Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,177,605	T→C	G320G (GGA→GGG)	fbaB ←	fructose‑bisphosphate aldolase class I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,177,605	0	T	C	100.0%	131.8 / NA	37	G320G (GGA→GGG)	fbaB	fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (20/17); total (20/17)

CCTTATCCGTCCTACGGGGTGGTGTTGTTCGTATAAGGCGTATCAGGCGATAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGTCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTATCAACCCAGCCCGACCCATATAGCAGTTAGCTAACTG  >  NC_000913/2177470‑2177742

ccTTATCCGTCCTACGGGGTGGTGTTGTTCGTATAAGGCGTATCAGGCGATAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGcccc                                                                                                                            <  2:113834/151‑1 (MQ=255)

        gTCCTACGGGGTGGTGTTGTTCGTATAAGGCGTATCAGGCGATAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGcc                                                                                                                    >  2:540343/1‑151 (MQ=255)

         tCCTACGGGGTGGTGTTGTTCGTATAAGGCGTATCAGGCGATAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCt                                                                                                                   >  1:404399/1‑151 (MQ=255)

          ccTACGGGGTGGTGTTGTTCGTATAAGGCGTATCAGGCGATAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTg                                                                                                                  <  2:642334/151‑1 (MQ=255)

          ccTACGGGGTGGTGTTGTTCGTATAAGGCGTATCAGGCGATAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTg                                                                                                                  <  2:128390/151‑1 (MQ=255)

             aCGGGGTGGTGTTGTTCGTATAAGGCGTATCAGGCGATAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTgcgc                                                                                                               >  2:292222/1‑151 (MQ=255)

                  gtggtgTTGTTCGTATAAGGCGTATCAGGCGATAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTttg                                                                                                          <  2:2152/151‑1 (MQ=255)

                     gtgtTGTTCGTATAAGGCGTATCAGGCGATAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTttgttg                                                                                                       <  1:632356/151‑1 (MQ=255)

                      tgttgtTCGTATAAGGCGTATCAGGCGATAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGcgcg                                                                                                              <  2:42838/143‑1 (MQ=255)

                      tgttgtTCGTATAAGGCGTATCAGGCGATAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGcgcg                                                                                                              >  1:42839/1‑143 (MQ=255)

                             cGTATAAGGCGTATCAGGCGATAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGc                                                                                               <  2:642118/151‑1 (MQ=255)

                                         aTCAGGCGATAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGc                                                                                   >  1:582039/1‑151 (MQ=255)

                                                 aTAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGa                                                                           <  1:15729/151‑1 (MQ=255)

                                                 aTAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGa                                                                           <  2:52310/151‑1 (MQ=255)

                                                  tAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTg                                                                            >  1:634548/1‑149 (MQ=255)

                                                   aGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAgg                                                                         <  1:171289/151‑1 (MQ=255)

                                                       aTTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTc                                                                       >  1:462096/1‑149 (MQ=255)

                                                        ttttGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGt                                                                    >  1:80665/1‑151 (MQ=255)

                                                        ttttGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGt                                                                    >  1:109932/1‑151 (MQ=255)

                                                             cTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGTTTCAc                                                               >  1:238560/1‑151 (MQ=255)

                                                               aTCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGTTTCAcc                                                              <  2:78669/150‑1 (MQ=255)

                                                                   agaTAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGTTTCAccgcccg                                                         <  1:513132/151‑1 (MQ=255)

                                                                      tAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGTTTCAccgcccgc                                                        >  2:201246/1‑149 (MQ=255)

                                                                         aCGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGTTTCACCGCCCGCAGCAc                                                   >  2:150922/1‑151 (MQ=255)

                                                                            tCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGTTTCACCGCCCGCAGCAccgc                                                >  2:361612/1‑151 (MQ=255)

                                                                             ccTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGTTTCACCGCCCGCAGCAgcgcc                                               <  2:73278/151‑1 (MQ=255)

                                                                                  aCGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGTTTCACCGCCCGCAGCACCGCCGGAg                                           >  2:215824/1‑150 (MQ=255)

                                                                                  aCGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGTTTCACCGCCCGCAGCACCGCCGGAGt                                          >  1:565024/1‑151 (MQ=255)

                                                                                   cGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGTTTCACCGCCCGCAGCACCGCCGGAGtt                                         >  1:335589/1‑151 (MQ=255)

                                                                                      cGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTAt                                      <  2:179577/151‑1 (MQ=255)

                                                                                         tAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTATCa                                    <  2:326018/150‑1 (MQ=255)

                                                                                              aGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTATCAACCCAg                              <  2:246205/151‑1 (MQ=255)

                                                                                                     cGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTATCAACCCAGCCCGAcc                       <  1:312736/151‑1 (MQ=255)

                                                                                                             gCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTATCAACCCAGCCCGACCCATATAGc               >  1:626436/1‑151 (MQ=255)

                                                                                                                  cGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTATCAACCCAGCCCGACCCATATAGCAGtt           >  1:436111/1‑150 (MQ=255)

                                                                                                                  cGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTATCAACCCAGCCCGACCCATATAGCAGtt           >  2:631674/1‑150 (MQ=255)

                                                                                                                           gAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTATCAACCCAGCCCGACCCATATAGCAGTTAGCTAACTg  >  2:103628/1‑150 (MQ=255)

CCTTATCCGTCCTACGGGGTGGTGTTGTTCGTATAAGGCGTATCAGGCGATAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGTCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTATCAACCCAGCCCGACCCATATAGCAGTTAGCTAACTG  >  NC_000913/2177470‑2177742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A