Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,117,002	T→C	T42T (ACA→ACG)	wcaL ←	putative glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,117,002	0	T	C	100.0%	108.9 / NA	31	T42T (ACA→ACG)	wcaL	putative glycosyl transferase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (7/24); total (7/24)

GCGCCTGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGTGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATG  >  NC_000913/2116854‑2117142

gcgcCTGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTtgcgt                                                                                                                                            <  2:504132/151‑1 (MQ=255)

 cgcCTGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTtgcgtg                                                                                                                                           >  2:83466/1‑151 (MQ=255)

                   aCGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTtgcgt                                                                                                                                            >  2:335546/1‑132 (MQ=255)

                   aCGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTtgcgt                                                                                                                                            <  1:335553/132‑1 (MQ=255)

                        gAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGaa                                                                                                                     <  1:146106/150‑1 (MQ=255)

                         aaTGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAAtc                                                                                                                   >  2:334608/1‑151 (MQ=255)

                              cgcgCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTAc                                                                                                              <  2:523539/151‑1 (MQ=255)

                                cgcAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCt                                                                                                            <  2:584172/151‑1 (MQ=255)

                                 gcAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTc                                                                                                           <  2:213722/151‑1 (MQ=255)

                                      gTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATc                                                                                                      <  1:457447/151‑1 (MQ=255)

                                         tggctggcTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATccc                                                                                                    >  1:232315/1‑150 (MQ=255)

                                              ggctCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCatat                                                                                                <  1:613423/149‑1 (MQ=255)

                                              ggctCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCatat                                                                                                <  1:83105/149‑1 (MQ=255)

                                                  cGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATa                                                                                           <  1:604857/150‑1 (MQ=255)

                                                      ggCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAAcg                                                                                       <  1:227517/150‑1 (MQ=255)

                                                               tCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTg                                                                             >  2:424288/1‑151 (MQ=255)

                                                                cGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTgg                                                                            >  1:361427/1‑151 (MQ=255)

                                                                cGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTg                                                                             <  2:581443/150‑1 (MQ=255)

                                                                       ttGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAg                                                                       <  2:232310/149‑1 (MQ=255)

                                                                                   tCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTGTTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCt                                                          <  2:435266/150‑1 (MQ=255)

                                                                                   tCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCt                                                          <  2:71912/150‑1 (MQ=255)

                                                                                   tCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTg                                                         >  1:444213/1‑151 (MQ=255)

                                                                                    cGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCt                                                          <  2:239116/149‑1 (MQ=255)

                                                                                            aaCCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGc                                                 <  2:161045/150‑1 (MQ=255)

                                                                                               cAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGa                                             <  2:462577/151‑1 (MQ=255)

                                                                                                 gCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGaa                                            <  2:356296/150‑1 (MQ=255)

                                                                                                 gCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAAt                                           <  1:120552/151‑1 (MQ=255)

                                                                                                     gTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCa                                       <  1:28264/151‑1 (MQ=255)

                                                                                                       tCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAg                                      <  2:274088/150‑1 (MQ=255)

                                                                                                          ggCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGt                                     <  1:154009/148‑1 (MQ=255)

                                                                                                                        aTTTCGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCt                     <  2:338079/150‑1 (MQ=255)

                                                                                                                            cGTCCATGCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCAt                 <  1:537876/150‑1 (MQ=255)

                                                                                                                                   gCCGCGTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCt          <  2:211941/150‑1 (MQ=255)

                                                                                                                                     cgcgTGGGTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTccc       >  1:81956/1‑151 (MQ=255)

                                                                                                                                          gggTGTTTTGCGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATg  >  1:433536/1‑151 (MQ=255)

GCGCCTGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGTGTGTCGCCTTTTTGCAGCGCGAGAATCTCTACCTCAAATCCCATATCAATAAACGCGGTAATTTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATG  >  NC_000913/2116854‑2117142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A