Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F50 I2 R1	104	44.1	1376607	98.9%	1361464	150.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,307,916	C→T	D244N (GAT→AAT)	clsA ←	cardiolipin synthase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,307,916	0	C	T	100.0%	147.1 / NA	38	D244N (GAT→AAT)	clsA	cardiolipin synthase 1
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (18/20); total (18/20)

CGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAG  >  NC_000913/1307786‑1308063

cGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCgg                                                                                                                                 >  1:257068/1‑151 (MQ=255)

  ttttCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGgtg                                                                                                                               >  2:397607/1‑151 (MQ=255)

            tCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATg                                                                                                                      <  2:336557/150‑1 (MQ=255)

                   cccAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCGCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGAGATGTAATTAt                                                                                                               <  1:483001/150‑1 (MQ=255)

                    ccAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCg                                                                                                             <  1:515207/151‑1 (MQ=255)

                     cAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATc                                                                                                              >  1:323853/1‑149 (MQ=255)

                       gTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCgatc                                                                                                          <  1:571207/151‑1 (MQ=255)

                         cGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCgatca                                                                                                         <  2:218260/150‑1 (MQ=255)

                            aGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCgatcatgat                                                                                                     <  1:581737/151‑1 (MQ=255)

                                ataaataaTCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGatcatc                                                                                                 >  1:486478/1‑151 (MQ=255)

                                 taaataaTCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCt                                                                                                >  1:12399/1‑151 (MQ=255)

                                      aatCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCg                                                                                            <  2:488689/150‑1 (MQ=255)

                                           ccATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTg                                                                                      >  1:331894/1‑151 (MQ=255)

                                              tCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGgcg                                                                                   >  2:584988/1‑151 (MQ=255)

                                                         aTAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATAc                                                                        <  2:625150/151‑1 (MQ=255)

                                                            gggCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGc                                                                     <  1:512577/151‑1 (MQ=255)

                                                             ggCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCg                                                                    >  2:355201/1‑151 (MQ=255)

                                                                 ttCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTaa                                                                 >  1:224877/1‑150 (MQ=255)

                                                                  tCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTaaa                                                                >  2:99577/1‑150 (MQ=255)

                                                                        cgcgCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAacacac                                                         <  1:396274/151‑1 (MQ=255)

                                                                        cgcgCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAacacac                                                         <  1:342634/151‑1 (MQ=255)

                                                                          cgcCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGc                                                       >  1:116770/1‑151 (MQ=255)

                                                                              aTCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATc                                                    >  2:394005/1‑150 (MQ=255)

                                                                                 aGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATc                                                    >  2:235303/1‑147 (MQ=255)

                                                                                         ccATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCttt                                        <  2:686459/151‑1 (MQ=255)

                                                                                             tGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAg                                     <  1:417530/150‑1 (MQ=255)

                                                                                                  tACGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCg                               >  2:495106/1‑151 (MQ=255)

                                                                                                   aCGCCCGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTc                                <  2:409186/149‑1 (MQ=255)

                                                                                                      cccGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCa                           <  1:408895/151‑1 (MQ=255)

                                                                                                        cGCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACt                         >  1:346357/1‑151 (MQ=255)

                                                                                                         gCATCTTGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACtt                        >  2:551907/1‑151 (MQ=255)

                                                                                                              ttGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAAt                    >  2:643375/1‑150 (MQ=255)

                                                                                                              ttGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAAt                    >  1:524084/1‑150 (MQ=255)

                                                                                                               tGTTTGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAAt                    <  1:320171/149‑1 (MQ=255)

                                                                                                                   tGAAGTAGCGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCgg               <  1:577996/150‑1 (MQ=255)

                                                                                                                           cGAGGATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCa      <  1:655025/151‑1 (MQ=255)

                                                                                                                               gATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAg  <  2:626026/151‑1 (MQ=255)

                                                                                                                               gATTGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGAACATGATCAGCTTGCGATGTTGGCGTAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTCCAATACCGGCATTACGCATTa   <  1:143909/150‑1 (MQ=255)

CGTTTTCCGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAG  >  NC_000913/1307786‑1308063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A