Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,541,602	T→C	Y330C (TAC→TGC)	narZ ←	nitrate reductase 2 (NRZ), alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,541,602	0	T	C	100.0%	103.9 / NA	32	Y330C (TAC→TGC)	narZ	nitrate reductase 2 (NRZ), alpha subunit
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (16/16); total (16/16)

ACTGCTACGGTTTTCCACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGAGTAGTCAGGGGTA  >  NC_000913/1541455‑1541748

aCTGCTACGGTTTTCCACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCagcagt                                                                                                                                                 <  2:502730/151‑1 (MQ=255)

   gCTACGGTTTTCCACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGtt                                                                                                                                                <  1:453203/149‑1 (MQ=255)

      aCGGTTTTCCACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATaa                                                                                                                                           <  2:324409/151‑1 (MQ=255)

      aCGGTTTTCCACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATa                                                                                                                                            <  1:578022/150‑1 (MQ=255)

         gTTTTCCACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATaaa                                                                                                                                          >  1:117475/1‑149 (MQ=255)

         gTTTTCCACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATAAag                                                                                                                                         >  1:26867/1‑150 (MQ=255)

           tttCCACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCCGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCGGTTGATaaa                                                                                                                                          >  1:75290/1‑147 (MQ=255)

              ccACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATAAAGTAGTc                                                                                                                                    <  2:66048/150‑1 (MQ=255)

              ccACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATAAAGTAGTc                                                                                                                                    <  2:273389/150‑1 (MQ=255)

              ccACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATAAAGTAGTCg                                                                                                                                   <  1:546854/151‑1 (MQ=255)

               cACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATAAAGTAGTCGc                                                                                                                                  >  1:397180/1‑151 (MQ=255)

                     ggATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCCGTTGGTAAAGGAGTCGATggg                                                                                                                              >  1:325846/1‑149 (MQ=255)

                          gttgCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATAAAGTAGTCGCTGGGATTATCg                                                                                                                       <  1:559838/151‑1 (MQ=255)

                          gttgCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATAAAGTAGTCGCTGGGATTATCg                                                                                                                       <  1:603625/151‑1 (MQ=255)

                           ttgCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATAAAGTAGTCGCTGGGATTATc                                                                                                                        <  2:453604/149‑1 (MQ=255)

                                       aGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCtt                                                                                                          >  2:134084/1‑151 (MQ=255)

                                          ccatccaCCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTa                                                                                                       <  1:122640/151‑1 (MQ=255)

                                              ccaccaGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAAt                                                                                                   >  2:667388/1‑151 (MQ=255)

                                                accaGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATc                                                                                                  >  2:618975/1‑150 (MQ=255)

                                                   aGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGGTAAAGGAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACAt                                                                                              >  1:394204/1‑151 (MQ=255)

                                                              gcgGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGcc                                                                                   >  1:633853/1‑151 (MQ=255)

                                                                 gATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATc                                                                                <  2:492294/151‑1 (MQ=255)

                                                                             gggAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATAGCCCGGgcgc                                                                      >  1:669130/1‑149 (MQ=255)

                                                                                   gTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATAAAGAAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGt                                                               >  2:288023/1‑150 (MQ=255)

                                                                                      gCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCt                                                           >  2:212341/1‑151 (MQ=255)

                                                                                            aTCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTc                                                     <  1:608116/151‑1 (MQ=255)

                                                                                                 tgcgAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCCGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTg                                                 >  1:148799/2‑150 (MQ=255)

                                                                                                     aGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTgccagc                                            >  1:506499/1‑151 (MQ=255)

                                                                                                          ccAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTg                                       >  1:204284/1‑151 (MQ=255)

                                                                                                                                   gCTGTAGCGGCGGCAGCAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCagag              <  1:68996/151‑1 (MQ=255)

                                                                                                                                      gTAGCGGCGGCAGCAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGagta            <  2:524010/150‑1 (MQ=255)

                                                                                                                                          cggcggCAGCAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGAGTAGTCAg       <  1:490876/151‑1 (MQ=255)

                                                                                                                                              ggcAGCAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGAGTAGTCAGGGGt   <  2:633863/151‑1 (MQ=255)

                                                                                                                                               gcagcaGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGAGTAGTCAGGGGTa  <  2:371900/151‑1 (MQ=255)

                                                                                                                                               gcagcaGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGAGTAGTCAGGGGTa  <  2:512961/151‑1 (MQ=255)

ACTGCTACGGTTTTCCACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGAGTAGTCAGGGGTA  >  NC_000913/1541455‑1541748

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A