Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F113 I2 R1 19 51.3 1830242 91.7% 1678331 142.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,184,543 C→A P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,184,5430CA94.9% 109.9 / ‑5.7 41P1100Q (CCG→CAG) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (20/17);  total (24/17)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

GGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAA  >  NC_000913/4184406‑4184673
                                                                                                                                         |                                                                                                                                  
ggcccttACACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGCTAACAAGGGTGTAATTTCTAAGACCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTgg                                                                                                                               <  1:217384/138‑1 (MQ=255)
ggCGGGTAAGCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTgg                                                                                                                               <  2:832475/143‑1 (MQ=255)
   ggTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCCGTAGACACCGTACAGAACCGACGGGGCg                                                                                                                            >  2:455132/1‑143 (MQ=255)
        aaCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAAGCGGCGGGGCGtcgcg                                                                                                                       >  1:584368/1‑139 (MQ=255)
        aaCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGCAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTAATGAACGAGCTGGGCGTGCCg                                                                                                                       >  1:106443/1‑143 (MQ=255)
          cGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGcgcggaacgcc                                                                                                                     >  2:516589/1‑136 (MQ=255)
                            gACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACAGCGGTCa                                                                                                   >  1:395035/1‑143 (MQ=255)
                             aCAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAg                                                                                                  >  1:178088/1‑143 (MQ=255)
                                 gATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTCTGTACATCGTTCTGCTcc                                                                                              >  1:14545/1‑143 (MQ=255)
                                  attgCAGGTCGTCGCGGTAACAAGGGTGTAAGTTCTAAGATCACCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCt                                                                                             <  1:346898/140‑1 (MQ=255)
                                       aGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGCAAACGGTACGCCGGGAGACATCGTACTGAACCCGCTGGGCGTCCCGTCTCGTCTGACCACCGGACCGCTCCCCGaca                                                                                        >  2:232009/1‑141 (MQ=255)
                                               aCGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGCTCCTCGAAAccccccgg                                                                                >  1:883406/1‑141 (MQ=255)
                                                 ggTAACATGGGTGTAATTTCGAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTggg                                                                              <  2:474648/143‑1 (MQ=255)
                                                 ggTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTggg                                                                              <  1:912410/143‑1 (MQ=255)
                                                  gcaaCAAGGTTGTAATTTCTACGATCAACCCGATCGAAGATATGCCTTACGATGAAGACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCTGTTAGATCCTCGAAACCCACCTGGGt                                                                             <  2:903209/141‑1 (MQ=255)
                                                       aaGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCCGGGTCTGGc                                                                        >  2:633025/1‑143 (MQ=255)
                                                              tAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                 <  2:826333/143‑1 (MQ=255)
                                                                aTTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGCCCCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTCCGACAgg                                                               >  2:684894/1‑143 (MQ=255)
                                                                    ccaagcacAACCCGATCGAAGATAGGCCTTACGATGAAAACGGTACGCCGGTAGTCTGCCTACTATACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATc                                                           <  1:666229/136‑1 (MQ=255)
                                                                          tCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGAc                                                     >  1:191099/1‑143 (MQ=255)
                                                                            aaccgcaTCGACGAGATGCCGTACGATGGAAACGGTACGGCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACaa                                                   <  2:294856/137‑1 (MQ=255)
                                                                            aaCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACAGCGGTCAGATCCTCGCAACCCACCTGCGTATGGCTGCGAAAGGTATCGGCGACaa                                                   >  2:479079/1‑143 (MQ=255)
                                                                              cccGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGa                                                 >  1:737210/1‑143 (MQ=255)
                                                                               ccGATCGAAGAGATGCCTTACGATGAAAGCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCGCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGAt                                                <  2:106904/143‑1 (MQ=255)
                                                                                   tCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCGCACTGGGTATGGCTGCGAAAGGTAGCGGCGCCAAGATCCAc                                            >  2:662548/1‑143 (MQ=255)
                                                                                    cGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGCCACCCCCCTGGGTATGGCTGCGAAAGGTATCGGCCACAAGATCAACg                                           >  1:271254/1‑143 (MQ=255)
                                                                                     gAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACACGATAGACGc                                          >  1:242278/1‑143 (MQ=255)
                                                                                             gCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGTTCAGATCCTCGAAACCCACCTGGGGATGGCTGCGCAAGGTCTCCGCGACCAGATCAAAGCCATGCTGa                                  >  2:518076/1‑143 (MQ=255)
                                                                                             gCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGa                                  >  1:474983/1‑143 (MQ=255)
                                                                                               gttACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGaaa                                <  1:326553/142‑1 (MQ=255)
                                                                                                ttACGATGAAACCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAc                               <  1:598173/143‑1 (MQ=255)
                                                                                                  aCGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGACACCCACCTGGGTATGGCTGCGGCAGGTATCGGCGCCCAGGTCACCGCCATGCTGAAAcag                             >  1:37662/1‑143 (MQ=255)
                                                                                                  aCGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcag                             >  1:321966/1‑143 (MQ=255)
                                                                                                    gATGAAAACGGTCCGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTATCGTATGAACATCGGTCAGATCCTCGAAACCCACCGGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagca                           <  1:908775/143‑1 (MQ=255)
                                                                                                    gATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCCCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGCTGAACGCAATGCTGAAAcagca                           >  1:225848/1‑143 (MQ=255)
                                                                                                      tGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACagcagc                         <  1:490919/143‑1 (MQ=255)
                                                                                                        accaCGGTCCGCCGGTAGACATCGTACTGAACCAGCGGGGCTTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaa                       <  1:120606/140‑1 (MQ=255)
                                                                                                              gTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTc                 >  1:540931/1‑143 (MQ=255)
                                                                                                                  gCCGGTAGACATCGCACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGa             <  1:521128/143‑1 (MQ=255)
                                                                                                                            aTCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGCACATCGGTCAGATCCGCGAGACCCACCCGGCTATGCCTGCGACAGGTACCGGCGACCACATCAACCCCATGCCGCAGCAGCAGCCACCCGCCGCGAAAcaccccca   >  2:95938/1‑136 (MQ=255)
                                                                                                                             tCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGaa  <  1:893225/143‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                  
GGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAA  >  NC_000913/4184406‑4184673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A