Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,967,290	C→T	G50S (GGT→AGT)	cheY ←	chemotaxis regulator transmitting signal to flagellar motor component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,967,290	0	C	T	100.0%	164.8 / NA	44	G50S (GGT→AGT)	cheY	chemotaxis regulator transmitting signal to flagellar motor component
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (17/27); total (17/27)

GCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACCGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAGTCATCCACAACCAAAAATTTAAGTTCTTTATCCGCCAT  >  NC_000913/1967142‑1967437

gccgcAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTgc                                                                                                                                                   <  1:275802/151‑1 (MQ=255)

 ccgcAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTgcc                                                                                                                                                  <  2:145375/151‑1 (MQ=255)

         atgatgTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAActgcctgcctgc                                                                                                                                           >  1:24718/1‑150 (MQ=255)

         atgatgTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCa                                                                                                                                          <  2:362118/151‑1 (MQ=255)

                     ttcttcGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTgaga                                                                                                                               <  2:191049/150‑1 (MQ=255)

                     ttcttcGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTga                                                                                                                                 <  1:583609/148‑1 (MQ=255)

                     ttcttcGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTga                                                                                                                                 <  2:499972/148‑1 (MQ=255)

                         tcGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCg                                                                                                                            <  1:507619/149‑1 (MQ=255)

                         tcGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGt                                                                                                                           <  1:332546/150‑1 (MQ=255)

                          cGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTc                                                                                                                          <  1:559298/150‑1 (MQ=255)

                           gCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCg                                                                                                                         >  2:287576/1‑150 (MQ=255)

                             ttCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGAc                                                                                                                       >  1:377196/1‑150 (MQ=255)

                              tCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACg                                                                                                                      <  2:371624/150‑1 (MQ=255)

                                tGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGcc                                                                                                                    >  1:237570/1‑150 (MQ=255)

                                tGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGcc                                                                                                                    >  2:168573/1‑150 (MQ=255)

                                 gCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCa                                                                                                                   <  2:93493/150‑1 (MQ=255)

                                  cAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCGGCCTGCAACTTATTGAGAGCGTCGACGCCATc                                                                                                                 <  1:28972/151‑1 (MQ=255)

                                      cACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTcc                                                                                                             <  1:459824/151‑1 (MQ=255)

                                                   ggCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACat                                                                                                >  2:73293/1‑151 (MQ=255)

                                                   ggCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACa                                                                                                 <  1:230393/150‑1 (MQ=255)

                                                                    gcCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGctct                                                                               >  1:72696/1‑151 (MQ=255)

                                                                      cATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCttt                                                                             >  1:66724/1‑151 (MQ=255)

                                                                           gCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTcagca                                                                        <  2:236910/151‑1 (MQ=255)

                                                                             aCGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAgg                                                                      >  2:533583/1‑151 (MQ=255)

                                                                               gAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGtt                                                                    >  1:445561/1‑151 (MQ=255)

                                                                                    gTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGc                                                                <  1:666849/150‑1 (MQ=255)

                                                                                           gCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCg                                                        <  1:473639/151‑1 (MQ=255)

                                                                                            cAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGt                                                       <  2:666078/151‑1 (MQ=255)

                                                                                             aaTTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTc                                                      >  2:24636/1‑151 (MQ=255)

                                                                                                   aGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCAtgg                                                >  1:87134/1‑151 (MQ=255)

                                                                                                      ccatccatATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCAtggtgg                                             >  2:643286/1‑151 (MQ=255)

                                                                                                         tccatATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGaa                                           >  2:397186/1‑150 (MQ=255)

                                                                                                                 gggCATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAGTCATcc                                  <  2:24717/151‑1 (MQ=255)

                                                                                                                    cATGTTCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAGTCATCcaca                               <  1:299408/151‑1 (MQ=255)

                                                                                                                        ttCCAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAGTCATCCACAAcc                            >  1:401885/1‑150 (MQ=255)

                                                                                                                          ccAGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAGTCATCCACAACCaa                          <  2:435769/150‑1 (MQ=255)

                                                                                                                            aGTCGGAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAGTCATCCACAACCaaaaa                       <  2:56221/151‑1 (MQ=255)

                                                                                                                                ggAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAGTCATCCACAACCAAAAATTTa                   <  2:445566/151‑1 (MQ=255)

                                                                                                                                ggAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAGTCATCCACAACCAAAAATTTa                   <  1:643276/151‑1 (MQ=255)

                                                                                                                                ggAGATAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAGTCATCCACAACCAAAAATTTa                   <  1:24637/151‑1 (MQ=255)

                                                                                                                                  agaTAACAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAGTCATCCACAACCAAAAATTTa                   >  2:514132/1‑149 (MQ=255)

                                                                                                                                      aaCAAATCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAGTCATCCACAACCAAAAATTTAAGTTCt             >  1:178289/1‑151 (MQ=255)

                                                                                                                                          aaTCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAGTCATCCACAACCAAAAATTTAAGTTCTTTAt         <  1:513881/151‑1 (MQ=255)

                                                                                                                                           aTCCATAACTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAGTCATCCACAACCAAAAATTTAAGTTCTTTATc        <  2:592781/151‑1 (MQ=255)

                                                                                                                                                 aaCTGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAGTCATCCACAACCAAAAATTTAAGTTCTTTATCCGCCAt  >  2:73354/1‑151 (MQ=255)

GCCGCAGCAATGATGTTCTCTTTCTTCGCTTCTGCAGTCACCATTAACACTGGCAATGCCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACCGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTATTGAATCCCAGCTCTTTCAGCAGGTTACGCACTATGCGTCGCATGGTGGAAAAGTCATCCACAACCAAAAATTTAAGTTCTTTATCCGCCAT  >  NC_000913/1967142‑1967437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A