Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F111 I1 R1 16 82.1 2903858 91.9% 2668645 142.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,196,305 C→T R395C (CGT→TGT)  icd → isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,196,3050CT97.9% 270.0 / NA 94R395C (CGT→TGT) icdisocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base T (57/34);  total (60/34)
Fisher's exact test for biased strand distribution p-value = 5.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTT  >  NC_000913/1196167‑1196444
                                                                                                                                          |                                                                                                                                           
gACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCt                                                                                                                                         >  1:161233/1‑143 (MQ=255)
gACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCt                                                                                                                                         >  1:701436/1‑143 (MQ=255)
  cAAAGGAAGTCCGGGCTCTATTAGTCGCGTCGCTGGGAGGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTga                                                                                                                                       <  2:896095/143‑1 (MQ=21)
   aaaGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTgat                                                                                                                                      >  1:413531/1‑143 (MQ=255)
   aaaGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCGGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTgat                                                                                                                                      <  2:572133/143‑1 (MQ=255)
   aaaGCACATCCTGGCTATATTATTCCCGCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTgat                                                                                                                                      <  1:214282/143‑1 (MQ=25)
     aGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAGGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTgatgg                                                                                                                                    >  1:380659/1‑143 (MQ=255)
      gTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCGCATGGGTTGGACCCAAGCGGCTTATTCCAGTGTTAGAGGTATGGAAGGGGCAATCAACGCGAAAACCGTAACCTATGACTTCGCGCGCCTcatgga                                                                                                                                   >  2:1028923/1‑143 (MQ=255)
      gTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTGAAGGTATGGAGGGCGCAATCAACGCGAAAACCGTAACCCACGACTTCGAGTGTATgatgga                                                                                                                                   >  2:1133821/1‑143 (MQ=255)
      gTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTCTGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTAGAGAGTCTgatgga                                                                                                                                   >  1:1080014/1‑143 (MQ=255)
         aaTCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGatggatgg                                                                                                                                <  1:900636/143‑1 (MQ=255)
           gccTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGgcg                                                                                                                              <  1:867718/142‑1 (MQ=255)
             cTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGAATTCGAGTGTCGGGTGGAGGGCGCt                                                                                                                            >  1:1201607/1‑143 (MQ=25)
               ggCTCTTTTGTGCTGTCCGGTGAGATGATGCTGCGCCACATGGTTTGGACCGAGGCGGCTGACTTAATTGTTAAGGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTaa                                                                                                                          <  2:411431/143‑1 (MQ=18)
                 ctctATTGTTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAAc                                                                                                                        <  1:1248560/143‑1 (MQ=255)
                 ctctATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGACGCGGCTGACTTCATTGTTCAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGCTGGAAGGCGCTAAAc                                                                                                                        >  2:1223593/1‑143 (MQ=21)
                     attattCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGGTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTACGAGTGTCTGATGGATGGCGCTACActgca                                                                                                                    >  2:496964/1‑142 (MQ=255)
                      ttattCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAActgctg                                                                                                                   >  1:539851/1‑143 (MQ=255)
                        attCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGaa                                                                                                                 <  2:1249874/143‑1 (MQ=255)
                         ttCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTACTTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGaaa                                                                                                                >  2:552573/1‑143 (MQ=255)
                         ttCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGaaa                                                                                                                >  1:495560/1‑143 (MQ=255)
                            tctcCGCTGAGATGATGCTGCGCCACATGGAACGGCCCGGAGCGGCGGGCGGAGTTGGTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGACATGt                                                                                                             >  1:615102/1‑143 (MQ=21)
                                   tGAGATGATGCTGCGCCACATGGGTTGGACCGAAACGGCTGACACTAATGCTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGt                                                                                                      >  2:1415542/1‑143 (MQ=18)
                                     agaTGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGttt                                                                                                    <  1:952740/143‑1 (MQ=255)
                                      gatgatGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTg                                                                                                   <  1:64987/143‑1 (MQ=255)
                                       atgatgCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTgg                                                                                                  <  1:1141351/143‑1 (MQ=255)
                                           tgctgcGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATCGATGGCGCTAAACTGCGGAAATGTTCACAGCTTGGTGAc                                                                                              >  2:1218114/1‑143 (MQ=18)
                                            gctgcGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGCAATGTTCAGAGTTTGGTGAcg                                                                                             >  2:202718/1‑143 (MQ=255)
                                            gctgcGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGACATGTTCAGAGTTTGGTGagg                                                                                             >  2:416577/1‑141 (MQ=255)
                                             ctgcGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGAcgc                                                                                            <  1:1035080/143‑1 (MQ=255)
                                             ctgcGCCACATGGGTCGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGAcgc                                                                                            <  1:861740/143‑1 (MQ=255)
                                              tgcGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGTGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGAcgcg                                                                                           >  1:823432/1‑143 (MQ=255)
                                              tgcGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGAcgcg                                                                                           >  2:1430336/1‑143 (MQ=255)
                                                  ccACATGGGTTGGACCGAAGCGGCTGACTTAAGTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGatca                                                                                       <  1:960543/143‑1 (MQ=255)
                                                  ccACACGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGatca                                                                                       <  1:1145195/143‑1 (MQ=255)
                                                   cacaTGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGatcat                                                                                      <  2:836671/143‑1 (MQ=255)
                                                   cacaTGGGTTGGACCGAAGCGCCTGACTTAATTGTTCAAGATATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACCGCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGCTAGGTGACGCCatcct                                                                                      >  2:711364/1‑141 (MQ=255)
                                                    acaTGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGGGTTTGGTGACGCGatcagc                                                                                     >  1:1158028/1‑141 (MQ=255)
                                                        ggggTGGAGCGGAGCGGCTGGCTTAATGGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaaa                                                                                 <  2:1083693/143‑1 (MQ=18)
                                                           ttGGACCGAAGCGGCTGACTTACTCGATAAAGGTATGGAAGGCGCAATCAACGCGCGAACCGTAACCCATGACGTCGAGTGTCTGCTGGATGGCGCTAAACTGCTGAGATGTGCAGAGGTTGGTGACGCCGTCACCGagacca                                                                              >  2:1035467/1‑138 (MQ=18)
                                                           ttGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACa                                                                              >  1:188018/1‑143 (MQ=255)
                                                           ttGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGGTTTGTGACGCGATCATCGAAGACa                                                                              >  2:795334/1‑143 (MQ=255)
                                                            tgcgccGAGGCGGCTGACTTAATTGTTGAAGCTATGGAAGGCGCAATCAACCCGAAAACCGCAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAACCAt                                                                             <  1:403094/139‑1 (MQ=17)
                                                            tGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGACGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGCGGGCGCTAAACTGCTGACATGCTCAGCGTCTGGGGACGCGGTCATCGCAAAGAt                                                                             >  2:438284/1‑143 (MQ=255)
                                                              gagcGAAGCGGCTGACTTAATTGTTAGAGGTATGGAAGGCGCAATGAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGt                                                                           <  2:981641/140‑1 (MQ=255)
                                                               aCCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGACAACATGTa                                                                          >  1:177445/1‑143 (MQ=255)
                                                               aCCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGGCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGtc                                                                          >  2:1173522/1‑142 (MQ=255)
                                                                ccGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTaa                                                                         >  2:66470/1‑143 (MQ=255)
                                                                  gaggCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATg                                                                       <  2:380659/140‑1 (MQ=255)
                                                                  caagtgtcTGACTTAATTGTTAAAGGTATGTAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGACTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATg                                                                       <  2:531488/136‑1 (MQ=17)
                                                                        gCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACAGGTAATGCCGTAg                                                                 >  2:35981/1‑143 (MQ=255)
                                                                        gCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTACGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAg                                                                 >  2:911067/1‑143 (MQ=255)
                                                                         cTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAATCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGt                                                                >  2:758699/1‑143 (MQ=255)
                                                                          tGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGtt                                                               >  1:105695/1‑143 (MQ=255)
                                                                          tGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACCTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCAATCATCGAAAACATGTAATTCCGTAGtt                                                               >  2:719527/1‑143 (MQ=255)
                                                                           gACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGttt                                                              >  1:777647/1‑143 (MQ=255)
                                                                           gACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGttt                                                              <  1:552573/143‑1 (MQ=255)
                                                                            aCTTAATTGTTAAAGGCATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTg                                                             <  1:900505/143‑1 (MQ=255)
                                                                              ttAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGtt                                                           >  2:467774/1‑143 (MQ=255)
                                                                              ttAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGGTGGtt                                                           >  1:1078803/1‑143 (MQ=255)
                                                                                  ttGTTAAAGGTATGTAAGGCGCAATCCACGCGAAGACCGTAACCTATGACTTCGTGTGTCTGATGGAGGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAAt                                                       <  2:1302367/143‑1 (MQ=17)
                                                                                  ttGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAGCCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAAt                                                       <  2:126880/143‑1 (MQ=255)
                                                                                  ttGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGGAAACACGTAATGCCGTAGTTTGTTACAt                                                       >  2:379615/1‑143 (MQ=255)
                                                                                   tGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAAtt                                                      >  2:174233/1‑143 (MQ=255)
                                                                                     ttAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTTGTTTGTTCAAtttt                                                    >  1:1381431/1‑142 (MQ=255)
                                                                                     ttAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATtta                                                    >  1:427179/1‑143 (MQ=255)
                                                                                     ttAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATtta                                                    >  1:924416/1‑143 (MQ=255)
                                                                                      tAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATttat                                                   <  1:1415542/143‑1 (MQ=255)
                                                                                       aaaGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCGGTAGGTTGTTCATTatatt                                                  >  2:468122/1‑143 (MQ=18)
                                                                                            tATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCGAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCACCGCAAACATGTAATGCCGTAGTTTGTTAAATTTATTAAAgg                                             >  2:704644/1‑143 (MQ=21)
                                                                                              tGGAAGGCGCAATCAACGCGAAAACCGTAACCTACGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGa                                           <  1:911067/143‑1 (MQ=255)
                                                                                              cggAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGa                                           <  2:613625/142‑1 (MQ=255)
                                                                                                gAAGGCGCAATCAACGCGAAAACCGTAACCTATTACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGACAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGc                                         >  2:1267309/1‑143 (MQ=255)
                                                                                                 aaGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTAATGCATTCGTCAGACAATCAGAAGGCGGTCTTCGAAATCATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCg                                        <  1:672687/143‑1 (MQ=255)
                                                                                                   ggagcAATCAACGCGATGCCCGTAACCGATGCCTTCGAGTGTCTGATGGATGGCGCTAAGCTGCTGAAATGTTCAGTGGTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTa                                      <  2:691900/140‑1 (MQ=21)
                                                                                                     cgcAATCAACGCGAAAACCGTAACCCATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAAc                                    <  1:1239092/143‑1 (MQ=255)
                                                                                                      gcAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACg                                   >  1:1274825/1‑143 (MQ=255)
                                                                                                      gcAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTAATGGATGGCGCTAAACCGCTGAAATGTTCAGAGTTTGGTGACGCGATAAACAAAAACATGGAATGACGACCTTTTTGAAATTTATTAAGGGGACCCTAACg                                   >  2:1085513/1‑143 (MQ=18)
                                                                                                          tCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCTCAGTTTGGTGACGCGCTCATCGAAAACATGTAATGCCGTAGTTCGTTCAGTTTATTAACGGGGGCGTAACGCTcc                               >  1:1091733/1‑143 (MQ=255)
                                                                                                           cAACGCGACAGCCGTAACCTATGATTTCGGGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTCTGGTGACGCTATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTccc                              <  2:579541/143‑1 (MQ=25)
                                                                                                            aaCGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTTACGCTCCCg                             <  1:851413/143‑1 (MQ=255)
                                                                                                             aCGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTGCAGAGGTGGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTGTGTTAAATGTATTAACGGGAGGGTAACGCTCCCgt                            >  1:102239/1‑143 (MQ=25)
                                                                                                              cgcgAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCAGCGAAAACATGTAATGCCGTCGTTTGTTAAATTTATTAATGGGAGCGTAACGCTCCCgct                           >  2:124892/1‑141 (MQ=25)
                                                                                                               gcgAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGAACATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCgttg                          >  2:989711/1‑143 (MQ=255)
                                                                                                                 gAACACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCgttgtt                        <  2:449957/143‑1 (MQ=255)
                                                                                                                    aaCCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGttttt                     >  1:288648/1‑143 (MQ=255)
                                                                                                                              tATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGgctg           >  1:861204/1‑143 (MQ=255)
                                                                                                                               aTGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGgctgc          >  1:1355434/1‑143 (MQ=255)
                                                                                                                                tGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGgctgct         >  1:557327/1‑143 (MQ=255)
                                                                                                                                tGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGgctgct         >  1:1267482/1‑143 (MQ=255)
                                                                                                                                    ttCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTACCGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACg     >  1:49087/1‑143 (MQ=255)
                                                                                                                                    ttCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGAAATGCCGTAGTTTGTTAAATCTATTAACGGGACCGTAACGCTCACGTTGTTTTCTGTTCGgctgcgcagg     >  2:706483/1‑138 (MQ=25)
                                                                                                                                    ttCGAGTGTCTGATGGATGGCGCTAAACTGCAGAAAGGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACg     >  2:880872/1‑143 (MQ=255)
                                                                                                                                       gAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTGTTTTGTTAGGCTGCTAACTGtt  >  2:629738/1‑143 (MQ=255)
                                                                                                                                          |                                                                                                                                           
GACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTT  >  NC_000913/1196167‑1196444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A