Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	741,429	A→G	S376S (TCT→TCC)	dtpD ←	dipeptide and tripeptide permease D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	741,429	0	A	G	100.0%	124.4 / NA	34	S376S (TCT→TCC)	dtpD	dipeptide and tripeptide permease D
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (13/21); total (13/21)

GATCGCGCCAGAAAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCC  >  NC_000913/741282‑741573

gATCGCGCCAGAAAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGggaa                                                                                                                                               <  2:23806/151‑1 (MQ=255)

        cAGAAAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGAcc                                                                                                                                       >  2:72489/1‑151 (MQ=255)

         aGAAAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGAcc                                                                                                                                       >  2:502226/1‑150 (MQ=255)

          gAAAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGAcc                                                                                                                                       >  2:492245/1‑149 (MQ=255)

               cagcaTGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACAtt                                                                                                                                <  1:380147/151‑1 (MQ=255)

                agcaTGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTg                                                                                                                               <  1:279491/151‑1 (MQ=255)

                agcaTGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTg                                                                                                                               >  2:574704/1‑151 (MQ=255)

                     gTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGa                                                                                                                           <  1:488494/150‑1 (MQ=255)

                      tAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGATGAGTGACCATACATTGCGGAcc                                                                                                                         >  2:82599/1‑151 (MQ=255)

                                 tAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCt                                                                                                               <  2:147850/150‑1 (MQ=255)

                                   aTACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCTGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCtt                                                                                                              >  1:14634/1‑149 (MQ=255)

                                     aCGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAgg                                                                                                          >  1:631327/1‑151 (MQ=255)

                                        ccGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTc                                                                                                        >  1:326865/1‑150 (MQ=255)

                                                       aTTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCgg                                                                                        <  2:111612/151‑1 (MQ=255)

                                                                   gTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCaggc                                                                            <  2:195916/151‑1 (MQ=255)

                                                                       tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCaggcca                                                                          >  1:41242/1‑149 (MQ=255)

                                                                             aCATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAggccaaggccaa                                                                   <  2:201287/150‑1 (MQ=255)

                                                                                            cGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCa                                                   <  1:621976/151‑1 (MQ=255)

                                                                                                 aaCAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATgc                                              <  2:135958/151‑1 (MQ=255)

                                                                                                  aCAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATgcg                                             <  1:319474/151‑1 (MQ=255)

                                                                                                   cAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATgcgc                                            >  2:576309/1‑151 (MQ=255)

                                                                                                     gTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCAc                                          <  2:311671/151‑1 (MQ=255)

                                                                                                        ccGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGt                                       >  1:130905/1‑151 (MQ=255)

                                                                                                          gCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTAc                                     <  1:621547/151‑1 (MQ=255)

                                                                                                                   aTCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTAccc                             >  1:122290/1‑150 (MQ=255)

                                                                                                                     cACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCcgcg                          <  2:88336/151‑1 (MQ=255)

                                                                                                                     cACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCcgc                           <  2:468095/150‑1 (MQ=255)

                                                                                                                            aGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACActct                   <  1:537016/151‑1 (MQ=255)

                                                                                                                             ggCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACActctc                  <  1:42211/151‑1 (MQ=255)

                                                                                                                               ccTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCtt                <  1:379201/151‑1 (MQ=255)

                                                                                                                                  aaTACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCtttt              <  1:482791/150‑1 (MQ=255)

                                                                                                                                      ccATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTacca          <  2:160561/150‑1 (MQ=255)

                                                                                                                                       cATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTaccacc        <  2:297846/151‑1 (MQ=255)

                                                                                                                                           aGTGGCAGGGAAGAGTGACCATACATTTCGGGCCAGCGGGCGCTTAAGGGCAGAATGCAGAATCCGGCGCTCATCAGGCCAAAGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGGTTACCCCCCACACTCTCTTTTAccacccac     >  2:284058/1‑150 (MQ=255)

                                                                                                                                              ggCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGcc  <  1:565796/150‑1 (MQ=255)

GATCGCGCCAGAAAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCTTTTACCACCCACGCC  >  NC_000913/741282‑741573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A