Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,241,779	A→G	L98P (CTG→CCG)	cvrA ←	putative cation/proton antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,241,779	0	A	G	100.0%	145.0 / NA	41	L98P (CTG→CCG)	cvrA	putative cation/proton antiporter
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (26/15); total (26/15)

TTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAACGGGATACCGCCGACG  >  NC_000913/1241641‑1241925

ttACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCg                                                                                                                                        >  1:518224/1‑151 (MQ=255)

 tACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGc                                                                                                                                         <  1:434442/149‑1 (MQ=255)

  accaccCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGAc                                                                                                                                      >  2:134058/1‑151 (MQ=255)

                      ccGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCcac                                                                                                                  >  2:429542/1‑151 (MQ=255)

                      ccGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCcac                                                                                                                  >  2:135560/1‑151 (MQ=255)

                         cTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACg                                                                                                               >  2:330866/1‑151 (MQ=255)

                          tGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACg                                                                                                               >  2:320883/1‑150 (MQ=255)

                                        agCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTg                                                                                                >  2:65085/1‑151 (MQ=255)

                                            gacgaTAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTg                                                                                            >  1:555548/1‑151 (MQ=255)

                                            gacgaTAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTg                                                                                            >  2:165677/1‑151 (MQ=255)

                                              cgaTAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGt                                                                                             >  1:454174/1‑148 (MQ=255)

                                              cgaTAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTgcg                                                                                          >  1:527864/1‑151 (MQ=255)

                                                  aGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCAt                                                                                       <  2:143007/150‑1 (MQ=255)

                                                       cGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCccgcc                                                                                 >  1:102666/1‑151 (MQ=255)

                                                           taataaGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTc                                                                              <  2:527100/150‑1 (MQ=255)

                                                           taataaGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCg                                                                             >  2:489643/1‑151 (MQ=255)

                                                            aataaGCCTTCAACCAAATCAAAATTAAACATCCACGCCGCCATCATGCCGGTTAAGCCAGATGTGATAAGCTCGCCCGGCGGCGCCGTCGACAGTCCCTTTCCTAACGCCACACGAAAGGAATTGGCTGGAGTGCGCATCTCGCCGTCgt                                                                            <  1:204574/151‑2 (MQ=255)

                                                             ataaGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAg                                                                           <  1:479004/151‑1 (MQ=255)

                                                               aaGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGaa                                                                                                        <  2:22456/120‑1 (MQ=255)

                                                               aaGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGaa                                                                                                        >  1:22457/1‑120 (MQ=255)

                                                               aaGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAg                                                                           >  1:15960/1‑149 (MQ=255)

                                                                     tcaatcaaATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAaata                                                                    >  2:562470/1‑150 (MQ=255)

                                                                       aatcaaatcaAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATACCGCCGTCGAGCAAAATAATc                                                                 >  1:492870/1‑151 (MQ=255)

                                                                                  gATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAg                                                      <  2:638481/151‑1 (MQ=255)

                                                                                   aTTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAg                                                      <  1:429537/150‑1 (MQ=255)

                                                                                      aaaCAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGtt                                                   >  2:233791/1‑150 (MQ=255)

                                                                                         cAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGttactt                                               >  1:441196/1‑151 (MQ=255)

                                                                                           gCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGttacttac                                             <  1:261877/151‑1 (MQ=255)

                                                                                              aCGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCAt                                          <  2:322130/151‑1 (MQ=255)

                                                                                                gccgccATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGt                                        >  2:164904/1‑151 (MQ=255)

                                                                                                     catcatGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCg                                   >  2:251998/1‑151 (MQ=255)

                                                                                                            ccGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAgggg                             >  1:234608/1‑150 (MQ=255)

                                                                                                            ccGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGt                            <  1:134054/151‑1 (MQ=255)

                                                                                                               gTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAAt                         <  2:102676/151‑1 (MQ=255)

                                                                                                                         agGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAACgg               <  2:102669/151‑1 (MQ=255)

                                                                                                                         agGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAACgg               <  1:419351/151‑1 (MQ=255)

                                                                                                                                aaGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAGCGGGATAccg        >  2:86079/1‑151 (MQ=255)

                                                                                                                                  gCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAACGGGATAccgcc      <  1:575558/151‑1 (MQ=255)

                                                                                                                                    aCGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAACGGGATAccgccg     >  2:401638/1‑150 (MQ=255)

                                                                                                                                      gCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAACGGGATACCGCCGAc   >  2:651172/1‑150 (MQ=255)

                                                                                                                                      gCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAACGGGATACCGCCGACg  >  2:160984/1‑151 (MQ=255)

TTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAACGGGATACCGCCGACG  >  NC_000913/1241641‑1241925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A