Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F17 I0 R1 40 55.2 1410130 81.3% 1146435 215.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,196,305 C→T 100% R395C (CGT→TGT)  icd → isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,196,3050CT97.6% 130.3 / ‑6.0 49R395C (CGT→TGT) icdisocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (26/18);  minor base G (3/1);  total (30/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAG‑CGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTA‑GGCTGCTAACGGTTATCAAAATTTTATCAAAAAAAGTTATCAAAACCCCTCGGTAGTTTTGGGGTAGGCTGGCCGGTCAGGTGGTAGTTCTACTACTA  >  NC_000913/1196089‑1196528
                                                                                                                                                                                                                         |                                                                                                                                                                                                                                
gCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAG‑TGTCTgatgga                                                                                                                                                                                                                        <  1:383775/227‑1 (MQ=255)
      ggTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGCAAACCGTAACCTATGACTTCGAG‑GGTCTGATGGATAGCGCt                                                                                                                                                                                                                 >  1:215255/1‑228 (MQ=255)
      ggTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAG‑TGTCTGATGGATGGCGCt                                                                                                                                                                                                                 >  1:28285/1‑228 (MQ=255)
      ggTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAAATGTTAAAGGAATGGAAGGCGCAATCAACGCGAAAACCGTAACCTTTGACTTCGAG‑GGTCTGATGGATGgcgtc                                                                                                                                                                                                                 >  1:69546/1‑226 (MQ=255)
                  ggTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTAGGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAG‑TGTCTGATGGATGGCGCt                                                                                                                                                                                                                 >  2:491275/1‑216 (MQ=255)
                   gTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAG‑TGTCTGATGGATGGCGCTa                                                                                                                                                                                                                <  1:491275/216‑1 (MQ=255)
                        gAAGGCGCCCTTTTTGAAGCCACCCACGGTCCTGCGCCGAAATAGGTCGGTCAGGACAAAGTAAATCCGGGCTCTATTATTCGCTCCGCTGAGATGATGCTGCGACACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAG‑TGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCa                                                                                                                                                                                               <  2:69546/228‑1 (MQ=37)
                                   ttttGAAGCCCCCCCCGGTACTGCTCCGAAATAGGCCGGTAAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAG‑TGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGa                                                                                                                                                                                    <  2:215255/227‑1 (MQ=255)
                                     ttcgagtcacccccGGGTCTGCGCCTAAATCGGCCGGGCAGGCCAAAGGAAATACGTGCTCTATTTGTCGCGCGGCTGGGATGATGCTGAGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAT‑TGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGAcg                                                                                                                                                                                  <  2:201709/221‑1 (MQ=18)
                                              cccacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAG‑TGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGGTCATCg                                                                                                                                                                         >  1:456913/1‑228 (MQ=255)
                                                cccGGTTCTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCGCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAG‑TGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGa                                                                                                                                                                        <  1:7438/225‑1 (MQ=255)
                                                               aaaTATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGGAACCTATGACTTCGAG‑TGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGCGTTTGGTGACGCGATCATCGAAAAAATGAAATAccta                                                                                                                                                        >  1:209881/1‑226 (MQ=255)
                                                                      ccGGTTAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAG‑TGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTg                                                                                                                                                  <  1:642680/227‑1 (MQ=255)
                                                                                   ggTAAATCCTGGCTCTATTAATCTCTCCGAGTAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAATCCGTAACCTATGACTTCGAG‑TGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAAc                                                                                                                                    <  1:702840/227‑1 (MQ=255)
                                                                                   aGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCAACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGAATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAG‑TGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGCATTTGGTGACGCGATTATCGAAAACATGTAATGCCGTCGTTTTGTAACGTTATTaa                                                                                                                                     >  1:288271/1‑227 (MQ=255)
                                                                                             ggCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAG‑TGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGTTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGATCGTaa                                                                                                                          >  1:639204/1‑228 (MQ=255)
                                                                                              gCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTGAATTTGTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTTACCTATGACTTCGAG‑TGTCTGATGGAGGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATg                                                                                                                                                                 <  1:33921/188‑1 (MQ=255)
                                                                                               ctctATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAG‑TGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTACATTTATTAACGGGAGCGTAACg                                                                                                                        >  1:34564/1‑228 (MQ=255)
                                                                                                        tctctcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAG‑TGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGGGTAACGCTCCCgttg                                                                                                               >  1:511078/1‑228 (MQ=255)
                                                                                                            tcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAG‑TGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAACACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGttt                                                                                                            >  1:332588/1‑227 (MQ=255)
                                                                                                            tcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAG‑TGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGACGTAGTTTGTTAAATTTATTAAAGGGGGCGAAACGCTCCCGTTGttt                                                                                                            >  2:406798/1‑227 (MQ=255)
                                                                                                              cGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAG‑TGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGGTGtttttt                                                                                                         >  1:515906/1‑228 (MQ=255)
                                                                                                                   agagtatgCGGGGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAG‑TGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTA‑g                                                                                                   <  1:617410/223‑1 (MQ=255)
                                                                                                                        atgCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAG‑CGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGAAACGACCCCGTTGTTTTTTGTTA‑Ggctgc                                                                                              >  1:551474/1‑228 (MQ=255)
                                                                                                                          gctgcGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGCCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAG‑TGTCTGATGGATGGCGCTAAACTGATGAAATGTTCAGAGTTTGGTGCCGCGATCATCGAAACCTTGTAATGCCGAAGTTTGTTAATTTTATTAACGGGGACGACACGCTCCCGCTGTTTTTGTTTA‑GGTTGCTa                                                                                            >  2:443784/1‑228 (MQ=255)
                                                                                                                                                 aaGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCCATCAACGCGAAAACCGTAACCTATGACTTCGAG‑TGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTa                                                                                                                                               <  1:240276/155‑1 (MQ=255)
                                                                                                                                                 aaGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAG‑TGTCTGATGGATAGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTa                                                                                                                                               >  2:240276/1‑155 (MQ=255)
                                                                                                                                                  aGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAG‑TGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGGAATGCCGTAGTTTGTTAAATTTATTAACGGAAGCGTAACGCACCCGTTGTTTTTTGTTA‑GTCTGCTAAGTGTTATCAAAATTTTATCaaaa                                                                    >  2:504453/1‑228 (MQ=255)
                                                                                                                                                                    tAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAG‑TGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTA‑GGCTGCTAACGGTTATCAAAATTTTATCAACAAAAGTTATCAAAACCCCt                                                  >  1:170884/1‑228 (MQ=255)
                                                                                                                                                                    tAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAG‑TGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTA‑GGCTGCTAACGGTTATCAAAATTTTATCAAAAAAAGTTATCAAAACCCCt                                                  <  1:39226/228‑1 (MQ=255)
                                                                                                                                                                    tAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAG‑TGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTA‑GGCTGCTAACGGGTATCAAAATTTTATCAAAAAATATTTTCAAAAccccc                                                  >  2:416451/1‑227 (MQ=255)
                                                                                                                                                                    tAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAG‑TGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTA‑GACTGCTAACGGTGATCAAAACTTTATCAATAAAAGTTATCAAAAACCCt                                                  >  1:320218/1‑228 (MQ=255)
                                                                                                                                                                      aaGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAG‑TGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTA‑GGCTGCTAACGGTTATCAAAATTTTATCAAAAAAAGTTATCAAAACCCCTCg                                                >  1:424841/1‑228 (MQ=255)
                                                                                                                                                                       tggTTTGGGAGGCGGAAGTCACGCTAAAACCGTAACCTAGGGCTTCGAG‑TGTCTGGTGGGTGGCGCTAACCTGCAGAAATGTTCAGAGTTTGGTGACGCCATCAGCGAAAACATGTAATGTCTTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTA‑GGCTGCTAACGGTTATCAAAATTTTATCAAAAAAAGTTATCAAAACCCCTCgg                                               <  2:288271/227‑1 (MQ=255)
                                                                                                                                                                            tGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAG‑TGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAACCATGTAATGCCGTAGTTTGTTAAATTTATTAACGGTAGCGTACCGCTCCCGTTGTTTTTTGTTA‑GGATGCTTACGGTTATCAGAATTTTTTCAAAAAAATTTATCAAAAACCCTCgggtgtt                                          >  2:343871/1‑223 (MQ=255)
                                                                                                                                                                            tGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAG‑TGTCTGATGGATGGCGCTAAACTGCTGAAATGATCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCG‑TGTTTTTTGGTAGGGCTGCTAACGGTGATCAAAATTTTATCTAAAAAAGTTATCAAACACACTCGTTAg                                            >  1:306395/1‑226 (MQ=255)
                                                                                                                                                                            tGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAG‑GGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGATTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTccag                                                                                                                  >  1:305950/1‑155 (MQ=255)
                                                                                                                                                                             ggAACGCGCAGTCAACGCGAAAACCGTAACCTATGACTTCGAG‑TGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCgt                                                                                                                 <  2:305950/157‑1 (MQ=255)
                                                                                                                                                                                   cgcAATCAACGCGAAAACCGTAACCTATGACTTCGGG‑TGTCTGATGGATGACGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACCCGATCATCGAAAACATGTAATGACGTAGTTTGTTAAATTTGTTAACGAGAGCGTAACGCTCCCGTTGTTTTTTGTTA‑GGCTGCTAACTGTTATCAAAATTTTTTATAAAAAATTTGTAAAAACCCCTCTGGAGTTTGTGGGt                                   >  2:697991/1‑228 (MQ=255)
                                                                                                                                                                                   cgcAATCAACGCGAAAACCGTAACCTATGACTTCGAG‑TGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTA‑GGCTGCTAACGGTTATCAAAATTTTTTCCAAAAAAGTTATCa                                                          >  2:5545/1‑205 (MQ=255)
                                                                                                                                                                                   cgcAATCAACGCGAAAACCGTAACCTATGACTTCGAG‑TGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTA‑GGCTGCTAACGGTTATCAAAATTTTATCAAAAAAAGTTATCAAAACCCCTCGGTGGTTTTGGGGt                                   >  1:232968/1‑228 (MQ=255)
                                                                                                                                                                                   cgcAATCAACGCGAAAACCGTAACCTATGACTTCGAG‑TGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTA‑GGCTGCTAACGGTTATCAAAATTTTATCAAAAAAAGTTATCAAAACCCCTCGGTAGTTTTGGGGt                                   >  1:69216/1‑228 (MQ=255)
                                                                                                                                                                                    gcAATCAACGCGAAAACCGTAACCTATGACTTCGAG‑TGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTA‑GGCTGCTAACGGTTATCAAAATTTTATCAAAAAAAGTTATCaa                                                         <  1:5545/205‑1 (MQ=255)
                                                                                                                                                                                               aaaaaCCGTAACCTTTTGCTTCGGGTTGTCT‑ATGGATGGCGCTAAACTGCGTAAATGTTCAGAGTTTGGTGTCGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTA‑GGCTGCTAACGGTTATCAAAATTTTATCAAAAAAAGTTATCAAAACCCCTCGGTAGTTTTGGGGTAGGCTGGCCGGt                       <  2:28427/227‑1 (MQ=255)
                                                                                                                                                                                                    ccGTAACCTATGACTTCGAG‑TGTCTGATGGATGGCGCTAAACTGCTGAAATGGTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTA‑GGCTGCTAACGGTTATCAAAATTTTATCAAAAAAAGTTATCAAAACCCCTCGGTAGTTTTGGGGTAGGCTGGCCGGTCAggt                  <  1:182604/228‑1 (MQ=255)
                                                                                                                                                                                                      gTAACCTATGAATTCGAG‑GGTCTGATGGTTGGCGCAAAACTTCTTAAATGTTCAGAGTTTGGTTACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTA‑GGCTGCTAACGGTTATCAAAATTTTATCAAAAAAAGTTATCAAAACCCCTCGGTAGTTTTGGGGTAGGCTGGCCGGTCAggtgg                <  2:639204/228‑1 (MQ=255)
                                                                                                                                                                                                                    cGAG‑TGTCTTAGGGAAGGCGGTAAACTGCTGAAATGTTCAGAGTATGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTA‑GGCTGCTAACGGTTATCAAAATTTTATCAAAAAAAGTTATCAAAACCCCTCGGTAGTTTTGGGGTAGGCTGGCCGGTCAGGTGGTAGTTctactacta  <  1:443784/228‑1 (MQ=255)
                                                                                                                                                                                                                    cGAG‑TGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGACAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTA‑GGTTGCTAACGGTTATCAAAATTTTATCCACAAAAGTTTTTAAAACCCCCCGGAATTTTTGGGGTAGGCTGGCAGGGCGAGTGGTGGTTcatctaata  >  2:490094/1‑220 (MQ=255)
                                                                                                                                                                                                                    cGAG‑TGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTA‑GGCTGCTAACGGTTATCAAAATTTTATCAAAAAAAGTTATCAAAACCCCTCGGTAGTTTTGGGGTAGGCTGGCCGGTCAGGTGGTAGTTctactacta  >  1:100362/1‑228 (MQ=255)
                                                                                                                                                                                                                         |                                                                                                                                                                                                                                
GCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAG‑CGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTA‑GGCTGCTAACGGTTATCAAAATTTTATCAAAAAAAGTTATCAAAACCCCTCGGTAGTTTTGGGGTAGGCTGGCCGGTCAGGTGGTAGTTCTACTACTA  >  NC_000913/1196089‑1196528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A