Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	701,574	A→G	A7A (GCT→GCC)	nagC ←	N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	701,574	0	A	G	100.0%	120.5 / NA	34	A7A (GCT→GCC)	nagC	N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (11/23); total (11/23)

CGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGC  >  NC_000913/701434‑701721

cgcgTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGc                                                                                                                                           >  1:544151/1‑151 (MQ=255)

  cgTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCt                                                                                                                                         <  1:582485/151‑1 (MQ=255)

            gTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATAc                                                                                                                               <  1:365760/151‑1 (MQ=255)

             ttACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACt                                                                                                                              >  2:575000/1‑151 (MQ=255)

              tACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACt                                                                                                                              <  1:342626/150‑1 (MQ=255)

               aCGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACtt                                                                                                                             <  1:387032/150‑1 (MQ=255)

                      cGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTAt                                                                                                                     <  1:575015/151‑1 (MQ=255)

                        ggggCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTAtt                                                                                                                    <  2:180901/150‑1 (MQ=255)

                         gggCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGa                                                                                                                  <  2:580288/151‑1 (MQ=255)

                                  ggCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGAc                                                                                                          <  1:297640/150‑1 (MQ=255)

                                              cAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTtctc                                                                                                                         >  1:192967/1‑123 (MQ=255)

                                              cAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTtctc                                                                                                                         <  2:192962/123‑1 (MQ=255)

                                                    gAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACg                                                                                          <  2:112554/148‑1 (MQ=255)

                                                            gagaTTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGt                                                                               <  2:165988/151‑1 (MQ=255)

                                                                 tGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGAttt                                                                          >  1:181126/1‑151 (MQ=255)

                                                                    cccGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTaa                                                                       <  2:330118/151‑1 (MQ=255)

                                                                       gTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAAt                                                                    <  1:344371/151‑1 (MQ=255)

                                                                                       cGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGt                                                    <  1:258178/151‑1 (MQ=255)

                                                                                           aaaCCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTGCCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCa                                                  <  2:532304/149‑1 (MQ=255)

                                                                                              ccgccgCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTg                                             <  1:457821/151‑1 (MQ=255)

                                                                                                gccgcGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGc                                           <  2:486147/151‑1 (MQ=255)

                                                                                                 ccgcGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGATTGCAGTCAGGTTGGCt                                          <  2:279006/151‑1 (MQ=255)

                                                                                                 ccgcGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCt                                          <  2:328262/151‑1 (MQ=255)

                                                                                                    cgcTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACt                                       >  1:130418/1‑151 (MQ=255)

                                                                                                       tGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACttt                                     >  1:273763/1‑150 (MQ=255)

                                                                                                             gCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTgcg                              <  2:519050/151‑1 (MQ=255)

                                                                                                             gCTGTTTTACGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTgcg                              >  2:485714/1‑151 (MQ=255)

                                                                                                                     aCGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAgtgt                      >  2:412867/1‑151 (MQ=255)

                                                                                                                     aCGAGATCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAgtg                       <  1:260542/150‑1 (MQ=255)

                                                                                                                       gagaTCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGc                    >  1:155840/1‑151 (MQ=255)

                                                                                                                         gaTCAACATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGGGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGcc                   >  2:430371/1‑150 (MQ=255)

                                                                                                                             aaCATTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGAc              <  2:294609/151‑1 (MQ=255)

                                                                                                                                aTTACCTATCTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGtt           <  2:551219/151‑1 (MQ=255)

                                                                                                                                         cTGGGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGc  >  2:513625/1‑151 (MQ=255)

CGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTATTGAGTTACGACCTCGTTACCGTTAACGATGGTCTTGGTGATTTTAAAATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGC  >  NC_000913/701434‑701721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A