Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,863,313	T→C	A181A (GCT→GCC)	gapA →	glyceraldehyde‑3‑phosphate dehydrogenase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,863,313	0	T	C	100.0%	125.1 / NA	34	A181A (GCT→GCC)	gapA	glyceraldehyde‑3‑phosphate dehydrogenase A
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/12); total (22/12)

AAGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCTACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGGCG  >  NC_000913/1863165‑1863460

aaGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCAc                                                                                                                                                   >  2:98742/1‑151 (MQ=255)

       tAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGaa                                                                                                                                             >  2:560677/1‑150 (MQ=255)

        aaCTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGaaa                                                                                                                                            >  1:548093/1‑150 (MQ=255)

           ttCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCg                                                                                                                                        >  1:319427/1‑151 (MQ=255)

                 aaaTATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGAt                                                                                                                                   <  2:212888/150‑1 (MQ=255)

                      tGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCg                                                                                                                             >  2:454639/1‑151 (MQ=255)

                                       tttCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGgcg                                                                                                            >  2:300803/1‑151 (MQ=255)

                                        ttCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGgcgc                                                                                                           >  2:99234/1‑151 (MQ=255)

                                        ttCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGgcgc                                                                                                           >  1:630988/1‑151 (MQ=255)

                                           cAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCgcggc                                                                                                        >  2:664816/1‑151 (MQ=255)

                                            aaCGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCgcggcg                                                                                                       >  2:28783/1‑151 (MQ=255)

                                                 ttCCTGCACCCCCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTGGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGcc                                                                                                    <  2:402351/149‑1 (MQ=255)

                                                        ccccccaACTGCCTGGCTCCGCGGGATAAAGTTATCAACTATAACTTGGGCATCATCTAGGTTCTGAGGCCCCCCGTTCCCGCAACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCtt                                                                                           <  2:578667/147‑1 (MQ=255)

                                                               aCTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGa                                                                                     >  1:14842/1‑150 (MQ=255)

                                                                          ccGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGt                                                                         >  2:392901/1‑151 (MQ=255)

                                                                            gCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTcc                                                                       >  2:64673/1‑151 (MQ=255)

                                                                               ggCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGCCCACCGTTCCCGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCctct                                                                    <  1:392897/151‑1 (MQ=255)

                                                                                     aGTTTTCAACGATAAATTCGGCATCATCGAAGGGCTGATGACCACCCTTCACGCTACTACCCCCACCCAGAAAAACGTTGATAGCCCCTCTCACAAAAACTGGCGCGGCGGCCGCCGCGCTTCCCAGAACATCATCCCGCCCTCTACCgg                                                               >  1:409456/1‑150 (MQ=255)

                                                                                            aaCGATAACTTCGGCATCATCGAAGGTCTGATGCCCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGtgctgc                                                         <  2:37928/149‑1 (MQ=255)

                                                                                              cGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTaa                                                      >  2:149930/1‑150 (MQ=255)

                                                                                                   aCTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTAACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGt                                                >  1:209119/1‑151 (MQ=255)

                                                                                                     ttCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGt                                                >  1:91007/1‑149 (MQ=255)

                                                                                                      tCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAgg                                             >  2:99185/1‑151 (MQ=255)

                                                                                                                  aaGGTTTGAGGACCCCCGTCCACGTTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACt                                    <  2:91008/148‑1 (MQ=255)

                                                                                                                  aaGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGcc                                 >  1:98036/1‑151 (MQ=255)

                                                                                                                        tggtccccccgtttCACGCTATTGCCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGTCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAg                               <  1:379163/135‑1 (MQ=255)

                                                                                                                         gatgcCCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAAc                            <  1:240654/149‑1 (MQ=255)

                                                                                                                                    ttaaccctactcCCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCaaa                 <  1:198606/143‑1 (MQ=255)

                                                                                                                                      cACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAactg              >  2:376911/1‑150 (MQ=255)

                                                                                                                                       aCGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAactgac            >  2:234220/1‑151 (MQ=255)

                                                                                                                                        cGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAactga             <  2:423367/149‑1 (MQ=255)

                                                                                                                                         gCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAactgactg          >  1:357244/1‑151 (MQ=255)

                                                                                                                                           tactacCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAactgactg          <  2:548098/149‑1 (MQ=255)

                                                                                                                                                 cGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGgcg  <  1:98740/151‑1 (MQ=255)

AAGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCTACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGGCG  >  NC_000913/1863165‑1863460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A