Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,863,313	T→C	A181A (GCT→GCC)	gapA →	glyceraldehyde‑3‑phosphate dehydrogenase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,863,313	0	T	C	100.0%	128.4 / NA	34	A181A (GCT→GCC)	gapA	glyceraldehyde‑3‑phosphate dehydrogenase A
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (13/21); total (13/21)

CGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCTACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATG  >  NC_000913/1863178‑1863457

cGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGtt                                                                                                                                   >  1:512245/1‑151 (MQ=255)

    aaaTATGCTGGCCAGGACATCTTTTCCAACGCTTCCTGCCCCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATACCTCCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATg                                                                                                                               <  2:389046/151‑1 (MQ=255)

           cTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGtc                                                                                                                        >  2:562247/1‑151 (MQ=255)

                              cAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCgcggc                                                                                                     >  1:44953/1‑151 (MQ=255)

                                    ttCCTGCACCCCCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGCCCCCCGTTCACGCTCCTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGcc                                                                                                 <  1:314213/149‑1 (MQ=255)

                                       cTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCgcggc                                                                                            >  2:233690/1‑151 (MQ=255)

                                       cTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCgcggc                                                                                            >  2:114317/1‑151 (MQ=255)

                                               ccaACTGCCTGGCTCCGCTGGCTAATGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGAGGCCCCCCTTTCCCGCTACTTCCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCa                                                                                    <  1:423662/151‑1 (MQ=255)

                                                      ccTGGCTCCGCTGGCTAAATTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAAcat                                                                              <  2:44953/150‑1 (MQ=255)

                                                         ggCTCCGCGGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACatcatc                                                                          <  2:512234/151‑1 (MQ=255)

                                                             ccGCTGGCTAAAGTTATCAACGATAACTCCGGCATCATCGAAGGTCTGATGCCCCCCGTTCCCGCTCCTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATccc                                                                        <  1:502963/149‑1 (MQ=255)

                                                               gCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTcc                                                                    >  1:436049/1‑151 (MQ=255)

                                                                     tAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTAcc                                                              >  1:620158/1‑151 (MQ=255)

                                                                      aaaGTTATCAACGATAACTTCGGCATCATCGAAGGTCGGATGACCCCCGTTCCCGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTAcc                                                              <  2:34982/150‑1 (MQ=255)

                                                                       aaGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTCCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCgg                                                            <  2:139978/151‑1 (MQ=255)

                                                                         gTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGtg                                                          >  2:288685/1‑151 (MQ=255)

                                                                               aaCGATATCTGGGGCATCATCGAGGGTCTGATGACCACCTTTCACGCTCCTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTgctgct                                                     <  1:259233/150‑1 (MQ=255)

                                                                                     aaCTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTg                                              >  2:209829/1‑151 (MQ=255)

                                                                                        ttCGCCATCATCGAAGGTCTGATGCCCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGt                                             <  2:297791/149‑1 (MQ=255)

                                                                                          cGGCATCATCGAAGGTCTGATGCCCCCCTTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAg                                           <  2:262426/149‑1 (MQ=255)

                                                                                                catcGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGt                                    <  1:59429/150‑1 (MQ=255)

                                                                                                catcGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGt                                    >  2:21209/1‑150 (MQ=255)

                                                                                                   cGAAGGTCTGATGACCCCCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACt                                 <  1:312925/150‑1 (MQ=255)

                                                                                                                 ccaccGTTCCCGCTCCTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAAt                    <  1:143574/149‑1 (MQ=255)

                                                                                                                 ccaccGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATgg                  >  2:382818/1‑151 (MQ=255)

                                                                                                                      gTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAac             >  1:546515/1‑151 (MQ=255)

                                                                                                                       ttCCCGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAac             <  2:350325/150‑1 (MQ=255)

                                                                                                                         cACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAactg           <  2:346081/150‑1 (MQ=255)

                                                                                                                         cACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAac             <  2:244125/148‑1 (MQ=255)

                                                                                                                          aCGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAactgac         >  2:598785/1‑151 (MQ=255)

                                                                                                                           cGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAactgact        <  1:121534/151‑1 (MQ=255)

                                                                                                                             ctactaCCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAactgactg       <  1:583392/150‑1 (MQ=255)

                                                                                                                              tactacCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAactgactg       <  2:631793/149‑1 (MQ=255)

                                                                                                                                   ccGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATg  <  2:498097/149‑1 (MQ=255)

CGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCTACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATG  >  NC_000913/1863178‑1863457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A