Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,923,990	A→G	V201A (GTA→GCA)	yebZ ←	inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,923,990	0	A	G	100.0%	114.5 / NA	31	V201A (GTA→GCA)	yebZ	inner membrane protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (19/12); total (19/12)

CATAACGATTAGCCAGCGCAATGACCACCATAATCATCACCAGGATGGCTTTGAGCAATAAGAGTTGGCCCCAGTAAGTCGTAAGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTACGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCACCGGCAGTAATCCCCCAAACCACGCCGCCGCACAAATCAGGTGA  >  NC_000913/1923853‑1924133

cATAACGATTAGCCAGCGCAATGACCACCATAATCATCACCAGGATGGCTTTGAGCAATAAGAGTTGGCCCCAGTAAGTCGTAAGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGc                                                                                                                                    <  1:430650/151‑1 (MQ=255)

  tAACGATTAGCCAGCGCAATGACCACCATAATCATCACCAGGATGGCTTTGAGCAATAAGAGTTGGCCCCAGTAAGTCGTAAGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCa                                                                                                                                   >  2:465052/1‑150 (MQ=255)

   aaCGATTAGCCAGCGCAATGACCACCATAATCATCACCAGGATGGCTTTGAGCAATAAGAGTTGGCCCCAGTAAGTCGTAAGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCaa                                                                                                                                  <  1:494946/150‑1 (MQ=255)

   aaCGATTAGCCAGCGCAATGACCACCATAATCATCACCAGGATGGCTTTGAGCAATAAGAGTTGGCCCCAGTAAGTCGTAAGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCaa                                                                                                                                  >  2:374784/1‑150 (MQ=255)

        ttAGCCAGCGCAATGACCACCATAATCATCACCAGGATGGCTTTGAGCAATAAGAGTTGGCCCCAGTAAGTCGTAAGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGcc                                                                                                                            >  1:496796/1‑151 (MQ=255)

          aGCCAGCGCAATGACCACCATAATCATCACCAGGATGGCTTTGAGCAATAAGAGTTGGCCCCAGTAAGTCGTAAGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCg                                                                                                                          <  2:310804/151‑1 (MQ=255)

                cgcAATGACCACCATAATCATCACCAGGATGGCTTTGAGCAATAAGAGTTGGCCCCAGTAAGTCGTAAGCGTGGGGGGAAGTCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAg                                                                                                                    <  2:566774/151‑1 (MQ=255)

                               aaTCATCACCAGGATGGCTTTGAGCAATAAGAGTTGGCCCCAGTAAGTCGTAAGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAgc                                                                                                     >  1:126332/1‑151 (MQ=255)

                                  catcaCCAGGATGGCTTTGAGCAATAAGAGTTGGCCCCAGTAAGTCGTAAGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGcc                                                                                                  <  1:230071/151‑1 (MQ=255)

                                         aGGATGGCTTTGAGCAATAAGAGTTGGCCCCAGTAAGTCGTAAGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAAt                                                                                             >  1:297096/1‑149 (MQ=255)

                                         aGGATGGCTTTGAGCAATAAGAGTTGGCCCCAGTAAGTCGTAAGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAg                                                                                           <  1:619347/151‑1 (MQ=255)

                                            aTGGCTTTGAGCAATAAGAGTTGGCCCCAGTAAGTCGTAAGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCt                                                                                        >  1:513744/1‑151 (MQ=255)

                                             tGGCTTTGAGCAATAAGAGTTGGCCCCAGTAAGTCGTAAGCGTGGGGGGAAATCCGGTAATCAACAACGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTg                                                                                       >  1:312426/1‑151 (MQ=255)

                                                   tGAGCAATAAGAGTTGGCCCCAGTAAGTCGTAAGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGgtg                                                                                    <  2:423844/148‑1 (MQ=255)

                                                   tGAGCAATAAGAGTTGGCCCCAGTAAGTCGTAAGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTc                                                                                  <  1:593804/150‑1 (MQ=255)

                                                   tGAGCAATAAGAGTTGGCCCCAGTAAGTCGTAAGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCg                                                                                 >  1:200155/1‑151 (MQ=255)

                                                     aGCAATAAGAGTTGGCCCCAGTAAGTCGTAAGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGcc                                                                               >  1:392722/1‑151 (MQ=255)

                                                        aaTAAGAGTTGGCCCCAGTAAGTCGTAAGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAAc                                                                            >  2:555344/1‑151 (MQ=255)

                                                                           aaGTCGTAAGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCAt                                                         >  2:335483/1‑151 (MQ=255)

                                                                               cGTAAGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATacac                                                     <  1:52759/151‑1 (MQ=255)

                                                                                  aaGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACAcc                                                    >  2:499742/1‑149 (MQ=255)

                                                                                   aGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCaga                                                 <  2:199438/151‑1 (MQ=255)

                                                                                           gggAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCGTACACCAGAGCACCGGc                                         <  1:549671/151‑1 (MQ=255)

                                                                                             gAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCACCGGc                                         >  2:396250/1‑149 (MQ=255)

                                                                                                aTCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCACCGGCAgca                                     >  1:476498/1‑148 (MQ=255)

                                                                                                           aacaaTGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCACCGGCAGTAATCCCCCAAAcc                         >  1:386766/1‑151 (MQ=255)

                                                                                                             caaTGCATTAAGCACGCCGCTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCACCGGCAGTAATCCCCCAAACCAc                       <  1:193871/151‑1 (MQ=255)

                                                                                                                           cgccgcTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCACCGGCAGTAATCCCCCAAACCACGCCGCCGCACaaa          >  1:620312/1‑150 (MQ=255)

                                                                                                                           cgccgcTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCACCGGCAGTAATCCCCCAAACCACGCCGCCGCACAAAt         >  1:43014/1‑151 (MQ=255)

                                                                                                                                cTTGCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCACCGGCAGTAATCCCCCAAACCACGCCGCCGCACAAATCAGGt    >  1:138587/1‑151 (MQ=255)

                                                                                                                                   gCCAGTGCGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCACCGGCAGTAATCCCCCAAACCACGCCGCCGCACAAATCAGGTGa  >  1:79199/1‑150 (MQ=255)

CATAACGATTAGCCAGCGCAATGACCACCATAATCATCACCAGGATGGCTTTGAGCAATAAGAGTTGGCCCCAGTAAGTCGTAAGCGTGGGGGGAAATCCGGTAATCAACAATGCATTAAGCACGCCGCTTGCCAGTACGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCACCGGCAGTAATCCCCCAAACCACGCCGCCGCACAAATCAGGTGA  >  NC_000913/1923853‑1924133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A