Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F50 I1 R1
|
170 |
39.5 |
1266514 |
99.2% |
1256381 |
150.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,321,083 |
G→A |
A101V (GCG→GTG) |
trpD ← |
fused glutamine amidotransferase (component II) of anthranilate synthase/anthranilate phosphoribosyl transferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,321,083 | 0 | G | A | 94.1%
| 122.5
/ ‑6.1
| 34 | A101V (GCG→GTG) | trpD | fused glutamine amidotransferase (component II) of anthranilate synthase/anthranilate phosphoribosyl transferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (12/20); minor base C (0/2); total (12/22) |
| Fisher's exact test for biased strand distribution p-value = 5.29e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TGGGCGTTGATGGTTAAACCGGCCGGAATGTTACTGCCAACCAGCGAGTGATAACGCGCCACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCGCCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAGGCAAATGCCAATAATGGGCAGCTTGCCACGCAAGCGGGTGAGGAGTTCCGGCATACAACCGGCTTCGCTCGGCACACCGGGGCCAGG > NC_000913/1320939‑1321219
|
tGGGCGTTGATGGTTAAACCGGCCGGAATGTTACTGCCAACCAGCGAGTGATAACGCGCCACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGAc > 2:253887/1‑151 (MQ=255)
gATGGTTAAACCGGCCGGAATGTTACTGCCAACCAGCGAGTGATAACGCGCCACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACAt > 2:597431/1‑149 (MQ=255)
tAAACCGGCCGGAATGTTACTGCCAACCAGCGAGTGATAACGCGCCACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGccccc < 1:420704/150‑1 (MQ=255)
aaaCCGGCCGGAATGTTACTGCCAACCAGCGAGTGATAACGCGCCACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGt < 1:330550/151‑1 (MQ=255)
cggccggAATGTTACTGCCAACCAGCGAGTGATAACGCGCCACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGc > 1:411858/1‑151 (MQ=255)
gccggAATGTTACTGCCAACCAGCGAGTGATAACGCGCCACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCtt < 2:233408/151‑1 (MQ=255)
ccggAATGTTACTGCCAACCAGCGAGTGATAACGCGCCACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTc < 1:297188/151‑1 (MQ=255)
ttACTGCCAACCAGCGAGTGATAACGCGCCACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCg > 1:582552/1‑151 (MQ=255)
gCCAACCAGCGAGTGATAACGCGCCACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTg < 2:137135/150‑1 (MQ=255)
gCCAACCAGCGAGTGATAACGCGCCACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTg > 2:130227/1‑150 (MQ=255)
aaCCAGCGAGTGATAACGCGCCACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCTGTAAGCTTCGACAATCGCCTGATGt > 1:516443/1‑151 (MQ=255)
aGCGAGTGATAACGCGCCACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGa < 1:251290/151‑1 (MQ=255)
gCGAGTGATAACGCGCCACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCg > 1:498820/1‑149 (MQ=255)
gATAACGCGCCACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAGGCaaa < 2:546861/150‑1 (MQ=255)
aaCGCGCCACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTGAGCTTCGACAATCGCCTGATGTCCGAGGCAAATGcc < 1:148039/151‑1 (MQ=255)
ccACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCTGTAAGCTTCGACAATCGCCTGATGTCCGAGGCAAATGCCaataat < 2:516432/151‑1 (MQ=255)
aCCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAGGCAAATGCCAATAATgg > 2:196408/1‑151 (MQ=255)
cGGCAGCGGGTTTGTTAATCCGGGAAAGATCGCGTGGCCGGCATGGTCAATGGTGGAGGCTTTAGCGTGGAGAATTTCGCCCCCCTGACCGGCAAAGCGTACGGAAGCGTAGTCAAGCGCCTGATGTCGGAGGCAAATGGCAATAATgg < 2:564569/149‑1 (MQ=255)
aGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAg > 2:95690/1‑128 (MQ=255)
aGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAg < 1:95690/128‑1 (MQ=255)
tGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAGGCAAATGCCAATAATGGGCAGCTTGCCACg < 2:411851/150‑1 (MQ=255)
tGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAGGCAAATGCCAATAATGGGCAGCTTGCCACg < 2:498810/150‑1 (MQ=255)
aaTCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAGGCAAATGCCAATAATGGGCAGCTTGCCACGCAAg < 2:625297/150‑1 (MQ=255)
aaTCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAGGCAAATGCCAATAATGGGCAGCTTGCCACGCAAGc > 1:28662/1‑151 (MQ=255)
ccGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAGGCAAATGCCAATAATGGGCAGCTTGCCACGCAAGCgg < 1:384945/150‑1 (MQ=255)
cGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAGGCAAATGCCAATAATGGGCAGCTTGCCACGCAAGCGGGt < 2:492966/151‑1 (MQ=255)
cAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCCCCTGACCGACATAGCCCCCGTAAGCTTCGCCAATCGCCTGATCTCCGAGGCAAATGCCAACCATGGGCAGCTTGCCACGCAAGCGGGTgag < 2:557669/151‑1 (MQ=255)
aTCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAGGCAAATGCCAATAATGGGCAGCTTGCCACGCAAGCGGGTGAGGAGtt < 2:110944/151‑1 (MQ=255)
cGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAGGCAAATGCCAATAATGGGCAGCTTGCCACGCAAGCGGGTGAGGAGTTcc < 2:229171/151‑1 (MQ=255)
gACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAGGCAAATGCCAATAATGGGCAGCTTGCCACGCAAGCGGGTGAGGAGTTCCGGCAt > 2:506631/1‑151 (MQ=255)
cATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAGGCAAATGCCAATAATGGGCAGCTTGCCACGCAAGCGGGTGAGGAGTTCCGGCATACAAcc < 2:90726/151‑1 (MQ=255)
gCTGGAGGCTTTACCGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAGGCAAATGCCAATAATGGGCAGCTTGCCACGCAAGCGGGTGAGGAGTTCCGGCATACAACCGGCTTCGCTc < 2:564780/151‑1 (MQ=255)
cGTGGAGAATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAGGCAAATGCCAATAATGGGCAGCTTGCTACGCAAGCGGGTGAGGAGTTCCGGCATACAACCGGCTTCGCTCGGCACACCGGGGcc < 2:253510/151‑1 (MQ=255)
gagaATTTCGCCCACCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAGGCAAATGCCAATAATGGGCAGCTTGCCACGCAAGCGGGTGAGGAGTTCCGGCATACAACCGGCTTCGCTCGGCACACCGGGGCCAgg > 1:93279/1‑150 (MQ=255)
|
TGGGCGTTGATGGTTAAACCGGCCGGAATGTTACTGCCAACCAGCGAGTGATAACGCGCCACCGGCAGCGGGTTTGTTAATCCGGCAAACATCGCCTGACCGTCATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCGCCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAGGCAAATGCCAATAATGGGCAGCTTGCCACGCAAGCGGGTGAGGAGTTCCGGCATACAACCGGCTTCGCTCGGCACACCGGGGCCAGG > NC_000913/1320939‑1321219
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
