Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F9 I0 R1	165	96.4	2178490	87.4%	1904000	226.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,058,486	T→C	12.2%	S135P (TCG→CCG)	torC →	trimethylamine N‑oxide (TMAO) reductase I, cytochrome c‑type subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,058,486	0	T	C	12.2%	261.5 / 2.7	117	S135P (TCG→CCG)	torC	trimethylamine N‑oxide (TMAO) reductase I, cytochrome c‑type subunit
Reads supporting (aligned to +/- strand): ref base T (64/31); new base C (4/14); total (68/49)
Fisher's exact test for biased strand distribution p-value = 4.96e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 117 total aligned reads displayed.

GTGTATGAAGAATATAAACAGTCGGTGCATTTCCAGAACGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTCATATCCCGC‑CGGATATTCCAGGCATGGTGAAGCGCAAACTGGAAGCGAG‑CAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCTAATGACAGTGGTGACACGCTGTACTCTATTGATAT  >  NC_000913/1058252‑1058715

gtgtATGAAGAATATAACCAGTCGGTGCATTTCCAGAACGCCTCCGGCGTGCGAGCTGAATGCCATAACTGTCATATCCCGC‑CGGATATTCCAGGCATGGTGAAGCGCAAACTGGAAGCGAG‑CAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AAATCGGc                                                                                                                                                                                                                                   >  1:569403/1‑239 (MQ=255)

gtgtATGAAGAATATAAACAGTCGGTGCATTTCCAGAACGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTCATATCCCGC‑CGGATATTCCAGGCATGGTGAAGCGCAAACTGGAAGCGAG‑CAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGCATGAAAGAAAAC‑AAGTCGGc                                                                                                                                                                                                                                   >  1:764789/1‑239 (MQ=255)

gtgtATGAAGAATATAAACAGTCGGTGCATTTCCAGAACGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTCATATCCCGC‑CGGATATTCCAGGCATGGTGAAGCGCAAACTGGAAGCGAG‑CAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAAAGAAAGAAAAA‑AACCCGGc                                                                                                                                                                                                                                   >  2:138311/1‑239 (MQ=255)

gtgtATGAAGAATATAAACAGTCGGTGCATTTCCAGAACGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTCATATCCCGC‑CGGATATTCCAGGCATGGTGAAGCGCAAACTGGAAGCGAG‑CAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTCCCG‑AGCGTGAATGGGCGCGAATGAAAAAAACC‑AACTCGGc                                                                                                                                                                                                                                   >  1:416769/1‑239 (MQ=255)

  gttataagaaTTAAACCAGTGGGTGCATTTCCAGACCGCCTCCGGGGTGCGAGCTTAATGCCATGACTGTCATATCCCGC‑CGGATTTTCCAGGCATGGTGAAGCGCAAACTGGAAGCGAG‑CAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCa                                                                                                                                                                                                                                  <  2:195660/233‑1 (MQ=255)

  gtATGAAGAATATAAACAGTCGGTGCATTTCCAGAACGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTCATATCCCGC‑CGGATATTCCAGGCATGGTGAAGCGCAAACTGGAAGCGAG‑CAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCa                                                                                                                                                                                                                                  <  1:994179/238‑1 (MQ=255)

              taAACAGTTTGTGCATTTCCAAACCGCCCTCGGCGGGCGGGCTGAATGCCATGACTGTCATACCCCGC‑CGGATCTTCCAGGCATGGGGAAGCGAAAACTGGAAGCGAG‑CAATGATATCTACCAGCCCTTTATTGCTCACTCCATGGATCCCCCTGAAAAATTAGAAGCCAACCGGG‑CGGAACTTGCCG‑AGCGTGAATGGGGGCGAATGAAAGAAAAC‑AACTCGGCACCCTGCCGCTCCt                                                                                                                                                                                                                     <  2:313677/239‑1 (MQ=255)

                    gTCGGTGCATTTCCAGAACGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTCATATCCCGC‑CGGATATTCCAGGCATGGTGAAGCGCAAACTGGAAGCGAG‑CAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATa                                                                                                                                                                                                               >  1:273272/1‑239 (MQ=255)

                    gTCGGTGCATTTCCAGAACGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTCATATCCCGC‑CGGATATTCCAGGCATGGTGAAGCGCAAACTGGAAGCGAG‑CAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAA‑AACTCGGCAACCTGCCCCCCCTGCCaaa                                                                                                                                                                                                               >  2:799002/1‑237 (MQ=255)

                    gTCGGTGCATTTCCAGAACGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTCATATCCCGC‑CGGATATTCCAGGCATGGTGAAGCGCAAACTGGAAGCGAG‑CAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAA‑AAATCGGCAACCCGCCGCCCCGGCCaaa                                                                                                                                                                                                               >  2:496922/1‑237 (MQ=255)

                     tCGGTGCATTTCCAGAACGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTCATATCCCGC‑CGGATATTCCAGGCATGGTGAAGCGCAAACTGGAAGCGAG‑CAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATaa                                                                                                                                                                                                              >  1:310318/1‑239 (MQ=255)

                     tCGGTGCATTTCCAGAACGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTCATATCCCGC‑CGGATATTCCAGGCATGGTGAAGCGCAAACTGGAAGCGAG‑CAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AAATCGGCAACCTGCCCCCCCCCCCATaa                                                                                                                                                                                                              >  2:1019458/1‑239 (MQ=255)

                     tCGGTGCATTTCCAGAACGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTCATATCCCGC‑CGGATATTCCAGGCATGGTGAAGCGCAAACTGGAAGCGAG‑CAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAAAAAAC‑AAACCGGCAAACTGCCGCCCCGGCCaaaa                                                                                                                                                                                                              >  2:381701/1‑239 (MQ=255)

                          gCATTTCCAGAACGCCCCCGGCGTGCGAGCTGAATGCCATGACTGTTAATCCCCGC‑CGGATATTCCAGGCATGGTGAAGCGCAAACTGGAAGCGAG‑CAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTAcg                                                                                                                                                                                                         <  1:999805/239‑1 (MQ=255)

                           cATTTCAAAAACGCCCCGGGCGGGCGAGCTGAATGCCATGACTGCCATATCCCGC‑CGGATATTCCAGGAATGGTGAAGCGCAAACTGGAAGCGAG‑CAATGATATCTACCAGCCCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTAcg                                                                                                                                                                                                         <  1:1034291/238‑1 (MQ=255)

                           agtttCCAGACCCCCTCCGGAGGTCGAGCTGAATGCCATGACGTGCATATCCCGC‑CGGAAATTCCAGGCATGGTGAAGCGAAAACTGGAAGCTAG‑CAAGGATATCTACAAGCCCTTTATTGCTCCTTCCATTGATACTCCTGAAAAATTCGAAGCCAACCGCG‑CGGAACTTACCG‑AGCGTGAAGGGTCGCAAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTAcg                                                                                                                                                                                                         <  1:532053/236‑1 (MQ=255)

                               tCCAGAACGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTCATATCCCGC‑CGGATATTCCAGGCATGGTGAAGCGCAAACTGGAAGCGAG‑CAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACTCG‑CGGAACTTGCCG‑AGCGGGAATGGGCGCGAATGAAAGAAAAA‑AACTCGGAAACCTGCCGCACCTGCCATaaaaacaaagag                                                                                                                                                                                                    >  2:571535/1‑229 (MQ=255)

                               tCCAGAACGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTCATATCCCGC‑CGGATATTCCAGGCATGGTGAAGCGCAAACTGGAAGCGAG‑CAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTAcgatggg                                                                                                                                                                                                    >  1:926903/1‑237 (MQ=255)

                                   gAACGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTCATATCCCGC‑CGGATATTCCAGGCATGGTGAAGCGCAAACTGGAAGCGAG‑CAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCCCCTGCCATAACTACCATGCGATgg                                                                                                                                                                                                >  1:472531/1‑239 (MQ=255)

                                    ggaatatCCGGCGTGCGAGCTGAATGCCATGACTGTCATATCCCGC‑CGGATATTCCAGGCATGGTGAAGCGCAAACTGGAAGCGAG‑CAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATgg                                                                                                                                                                                                >  1:73129/7‑238 (MQ=255)

                                     aCGCCGCGGGGGGGCGGGCTGAATGCCTTGCCTGGCATACCCCGC‑GGGATTTTCCAGGCATGGGGAAGCGCAAACGGAAAGCGAG‑AAATGATCTCTACCAACCCTTTTTTGCCCACCCCATGGATACCCCGAAAAATTTCGAAGCCAAACGCG‑GGGACCTTGCCG‑GGCGTGAATGGGGGCGAATGAAAGAAAAC‑AACTCGGCACCCTGCCGCTCCTGCCATAACTACGATGCGATGGa                                                                                                                                                                                               <  2:254656/238‑1 (MQ=255)

                                      cGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTCATATCCCGC‑CGGATATTCCAGGCATGGTGAAGCGCAAACTGGAAGCGAG‑CAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATc                                                                                                                                                                                             >  1:208437/1‑239 (MQ=255)

                                         cTCCGGCGTGCGAGCTGAATGCCATGACTGTCATATCCCGC‑CGGATATTCCAGGCATGGTGAAGCGCAAACTGGAAGCGAG‑CAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACCCGGCAACCTGCCGCTCCTGCCAAAACCACGATGCGAGGGAACaag                                                                                                                                                                                          >  2:1040840/1‑237 (MQ=255)

                                                 tGCGAGCTGAATCCCATGACTTCCATATCCCGC‑CGGATATTCCGGGCATGGTGAAGCGCAAACTGGAAGCGAG‑CAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAagca                                                                                                                                                                                   <  1:1019458/238‑1 (MQ=255)

                                                  gCGGGCTGAATGCCATGACTGTCATACCCCGC‑CGGATATTCCAGGCATGGTGAAGCGCAAACGGGAAGCGAG‑CAATGATATCTACCAGACCTTTATTGCTCCCTCCATTGATCCACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAagcag                                                                                                                                                                                  <  1:793267/238‑1 (MQ=255)

                                                         gAATGCCATGACTGTCATATCCCGC‑CGGATATTCCAGGCATGGTGAAGCGCAAACTGGAAGCGAG‑CAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATAATGCGAAGCAGCATCCTGa                                                                                                                                                                          >  1:668442/1‑239 (MQ=255)

                                                                       ttatatCCCGCGCGAATTTTCCAGGCTTGGTGAGCCGCAAACTGGAAGCGAA‑CAATGATTTCCACCAGCCCTTTTTTCCTCCCCCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGGGCGAATAAAAGAAAAC‑ACCTCGGCACCCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAg                                                                                                                                                             <  2:274228/237‑1 (MQ=255)

                                                                            tCCCGC‑CGGATATTCCAGGCATGGTGAAGCGCAAACTGGAAGCGAG‑CAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAg                                                                                                                                                       >  1:341489/1‑239 (MQ=255)

                                                                               cgc‑cgGATATTCCAGGCATGGTGAAGCGCAAACTGGAAGCGAG‑CAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAgggg                                                                                                                                                    >  1:266324/1‑237 (MQ=255)

                                                                                 c‑cgGATATTCCAGGCATGGTGAAGCGCAAACTGGAAGCGAG‑CAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAACCAGCATCCTGAACCAGCCCGCCCGATGAAGGGGGc                                                                                                                                                  >  2:72523/1‑239 (MQ=255)

                                                                                 c‑cgGATATTCCAGGCATGGTGAAGCGCAAACTGGAAGCGAG‑CAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGAAGGATCATGGGAAAAAGCACCCGGAAGAAGCACGTCAAAGGAAGgggga                                                                                                                                                  >  2:46559/1‑238 (MQ=255)

                                                                                                gtatgggAAGCCCAAACTGGGAGAGGGAAAATGAAATCTACCAGCCCTTTTTGGCTCACTCCATGGATACCCCTAAAAAATTCAAAGCCAAGCGAG‑GGGACCTTGCCG‑AGCTGGAATGGGCGCGAATGAAAGAAAAC‑ACCCCGGCACCCTGCCCCCCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATaat                                                                                                                                     <  2:54689/233‑1 (MQ=255)

                                                                                                ctgggTGAAGCCCAAACTGGAAGGGAG‑CAATGATATCTACCAGGCATTTATGGCCCACTCCATTGATACACCTGAAAAATTGGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATaat                                                                                                                                     <  1:46559/235‑1 (MQ=255)

                                                                                                aaTGGTAAAGCGCAAACTGGAAGCGAG‑CAATGATATCTACCAGTCCTTTATTGCTCACTCCATTGATACCCCGGAAAAATGCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATaat                                                                                                                                     <  1:799002/237‑1 (MQ=255)

                                                                                                 aTGGTGAAGCGCAAACTGGAAGCGAG‑CAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGAGGCAGCGAAAGATaatca                                                                                                                                   >  1:552993/1‑239 (MQ=255)

                                                                                                   ggTGAAGCGCAAACTGGAAGCGAG‑CAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATaatcaa                                                                                                                                  >  1:928463/1‑238 (MQ=255)

                                                                                                    gTGAAGCGCAAACTGGAAGCGAG‑CAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAACTTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCCGCACGTCAGATGAATGGGGCAGCGAAGGATaagcaat                                                                                                                                 >  1:687928/1‑238 (MQ=255)

                                                                                                    gTGAAGCGCAAACTGGAAGCGAG‑CAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCCCGTCAGATGAAGGTGGCAGGGAAAGATaatcaat                                                                                                                                 >  1:1022578/1‑238 (MQ=255)

                                                                                                             cacttGGAAGGGAA‑CAAGTATTTCTCCAAGTTCTTTTTTGCTCACTCCATTGATCCCCCTAAAAAATACGAAGCCAAACGCG‑CGGATCTTGCCG‑GGCGTGAAGGGGCGCGAATGAAAAAAACC‑ACCTCGGCACCCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGa                                                                                                                       <  2:588712/235‑1 (MQ=255)

                                                                                                             aaccgggAAGCGAG‑CAATGATACTTCCAAGCCCTTTATTGCCACCTCCATTGATACCCCGGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGa                                                                                                                       <  1:760817/234‑1 (MQ=255)

                                                                                                             aaccTGGAAGCGAG‑AAATTATATCTCCCAGCCCTTTATTGCT‑ACCCCATGAATACACCAAAAAATGTAGAAGCC‑AACGCGTCGAACCTCGCCG‑AGCGTGAATGGGGCCGAA‑GAAAAAAAACAAACCCGCCACCCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGa                                                                                                                       <  2:749862/235‑1 (MQ=255)

                                                                                                             aaaCGGAAAGCGAG‑AAAAGATATCACACAGCCCTTTATTGCCCACCCCATTGAAACCAGAAAAAAATTCGAAGCCAAACGCG‑GGGAACTTGCCG‑GGCGTGAAGGGCCGCGAAAGAAAGAAAAC‑AACCCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGa                                                                                                                       <  2:73129/239‑1 (MQ=255)

                                                                                                              actgggAAGCGAA‑AAATAATATTTTACAGCCTTTTATTGCTACCTCCTGTGATCAACCTAAAAAAACCGAAGCAAAACGCG‑CGGAAC‑TGCCGAAGCGTGAAGGGGCGCAAATGAAAAAAAAC‑ACCTCGGCAACCGGCCCCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGAc                                                                                                                      <  2:23854/235‑1 (MQ=255)

                                                                                                              accTGGAACCGGA‑CAAGAATAACTACCAGACCTTTATTGCCCACTCACTTGATACACCTGAAAAATTCGAAGCCAACGGCG‑CGGACCTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCACTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGAc                                                                                                                      <  1:1088284/237‑1 (MQ=255)

                                                                                                               ccTGGAAGGAAA‑CAAGGATACCTCCCAGCCCTTTATTGCTCAGTCAATGGATACACCTGAAAAATTCGAAGCAAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACt                                                                                                                     <  1:1040840/238‑1 (MQ=255)

                                                                                                               aCTGGAAGCGAG‑CAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCg                                                                                                                        >  1:1037070/1‑236 (MQ=255)

                                                                                                                     agagAG‑CATTGTTTTCTCCCAGCCTTTTATTGCCCACTCCTTTGATACCCCAGAAAAAGTCGAACACAAACGCG‑GGAAACTTGCGG‑GGGGTGAAGGGGTGCGAATGAAAGAAAAC‑AACCCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATa                                                                                                               <  2:266324/236‑1 (MQ=255)

                                                                                                                         aG‑CAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCACCTGTCATaaa                                                                                                             >  1:552518/1‑237 (MQ=255)

                                                                                                                                 atatCTACCAGACCTTTATTGCCCACTCCATTGATACCCCGGAAAAATTCGAAGCCAACCGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGcc                                                                                                    <  1:72523/239‑1 (MQ=255)

                                                                                                                                       aCCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGAACGTCAGATGAAGGGGGCACGGAAAGAAAATCAAACCTGCATCGACTGCAATAAAGGAATTGCCCACCAg                                                                                              >  2:851642/1‑239 (MQ=255)

                                                                                                                                         cAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAagcag                                                                                                                                                                                  >  2:791807/1‑153 (MQ=255)

                                                                                                                                         cAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACCCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAagcag                                                                                                                                                                                  <  1:791807/153‑1 (MQ=255)

                                                                                                                                          aGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCGGATGAAGGGGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGAGATTGCCCCCAAGtt                                                                                            >  2:148617/1‑238 (MQ=255)

                                                                                                                                           gACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGCTCATGCGAAGAAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTAc                                                                                          >  1:566079/1‑239 (MQ=255)

                                                                                                                                           gACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTa                                                                                           >  1:219229/1‑238 (MQ=255)

                                                                                                                                           gACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGGATTGCCCACCAGTTAc                                                                                          >  1:389970/1‑239 (MQ=255)

                                                                                                                                           gACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGAAGCATCCTGAACCAGCACGTCAGATGAAGGGGGCAGCGAAAGATAATCAATCCTGCATCAACTGCCATAAAGGAATTGCCCACAAGTAAc                                                                                          >  2:314548/1‑239 (MQ=255)

                                                                                                                                            aCCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGCACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATACTCAATCCTGCATCGACTGTCATAAAGGGATTTCCCACCCGTCAcc                                                                                         >  1:406987/1‑239 (MQ=255)

                                                                                                                                             ccTTTATTGCTCACTCCATGGATACCCCTGAAAAATTGAAAGCAAACCGCG‑CGGACCTTGCCG‑AGCGTGAAGGGGCGCGAATGAAAGAAAAC‑AACGCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTa                                                                                           <  1:496922/236‑1 (MQ=255)

                                                                                                                                                ttATTGCTCACTCCATTGATAAACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCACTCCTGCATCGACTGCCATAAAGGAATTGCCCACCAGTTACCGGat                                                                                     >  1:321206/1‑239 (MQ=255)

                                                                                                                                                      cTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGACTGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAACCAGCATCCTGAAACAGCACGCCAGATGAAGGGGGCAGCGAAAGATAAGAAATCCTGCATCGCCTGTCAAAAAGGAATTGCCCCCCAATAACCgggaaaagaa                                                                               >  2:558421/1‑231 (MQ=255)

                                                                                                                                                       tCACTCCATTGATACCCCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGa                                                                                                                                                                                               <  1:15597/126‑1 (MQ=255)

                                                                                                                                                       tCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGa                                                                                                                                                                                               >  2:15597/1‑126 (MQ=255)

                                                                                                                                                        cACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGCAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGCAATAACGGAATTGCCCACCGGTAACCGGaaataggaa                                                                              >  2:746492/1‑233 (MQ=255)

                                                                                                                                                        cACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGTTGGCAGCGAAAGATAATCCATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGaaatatgaaa                                                                             >  2:249660/1‑233 (MQ=255)

                                                                                                                                                        cACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGAAATGAGTa                                                                              >  1:779315/1‑238 (MQ=255)

                                                                                                                                                           tCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCCGCACGCCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATCAAGGTATTGCCCCCCAGTAACCGGAAATGAGTAGCgc                                                                          >  2:708084/1‑238 (MQ=255)

                                                                                                                                                           tCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAgg                                                                                                           >  1:565330/1‑206 (MQ=255)

                                                                                                                                                           cccATTGATCCCCCGAAAAAATCCGAGACAAAACGGC‑GGGAACTTGCCG‑AGCGGGAATGGGCGCGAAAGAAAGAAACC‑AACCCGGCAACCTGCCGCCCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCgg                                                                          <  1:708084/238‑1 (MQ=255)

                                                                                                                                                           ccaaTTGAAACCCCTAAAAAATGCGAAGCCAACCCGG‑GGGAACTTGCCG‑GGCGTAAAGGGGCGCAAATGAAAGAAAAC‑AACTCGGCAACCGGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCgg                                                                          <  1:138311/236‑1 (MQ=255)

                                                                                                                                                                tGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACGGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGACTTcc                                                                     >  1:993533/1‑239 (MQ=255)

                                                                                                                                                                 gATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGAGGAAGGTGGCAGCGAAAGATAATCAATCCTGAATCGCCTGCCATAAAGGTATTGCCCACCAGTTACCGGATATGAATAGGGGCCTCCg                                                                    >  2:121322/1‑239 (MQ=255)

                                                                                                                                                                   tACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGGGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGACTTTCCCACCAGTTACCGGATATGGGGAGCGGCTTCCGTa                                                                  >  2:832588/1‑239 (MQ=255)

                                                                                                                                                                   acccccaaaaaaaTTCGAAACAAAGCGCG‑CGAAACTTGCCG‑GGCGTGAAGGGGCGAGAATGAAAAAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTa                                                                  <  2:167355/231‑1 (MQ=255)

                                                                                                                                                                     cacCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCACCCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGAttacg                                                                                                                                                       >  1:701259/1‑148 (MQ=255)

                                                                                                                                                                     cacCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGCTCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCCGCGAGAGATACACAATCCTAACTCGCCTGCCATAAGGGCATTGCCCCCACGTTACCGGAAATGAGTAGGGGGTTCCGTaac                                                                >  2:1019888/1‑238 (MQ=255)

                                                                                                                                                                     cacCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGGGAACGATAATCAATCCTGCATCGACTGTCATAAGGGGATTGCCCAACAGTTACCGGATATGAGAAGGGGCTTCCGTaa                                                                 >  1:115268/1‑238 (MQ=255)

                                                                                                                                                                     cacCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAGGGAATTGCCCACCAGTTACCGGATATAAGTAGCGGCTTCCGTAAg                                                                >  1:182011/1‑239 (MQ=255)

                                                                                                                                                                     cacCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAg                                                                >  1:380061/1‑239 (MQ=255)

                                                                                                                                                                       ccTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCa                                                                                                                                                                                                                 >  2:754579/1‑92 (MQ=255)

                                                                                                                                                                       ccTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCCGTTACCGGATATGAGAAGCGGCTCCCGaaaaaa                                                              >  2:852128/1‑236 (MQ=255)

                                                                                                                                                                       ccTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCa                                                              >  1:448226/1‑239 (MQ=255)

                                                                                                                                                                       ccTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGGATTGCCCACCAGTTACCGGAAATGAGTAGCGGCTTCCGTAAgaa                                                              >  1:224119/1‑237 (MQ=255)

                                                                                                                                                                                ttCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATCATCAATCCTGCATGGACTGTCATAAAGGTATTGCCCCCCAGTTACCGGATATGAGTCGCGGCTTCCGAAAGCAGTTCGATGa                                                     >  2:293173/1‑239 (MQ=255)

                                                                                                                                                                                            acgcgggtactttGCGG‑GGCGTGAATGGGCGCAAATGAAAGAAAAC‑ACCTGGGCAACCTGCCGCCCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCa                                          <  1:207384/228‑1 (MQ=255)

                                                                                                                                                                                              gcggcgAACTTGCCG‑GGGGGGAATGGGCCGGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCCCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCa                                          <  1:234619/232‑1 (MQ=255)

                                                                                                                                                                                                                 gggAATGGGCGGGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCTAATGACAgtggtg                        <  1:148617/235‑1 (MQ=255)

                                                                                                                                                                                                                  tGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCTAATGACAGTGGTGac                      <  1:381701/238‑1 (MQ=255)

                                                                                                                                                                                                                     aTGGGCGCGAATGAAAGAAAAC‑AACCCGGCAACCTGCCCCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCTAATGACAGTGGTGACACg                   <  1:121322/238‑1 (MQ=255)

                                                                                                                                                                                                                     aTGGGCGAAAATGAAAAAAAAC‑ACCCCGGACACCTGCCGCCCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCTAATGACAGTGGTGACACg                   <  1:468843/238‑1 (MQ=255)

                                                                                                                                                                                                                       gggcgaaaagaaaaaaaaaC‑ACCGCGCCACCTCGCCCCCCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCTAATGACAGTGGTGACACGCt                 <  2:219229/227‑1 (MQ=255)

                                                                                                                                                                                                                        gggcaaaaTAAAAAAAAAC‑AACCCGGCAACCTGCCCCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCCCGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCTAATGACAGTGGTGACACGCt                 <  1:571535/231‑1 (MQ=255)

                                                                                                                                                                                                                          gcatgaataaagaaagC‑CACGCGCCACCCGCCCCCCCCTGCCATACCTCCAACACAGTGGATCAGGCGAAGCCGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCAAAAGATAATCAATCCTGCATCGACTGTCAGAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCTAATGACAGTGGTGACACTCTGTa              <  2:115268/230‑1 (MQ=255)

                                                                                                                                                                                                                          cgcgAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGTTATGAGTAGCGGCTTCCGTAAGCCGTTCGATGAGCTGCGCGCCAGTGCTGATGACAGTGGTGACACGcctt               >  1:1066479/1‑234 (MQ=255)

                                                                                                                                                                                                                          cgcgAATGAAAAAAAAC‑ACCCCGCCACCCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCTAATGACAGTGGTGACACGCTGTa              <  1:852128/238‑1 (MQ=255)

                                                                                                                                                                                                                          cgataaTGAAAAAAAAC‑AACCCGGAAACCTGCCGCTCCGGCCATAACCACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCTAATGACAGTGGTGACACGCTGTa              <  1:832588/234‑1 (MQ=255)

                                                                                                                                                                                                                           gagAATGAAAGAAAAC‑AACGCGGCAACCTGCCGCCCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCTAATGACAGTGGTGACACGCTGTAc             <  1:92269/236‑1 (MQ=255)

                                                                                                                                                                                                                               ataaaagaaaAC‑AACCCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCTAATGACAGTGGTGACACGCTGTACTCTAt        <  1:388331/236‑1 (MQ=255)

                                                                                                                                                                                                                               acaaaagaaaCC‑CCCTCCCCACCCTGCCTCCTCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCTAATGACAGTGGTGACACGCTGTACTCTAt        <  2:389970/236‑1 (MQ=255)

                                                                                                                                                                                                                                       aaaC‑CACCCGACACCCGGCCGCTCCGGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCTAATGACAGTGGTGACACGCTGTACTCTATTGatat  <  1:314548/237‑1 (MQ=255)

GTGTATGAAGAATATAAACAGTCGGTGCATTTCCAGAACGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTCATATCCCGC‑CGGATATTCCAGGCATGGTGAAGCGCAAACTGGAAGCGAG‑CAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATTCGAAGCCAAACGCG‑CGGAACTTGCCG‑AGCGTGAATGGGCGCGAATGAAAGAAAAC‑AACTCGGCAACCTGCCGCTCCTGCCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCTAATGACAGTGGTGACACGCTGTACTCTATTGATAT  >  NC_000913/1058252‑1058715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A