Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,974,562	T→C	T255A (ACG→GCG)	cheA ←	fused chemotactic sensory histidine kinase in two‑component regulatory system with CheB and CheY: sensory histidine kinase/signal sensing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,974,562	0	T	C	100.0%	124.5 / NA	36	T255A (ACG→GCG)	cheA	fused chemotactic sensory histidine kinase in two‑component regulatory system with CheB and CheY: sensory histidine kinase/signal sensing protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (19/17); total (19/17)

CATGATTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGTCATC  >  NC_000913/1974424‑1974709

cATGATTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTc                                                                                                                                         >  2:188512/1‑151 (MQ=255)

      tAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCAcgcg                                                                                                                                   >  1:574662/1‑151 (MQ=255)

         ccGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGcc                                                                                                                                <  2:224594/151‑1 (MQ=255)

                         ggAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTc                                                                                                                 <  1:501331/150‑1 (MQ=255)

                           aaCGATGGGCACGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGGGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGc                                                                                                              >  1:529182/1‑151 (MQ=255)

                                  ggCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGt                                                                                                       <  2:456310/151‑1 (MQ=255)

                                   gCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGtt                                                                                                      <  2:511133/151‑1 (MQ=255)

                                           ggACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACt                                                                                              <  2:610805/151‑1 (MQ=255)

                                            gACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACtg                                                                                             <  2:624164/151‑1 (MQ=255)

                                            gACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACtg                                                                                             <  2:515930/151‑1 (MQ=255)

                                                gggTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGTTGGATTCATTGCTACGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACtggt                                                                                           <  1:11815/149‑1 (MQ=255)

                                                gggTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACtggtgg                                                                                         <  2:517281/151‑1 (MQ=255)

                                                                           taattaatTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTAc                                                              >  1:59527/1‑151 (MQ=255)

                                                                              ttaattGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGt                                                           >  1:26311/1‑151 (MQ=255)

                                                                                  ttGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTc                                                        >  2:382401/1‑150 (MQ=255)

                                                                                   tGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCa                                                       >  2:199072/1‑150 (MQ=255)

                                                                                      tCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTATTGTTTCAAAgg                                                   >  2:246223/1‑151 (MQ=255)

                                                                                       cAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAgg                                                   <  2:589883/150‑1 (MQ=255)

                                                                                        aaCCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTa                                                 <  2:458135/151‑1 (MQ=255)

                                                                                        aaCCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTa                                                 <  1:551384/151‑1 (MQ=255)

                                                                                         aCCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTa                                                 >  2:260593/1‑150 (MQ=255)

                                                                                          ccTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAAt                                               <  2:71040/151‑1 (MQ=255)

                                                                                               tCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGAt                                          >  1:489529/1‑151 (MQ=255)

                                                                                                   ccGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGACGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGc                                      >  1:70994/1‑151 (MQ=255)

                                                                                                    cGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCt                                     >  1:51913/1‑151 (MQ=255)

                                                                                                    cGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGCGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCt                                     >  1:377631/1‑151 (MQ=255)

                                                                                                     gCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCtt                                    <  1:450649/151‑1 (MQ=255)

                                                                                                      cTACACGGATGCTGGCGGATTCGGTACTGCGCGTCGCATTTTCCCTCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGGGGGGTGGGTATTTTTGGCGAGACTTCTACTGTTTCAAAGGCAATCTGTTCGGCTTc                                   >  1:445573/1‑151 (MQ=255)

                                                                                                        acacGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCa                                  <  1:193262/150‑1 (MQ=255)

                                                                                                          acGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATc                               >  1:6066/1‑151 (MQ=255)

                                                                                                           cGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAAt                                >  1:550529/1‑149 (MQ=255)

                                                                                                             gATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATcac                             >  1:635644/1‑150 (MQ=255)

                                                                                                                         ttCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTAcc                >  2:236174/1‑151 (MQ=255)

                                                                                                                            aTTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCt             <  1:524983/151‑1 (MQ=255)

                                                                                                                                ctgcGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCtgtg          >  2:241639/1‑150 (MQ=255)

                                                                                                                                   cgcgTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGt      <  1:176861/151‑1 (MQ=255)

                                                                                                                                      gtcgtTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGTCAt   >  2:334448/1‑151 (MQ=255)

                                                                                                                                       tcgcTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGTCATc  >  1:641047/1‑151 (MQ=255)

CATGATTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGTCATC  >  NC_000913/1974424‑1974709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A