Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,036,629	A→G	A265A (GCA→GCG)	hchA →	glyoxalase III and Hsp31 molecular chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,036,629	0	A	G	100.0%	134.2 / NA	39	A265A (GCA→GCG)	hchA	glyoxalase III and Hsp31 molecular chaperone
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (20/19); total (20/19)

AAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCAGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGTAAGCGCATAATCAATCGCGGCCGCGTTAGTACTCACGACGTGGCTGCGTTTTTTCACGGTTAATTTAT  >  NC_000913/2036481‑2036774

aaacGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGc                                                                                                                                                 <  2:489981/151‑1 (MQ=255)

    gCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAAt                                                                                                                                             <  1:265227/151‑1 (MQ=255)

         agaTTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGtt                                                                                                                                        <  1:112107/151‑1 (MQ=255)

          gaTTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTg                                                                                                                                       <  2:64559/151‑1 (MQ=255)

               gCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTa                                                                                                                                   >  2:427068/1‑150 (MQ=255)

                cTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTaa                                                                                                                                  >  2:249578/1‑150 (MQ=255)

                   tatGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTg                                                                                                                              >  2:286673/1‑151 (MQ=255)

                    atGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTgg                                                                                                                             >  2:669177/1‑151 (MQ=255)

                            tCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGgcgc                                                                                                                       >  1:135581/1‑149 (MQ=255)

                                   aCCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGc                                                                                                              >  1:255113/1‑151 (MQ=255)

                                     cTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCt                                                                                                             >  1:26272/1‑150 (MQ=255)

                                       ggTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTg                                                                                                            <  2:29650/149‑1 (MQ=255)

                                             tCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCtt                                                                                                     <  2:607653/150‑1 (MQ=255)

                                              cGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTAc                                                                                                   <  2:318102/151‑1 (MQ=255)

                                                  gaagaaCTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCgg                                                                                               >  2:75669/1‑151 (MQ=255)

                                                  gaagaaCTGAAGAAAATGGGCATGAATATCAGTAATGACGACATCGCCGGGCGAATACATAAGGACCGTAAACTTCTCACCGGCGACGGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTAcgcg                                                                                                <  1:18567/150‑1 (MQ=255)

                                                      aaCTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTa                                                                                           <  2:401057/151‑1 (MQ=255)

                                                                 aTGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTAc                                                                                >  1:308666/1‑151 (MQ=255)

                                                                        tGAATATCATTAATGACGACATCCCCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGttt                                                                            >  1:529663/1‑148 (MQ=255)

                                                                           atatCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGt                                                                      >  1:383858/1‑151 (MQ=255)

                                                                                          acATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGTAAGCGCATaatcaat                                                       <  2:104780/151‑1 (MQ=255)

                                                                                                  gggCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGTAAGCGCATAATCAATCGCGGCcg                                               >  1:644565/1‑151 (MQ=255)

                                                                                                      gAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGTAAGCGCATAATCAATCGCTGCCGCGtt                                           >  2:125910/1‑151 (MQ=255)

                                                                                                         tACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGTAAGCGCATAATCAATCGCGGCCGCGTTAGt                                        >  1:458749/1‑151 (MQ=255)

                                                                                                          aCATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGTAAGCGCATAATCAATCGCGGCCGCGTTAGTa                                       <  1:112348/151‑1 (MQ=255)

                                                                                                              aaGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGTAAGCGCATAATCAATCGCGGCCGCGTTAGTACTCa                                   <  2:176212/151‑1 (MQ=255)

                                                                                                                 gACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGTAAGCGCATAATCAATCGCGGCCGCGTTAGTACTCacg                                 >  1:610458/1‑150 (MQ=255)

                                                                                                                   ccGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGTAAGCGCATAATCAATCGCGGCCGCGTTAGTACTCacgac                               <  2:376095/150‑1 (MQ=255)

                                                                                                                     gTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGTAAGCGCATAATCAATCGCGGCCGCGTTAGTACTCACGACGTg                            <  1:255051/151‑1 (MQ=255)

                                                                                                                       aaaCTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGTAAGCGCATAATCAATCGCGGCCGCGTTAGTACTCACGACGTgg                           <  2:520767/150‑1 (MQ=255)

                                                                                                                          cTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGTAAGCGCATAATCAATCGCGGCCGCGTTAGTACTCACGACGTGGCTGc                       >  2:500279/1‑151 (MQ=255)

                                                                                                                                   ggCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGTAAGCGCATAATCAATCGCGGCCGCGTTAGTACTCACGACGTGGCTGCGTTTTTTc               >  2:153435/1‑150 (MQ=255)

                                                                                                                                   ggCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGTAAGCGCATAATCAATCGCGGCCGCGTTAGTACTCACGACGTGGCTGCGTTTTTTCa              <  2:39515/151‑1 (MQ=255)

                                                                                                                                      gACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGTAAGCGCATAATCAATCGCGGCCGCGTTAGTACTCACGACGTGGCTGCGTTTTTTCACgg           <  2:60459/151‑1 (MQ=255)

                                                                                                                                         aGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGTAAGCGCATAATCAATCGCGGCCGCGTTAGTACTCACGACGTGGCTGCGTTTTTTCACGGtt         >  1:395395/1‑150 (MQ=255)

                                                                                                                                            ccTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGTAAGCGCATAATCAATCGCGGCCGCGTTAGTACTCACGACGTGGCTGCGTTTTTTCACGGTTAAt      <  2:631504/150‑1 (MQ=255)

                                                                                                                                             cTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGTAAGCGCATAATCAATCGCGGCCGCGTTAGTACTCACGACGTGGCTGCGTTTTTTCACGGTTAAttt    >  2:334150/1‑151 (MQ=255)

                                                                                                                                               tttGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGTAAGCGCATAATCAATCGCGGCCGCGTTAGTACTCACGACGTGGCTGCGTTTTTTCACGGTTAATTTa   <  1:266311/150‑1 (MQ=255)

                                                                                                                                               tttGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGTAAGCGCATAATCAATCGCGGCCGCGTTAGTACTCACGACGTGGCTGCGTTTTTTCACGGTTAATTTAt  >  2:202151/1‑151 (MQ=255)

AAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCAGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGTAAGCGCATAATCAATCGCGGCCGCGTTAGTACTCACGACGTGGCTGCGTTTTTTCACGGTTAATTTAT  >  NC_000913/2036481‑2036774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A