGTGCTAATCCGCCGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGGGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAg                                         >  1:415583/1‑150 (MQ=255)

                                                                                                         ggcgggcgCTACTAATGCATGGTACTGAAGGTGAAGTGTGTGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGa                                  <  1:380051/151‑1 (MQ=255)

                                                                                                             ggcgCTACTAATGCATGGTACTGAAGGTGAAGTGTGTGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCg                              <  1:640128/151‑1 (MQ=255)

                                                                                                                      aaTGCATGGTACTGAAGGTGAAGTGTGTGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCa                       <  2:333382/149‑1 (MQ=255)

                                                                                                                      aaTGCATGGTACTGAAGGTGAAGTGTGTGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACg                     <  1:378988/151‑1 (MQ=255)

                                                                                                                      aaTGCATGGTACTGAAGGTGAAGTGGGTGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACg                     >  1:325020/1‑151 (MQ=255)

                                                                                                                        tGCATGGTACTGAAGGTGAAGTGTGTGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGgc                   >  2:643649/1‑151 (MQ=255)

                                                                                                                             ggTACTGAAGGTGAAGTGTGTGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGt              <  2:223802/151‑1 (MQ=255)

                                                                                                                                  tGAAGGTGAAGTGTGTGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGa           >  1:9931/1‑149 (MQ=255)

                                                                                                                                  tGAAGGTGAAGTGTGTGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGAtt         >  2:13513/1‑151 (MQ=255)

                                                                                                                                        tGAAGTGTGTGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGt   >  2:551290/1‑151 (MQ=255)

                                                                                                                                         gAAGTGTGTGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGtt  >  1:657927/1‑151 (MQ=255)

CGAAACTGGCGACGCCATTTGCCGAAGGTGAGGCGCTGCGTCTTTCCAGCGTTTCGCATCCGGAATACATTGGACGCGTCGCGAAGTTCTTTAGCGATATCGGCGGGCGGGCGCTACTAATGCATGGTACTGAAGGTGAAGTGTATGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTT  >  NC_000913/835801‑836088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A