Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F17 I0 R1	92	90.7	2214160	82.3%	1822253	222.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,058,486	T→C	14.5%	S135P (TCG→CCG)	torC →	trimethylamine N‑oxide (TMAO) reductase I, cytochrome c‑type subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,058,486	0	T	C	14.5%	195.1 / 2.1	99	S135P (TCG→CCG)	torC	trimethylamine N‑oxide (TMAO) reductase I, cytochrome c‑type subunit
Reads supporting (aligned to +/- strand): ref base T (49/26); new base C (5/12); total (57/42)
Fisher's exact test for biased strand distribution p-value = 1.24e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGGTGTATGAAGAATATAAACAGTCGGTGCATTTCCAGAACGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTC‑ATAT‑CCCGCCGGATATTCCAGGCATGGTG‑AAGCGCAAACTGGAAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCTAATGACAGTGGTGACACGCTGTACTCTAT  >  NC_000913/1058249‑1058709

ccGGTGTATGAAGAATATAAACAGTCGGTGCATTTCCAGAACGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTC‑ATAT‑CCCGCCGGATATTCCAGGCATGGTG‑AAGCGCAAACTGGAAGCGAGCAATGATATCTACCAGACCTTTATTCCTCACTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACCC‑CCGGAACTTTCCGAGCGTGAA‑TTGGCGCG‑AATGAAAAAAAACA‑AATc                                                                                                                                                                                                                                   >  2:374848/1‑239 (MQ=255)

   gtgtATGAAGAATATAAACAGTCGGTGCATTTCCAGAACGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTC‑ATAT‑CCCGCCGGATATTCCAGGCATGGTG‑AAGCGCAAACTGGAAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑ATTGAAAGAAAACA‑ACTC‑gga                                                                                                                                                                                                                               >  1:632537/1‑238 (MQ=255)

   gtgtATGAAGAATATAAACAGTCGGTGCATTTCCAGAACGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTC‑ATAT‑CCCGCCGGATATTCCAGGCATGGTG‑AAGCGCAAACTGGAAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGc                                                                                                                                                                                                                               >  1:935356/1‑239 (MQ=255)

   gtgtATGAAGAATATAAACAGTCGGTGCATTTCCAGAACGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTC‑ATAT‑CCCGCCGGATATTCCAGGCATGGTG‑AAGCGCAAACTGGAAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGc                                                                                                                                                                                                                               >  1:178080/1‑239 (MQ=255)

   gtgtATGAAGAATATAAACAGTCGGTGCATTTCCAGAACGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTC‑ATAT‑CCCGCCGGATATTCCAGGCATGGTG‑AAGCGCAAACTGGAAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AAGGAAAGAAAACA‑ACAC‑GGc                                                                                                                                                                                                                               >  1:178841/1‑239 (MQ=255)

   gtgtATGAAGAATATAAACAGTCGGTGCATTTCCAGAACGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTC‑ATAT‑CCCGCCGGATATTCCAGGCATGGTG‑AAGCGCAAACTGGAAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGGGAA‑TGGGGCGG‑AAGAAAAGAAAAAA‑ACAC‑gga                                                                                                                                                                                                                               >  2:686179/1‑238 (MQ=255)

   gtgtATGAAGAATATAAACAGTCGGTGCATTTCCAGAACGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTC‑ATAT‑CCCGCCGGATATTCCAGGCATGGTG‑AAGCGCAAACTGGAAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGACCGTGAA‑TGGGCGCG‑AATGAAAGGAGACA‑ACTC‑GGc                                                                                                                                                                                                                               >  1:745031/1‑239 (MQ=255)

   gtgtATGAAGAATATAAACAGTCGGTGCATTTCCAGAACGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTC‑ATAT‑CCCGCCGGATATTCCAGGCATGGTG‑AAGCGCAAACTGGAAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATA‑CGAAGCCAAACGC‑GCGGAACTTGCCGTGCGTGAA‑TGGGCGCG‑ACTGAAAGCAAAAC‑ACCC‑GGc                                                                                                                                                                                                                               >  1:197371/1‑239 (MQ=255)

     gtATGAAGAATATAAACAGTCGGTGCATTTCCAGAACGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTC‑ATAT‑CCCGCCGGATATTCCAGGCATGGTG‑AAGCGCAAACTGGAAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCa                                                                                                                                                                                                                              >  1:849520/1‑238 (MQ=255)

          aagaaTATAAACGTGCGGGGCATTACCAGAACGCCTCGGGCGTGCGAGCTGAATGCCATGACTGTC‑ATAT‑CCCGCCGGATATTCCAGGCATGGTG‑AAGCGCAAACTGGAAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCGGAAAAATT‑CGAAGACAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGc                                                                                                                                                                                                                       <  1:728294/239‑1 (MQ=255)

          aagaaTATAAACAGCGGGTGCATTCCCAGAACGCCTCCGGTGTGCGAGCTGAATGCCATGACGGGC‑GTAC‑CCCGCCGGATATTCCAGGCATGGTG‑AAGCGCAAACTGGAAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAC‑CCTGc                                                                                                                                                                                                                       <  1:969361/239‑1 (MQ=255)

              atatACACAGTCGGTGCATTTCCAGAACGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTC‑ATAT‑CCCGCCGGATATTCCAGGCATGGTG‑AAGCGCAAACTGCAACCGAGCAATGATATCTACCAGACCTTTATTGCTCCCTCCATTGCTACCCCTGAAAAATT‑CGAAGCCAAACCC‑GCGCACCTTGCCGGTCGGGAA‑TGGGCCCG‑AAAGAAAAAAAAAA‑ACCC‑GCGAA‑CCCGcccc                                                                                                                                                                                                                    >  2:371482/1‑236 (MQ=255)

              atatAAACAGTCGGTGCATTTCCAGAACGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTC‑ATAT‑CCCGCCGGATATTCCAGGCATGGTG‑AAGCGCAAACTGGAAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGCTACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCCCG‑AATGAAAGAAAACA‑ACAC‑GGCAA‑CCcgccgc                                                                                                                                                                                                                    >  2:1061974/1‑238 (MQ=255)

              atatAAACAGTCGGTGCATTTCCAGAACGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTC‑ATAT‑CCCGCCGGATATTCCAGGCATGGTG‑AAGCGCAAACTGGAAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGc                                                                                                                                                                                                                    >  2:918933/1‑238 (MQ=255)

              atatAAACAGTCGGTGCATTTCCAGAACGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTC‑ATAT‑CCCGCCGGATATTCCAGGCATGGTG‑AAGCGCAAACTGGAAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACCC‑GCGGAACTTCCCGAGCGTGAA‑TGGGCGCG‑AATGAAAAAAAAAA‑ACCC‑AGCAA‑CCcgccgc                                                                                                                                                                                                                    >  2:989894/1‑238 (MQ=255)

              atatAAACAGTCGGTGCATTTCCAGAACGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTC‑ATAT‑CCCGCCGGATATTCCAGGCATGGTG‑AAGCGCAAACTGGAAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATAAACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGc                                                                                                                                                                                                                    >  1:943257/1‑238 (MQ=255)

                       gTCGGTGCATTTCCAGAACGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTC‑ATAT‑CCCGCCGGATATTCCAGGCATGGTG‑AAGCGCAAACTGGAAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATa                                                                                                                                                                                                         >  1:1065078/1‑239 (MQ=255)

                       gTCGGTGCATTTCCAGAACGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTC‑ATAT‑CCCGCCGGATATTCCAGGCATGGTG‑AAGCGCAAACTGGAAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATa                                                                                                                                                                                                         >  1:447168/1‑239 (MQ=255)

                       gTCGGTGCATTTCCAGAACGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTC‑ATAT‑CCCGCCGGATATTCCAGGCATGGTG‑AAGCGCAAACTGGAAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGGGAA‑TGGGCGCG‑AATGTAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATa                                                                                                                                                                                                         >  1:719062/1‑239 (MQ=255)

                        tCGGTGCATTTCCAGAACGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTC‑ATAT‑CCCGCCGGATATTCCAGGCATGGTG‑AAGCGCAAACTGGAAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATa                                                                                                                                                                                                         >  1:726453/1‑238 (MQ=255)

                        tCGGTGCATTTCCAGAACGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTC‑ATAT‑CCCGCCGGATATTCCAGGCATGGTG‑AAGCGCAAACTGGAAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGAAA‑CCTGCCCCCCCTC‑CCATa                                                                                                                                                                                                         >  1:735270/1‑238 (MQ=255)

                         cGGTGCATTTCCAGAACGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTC‑ATAT‑CCCGCCGGATATTCCAGGCATGGTG‑AAGCGCAAACTGGAAGCTAGCAATGAAATCTACCAGGCCTTTATTGCTCACTCCATTGATAAAACTGACAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAAAA‑ACCC‑GGCAA‑CCTGCCCCCCCTT‑CCATAAc                                                                                                                                                                                                       >  1:860671/1‑239 (MQ=255)

                         cGGTGAATTTCCAGAACGCCCCCGGGGTGCGAGCTGAATGCCACGGCGGTA‑ATCC‑CCCGCCGGATATTCCAGGCATGGTG‑AACCGCAAACTGGAAGCGAGCAATGATATCTACCAGACTTTTATTGCTCACTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGGGCGTGAA‑TGGGGGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATAAc                                                                                                                                                                                                       <  2:124265/239‑1 (MQ=255)

                             tcttttCCCGACCCCCCCCGGCGGGGGAGGGAAATGCCA‑GTATGTCAATAC‑CCCCCGGGATTTTCCAGGCA‑GGTGAAAGCGCAACCTGGAAGCGCACATTGATATCTCCCAGCCCTTTAGTGCTCACTCCATTGATACCCCTGAAAAATT‑CAACGCCAAACGC‑GCGGAACTTCCCGAGCGTGAA‑TGGGCGCG‑AATAAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTAcg                                                                                                                                                                                                   <  2:648305/236‑1 (MQ=255)

                              cttttCCAAGACGCCTCCGGGTGGCGAGCTGAATGCCATGACTGTC‑ATAC‑CCCGCCGGATATTCCAGGCATGGTG‑AAGCGCAAACTGGAAGCGAGCAATGATATCGACCAGCCCTTTATTGCTCACTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTAcg                                                                                                                                                                                                   <  1:857976/236‑1 (MQ=255)

                                 ttCCAGAACGCCTCCGGCGTGCGGTCTGAATGCCATGACTGTC‑AGAT‑CCCGCCGGATATTCCAGTCATGGTA‑AAGCGAAAACTGGAATCGAGCAATGATACCTACCAGACCTTTATTGCTCTCTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAAAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTAcgatgc                                                                                                                                                                                               <  1:626891/239‑1 (MQ=255)

                                    cAGACCGCCCCCGGCGTGCGAGCTGAATGCCATGACTGTC‑ATAT‑CCCGCCGGATATTCCAGGCATGGTG‑AAGCGCAAACTGGAAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACCCCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTAcgatgcgat                                                                                                                                                                                            <  1:374848/239‑1 (MQ=255)

                                      gAACGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTC‑ATAT‑CCCGCCGGATATTCCAGGCATGGTG‑AAGCGCAAACTGGAAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCAATTGATAAACCTGAAAAATT‑CGAAGCCAACCGC‑GCGGACCTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAAAAAACA‑ACCC‑GGCAA‑CCTGCCGCCCCTG‑CCAACACACcgaggcgatga                                                                                                                                                                                          >  2:194163/1‑238 (MQ=255)

                                       aaCGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTC‑ATAC‑CCCGCCGGATATTCCAGGCATGGTG‑AAGCGCAAACTGGAAGCGAGCAATGATATCTACCAGCCCTTTATTGCTCACTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTACGATGCGATGGa                                                                                                                                                                                         <  1:686179/239‑1 (MQ=255)

                                              ccGGGGTGCGGACTGATTGCAATGGCTTGC‑ATCC‑CCCGCCGAATTTCCCAGGCATGGTA‑AAGCGAAAACTGGAAGCGAGCAATTATATCTCCCAGACTTTTATTGCCCACTCCATTGATACACCTGAAAAATG‑CAAAGCCAAACGC‑GCGGAACTGGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAAAA‑ACCC‑GGCAA‑CCGGCCGCTCCTG‑CCATAACTACGATGCGATGGATCATGCg                                                                                                                                                                                  <  2:1065078/239‑1 (MQ=255)

                                              ccGGCGTGCGAGCTGAATGCCATGACTGTC‑ATAT‑CCCGCCGGATATTCCAGGCATGGTG‑AAGCGCAAACTGGAAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACAC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAAAAAACA‑ACTC‑GGCAC‑CCTGCCGCCCCTG‑CCATAACTCACATGGAATGGATAATggg                                                                                                                                                                                  >  2:643687/1‑237 (MQ=255)

                                                  ggTGCGAGCTGAAGGCCATGACTGTC‑‑TATCCCCGCGGGATTTCCCAGGCATGGTG‑AAGCGCAAACTGGAAGCGAGAAATGATAGCTCCCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTACGATGCGATGGATCATGCGAag                                                                                                                                                                               <  1:367955/237‑1 (MQ=255)

                                                    tGCGAGCTGAATGCCATGACTGTC‑ATAT‑CCCGCCGGATATTCCAGGCATGGTG‑AAGCGCAAACTGGAAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCCTTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑CCCGAACTTGCCGAGCGTGAA‑TGGCCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTC‑CCATAACTACAAAGCGATGAGTCATGCGagcag                                                                                                                                                                             >  1:404162/1‑234 (MQ=255)

                                                      cGAGCTGAATGCCATGACTGTC‑ATAT‑CCCGCCGGATATTCCAGGCATGGTG‑AAGCGCAAACTGGAAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTACGATGCGATGGATCATGCGAAgcagca                                                                                                                                                                          >  1:993044/1‑239 (MQ=255)

                                                              aTGCCATGACTGTC‑ATAT‑CCCGCCGGATATTCCAGGCATGGTG‑AAGCGCAAACTGGAAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑TCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCGTCCGCTCCGG‑CCATAACTACGAAGCGAGAGATCATGCGAAGAAAAAGCCTGaac                                                                                                                                                                  >  2:222591/1‑238 (MQ=255)

                                                                    tGACTGTC‑ATAT‑CCAGCCGGATATTCCAGGCATGGTG‑AAGCGCAAACTGGAAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGCGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTACAATGCGATGGATCATGCGCAGCCGAATCCTGAagaagcc                                                                                                                                                             >  1:585096/1‑237 (MQ=255)

                                                                       cTGTC‑ATAG‑CCCGCCGAATATGCCAGGCATGGTG‑AAGCGAAAACTGGAATCGAGCAATGATATCTACCAGCCCTTTATGGCTCACTCCATTGATACGCCTGAAAAATG‑CGAAGCCAAACGC‑GCGGAACTTGCCGGGCGTGAA‑TGGGCGCA‑AATGAAAGAAAACA‑ACTC‑GGAAA‑CCTGCCGCTCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGt                                                                                                                                                          <  1:38034/238‑1 (MQ=255)

                                                                          tgtaatcccccGCCGGATATCCCAGGCATGGTG‑AAGCGCAACGGGGAAGCGAAAAATTAAAACCACCAGACCTTAATGGCTCACGCCATGAATCCACCTGAAAAATT‑GGAAGCAAAACGC‑GGGGATCTTGCCGGGCGAGAA‑TGGGCGCA‑AAGGAAAGAAAACA‑ACCC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCa                                                                                                                                                        <  2:802037/231‑1 (MQ=255)

                                                                                      ccgGATATTCCAGGCATGGTG‑AAGCGCAACCTGGAAGCGAGCAATGATATCTACCCGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAC‑TGGGCGCG‑AATGAAAGAACACA‑ACTC‑GGCAA‑CCGGCCG‑TCCTGCCCCTACCTACGATGCGAAGGATCATGCAGACAACCCTCCGGAACCAACACGCCCAAGGAAGGTGGc                                                                                                                                            >  2:514996/1‑239 (MQ=255)

                                                                                      ccgGATATTCCAGGCATGGTG‑AAGCGCAAACTGGAAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTACGATGCGATGGATAATGGGAAGCAGCATCCGGAACCAGCACGTCAGATGAAGGTgg                                                                                                                                             >  1:249530/1‑238 (MQ=255)

                                                                                                   gCATGGTG‑AAGCGCAAACTGGAAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGCTGAAGGTGGCAGCGAAAGATaat                                                                                                                               >  1:496456/1‑239 (MQ=255)

                                                                                                     tgttgataaGCGAAAACGGAAAGCGAAAAATTATACCCACCAGACCTTTATTGCCCGTCCCATTAATACCACTGAAAAATC‑CGAAGCAAACCGA‑GCGGAACGTCCCGAGTGTGAA‑TGGGCGCA‑AATGAAAGAAACCA‑CCCC‑GGCAC‑CCTGCCGCTCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATaat                                                                                                                               <  2:69516/231‑1 (MQ=255)

                                                                                                        gTG‑AAGCGCAAACTGGAAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAACAATT‑CGAAGCCAAACAC‑GCGGAACTTGCCGAGCGTGAA‑TGGCCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑ACTGCCGCGCCTG‑CCATAACTACGATGCGATGGATAATGCGAAGAAGCAACCTGAACACGACCCTCAGAGGAAGGGGGCAGAGaaaaaaaaaaaagc                                                                                                                          >  2:295047/1‑228 (MQ=255)

                                                                                                        gTG‑AAGCGCAAACTGGAAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCCTAACTACGATGCGATGGATACTGCGAAGCAGCATCCTGAAGCCACCCGTCAGACTAAGGTGGCAGCCAAAAATaaacaaa                                                                                                                           >  2:667033/1‑237 (MQ=255)

                                                                                                                   accgggAAGAGAGACATAATTTCCACCGAACCTTTCTTGCTCACTCCATTGATACCCGTAAAAAGTT‑CGAAGCAAAACGC‑GCGGAATTTGCCGGGCGTAAA‑GGGGCGAA‑AATGAAAAAAAAAA‑ACGC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGAc                                                                                                                <  2:632537/235‑1 (MQ=255)

                                                                                                                   aaCTGGAAGCGGACACTGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATT‑CGACTCCAAACCC‑GCGGAACTTGCCGTGCGTGAA‑TGGTCGCC‑ATTGAAAGCAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATACCTACGATGCGAAGACTCATGCAGGCCAGCATCCTGCAGCACACCGTCAAATCAAGGTGGCAGCGCAAGCAAACAAATACTGCacaaat                                                                                                                >  2:600773/1‑234 (MQ=255)

                                                                                                                   aaCTGGAAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTACGCTGCAATGGATAATGCGAAGAGACATCCTGAACAAACACGTCAGCTGAAGGTGCCAGCGAAAAAAAACCAACCCTCCACCggc                                                                                                                >  2:1065429/1‑237 (MQ=255)

                                                                                                                   aaCTGGAAGCGAACAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGAc                                                                                                                <  1:918933/239‑1 (MQ=255)

                                                                                                                   aaCGGGAAGGGAGAAAGGAAATCTACCAGCCCTTTATTGCTCACCCCATTGATACCCCTGAAAAATG‑CGAAGCAAAACGC‑GCGGAACTTGCCGAGGGTGAA‑TGGGCGCG‑AAGAAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGAc                                                                                                                <  1:667033/239‑1 (MQ=255)

                                                                                                                      tGGAAGCGAGCAATGATATCTCCCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAC‑CCTGCCGCTCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGt                                                                                                             <  1:1061974/239‑1 (MQ=255)

                                                                                                                                        tCTACCAGACCTTTATTACTCACTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCg                                                                                                                  >  1:854495/1‑216 (MQ=255)

                                                                                                                                           tccAGACCTTTATTGCTCACTCAATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTAcgatgcg                                                                                                                                                                                              <  1:742632/136‑1 (MQ=255)

                                                                                                                                           aCCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTAcgatgcg                                                                                                                                                                                              >  2:742632/1‑137 (MQ=255)

                                                                                                                                               gACCTTTATGGCCCACTCAATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGAAACTGGCCGAGGGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCGGCCGCTCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTAc                                                                                    <  1:371482/239‑1 (MQ=255)

                                                                                                                                                 ccTTTATTGCCCACTCCATTGAACCCCATGAAAAACG‑CGAAGCCAAACGC‑GCGGAACTTCCCGGGCGTGAA‑TGGGCGCG‑AATGAAAAAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCg                                                                                  <  1:1065429/239‑1 (MQ=255)

                                                                                                                                                  cTTTATTGCTCACTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGGGGCAGCGAAAGAGAATCAACCCTGCATCGACTGTAATAAAGGAATTGCCCCCCAGGTACCgg                                                                                 >  2:768631/1‑239 (MQ=255)

                                                                                                                                                    ttATTGCTCACTCCATTGATACACCTGAAAACTT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAATCAGCACGTCAGATGAAGGTGGCAGCGAAGGATAATCAATCCTGCATCGCCTTCCATAAAGGAATTGCCCACCAATTACCgaaa                                                                               >  2:597225/1‑236 (MQ=255)

                                                                                                                                                    ttATTGCTCACTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACt                                                                                                                                                                                                      >  1:476345/1‑120 (MQ=255)

                                                                                                                                                    ttATTGCTCACTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCCCGTCAGATGAAGGTGGCAGCGAAAGATACTCAATCCTGCACCGACTGTCCAAAAGGAATTCCCCACCAGTTACCGGaa                                                                               >  2:244002/1‑238 (MQ=255)

                                                                                                                                                    ttATTGCTCACTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGat                                                                               >  1:83490/1‑239 (MQ=255)

                                                                                                                                                    ttATTGCTCACTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGAATTGCCCACCAGTTACCGGat                                                                               >  1:623431/1‑239 (MQ=255)

                                                                                                                                                            cACTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCccacca                                                                                         >  1:775905/1‑221 (MQ=255)

                                                                                                                                                            cACTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAg                                                                       >  1:660707/1‑239 (MQ=255)

                                                                                                                                                               tCCATTGATACCCCTAAAAAATT‑GGATGCCAAACGC‑GGGGAACTTGCCGAGCGTAAA‑TGGGCGCG‑AATGAAAGAAAACA‑CCTC‑GGCAA‑CCGGCCGCTCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCgg                                                                    <  1:194163/239‑1 (MQ=255)

                                                                                                                                                               tCCATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGAt                                                                                                                                                     >  1:743131/1‑158 (MQ=255)

                                                                                                                                                               tCCATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGGAAGATAATCAATCCTGCATCGACTGTCATAAAg                                                                                                      >  1:88963/1‑205 (MQ=255)

                                                                                                                                                                 cgttGATACCCCAGAAAAATG‑CGCACCAAACCGC‑GGGAACCCTGCGGGGGGTGAC‑GGGGCGGA‑AATGAAAGAACAAA‑CCCC‑GGCAA‑CGTGCCGCCCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCt                                                                  <  2:660707/237‑1 (MQ=255)

                                                                                                                                                                    tGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGCATTGCCCACCCGTTACCGGCTATGAGTAGCGGCTTc                                                                >  2:125231/1‑238 (MQ=255)

                                                                                                                                                                      aTACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTACGATGCGCTGGATCATGCGAAGCAGCATCCTAAAGCAGCCCGTCAGATGAAGGTGGTAGCGCAAGATAATCAATCCTGCATCGCCTGTCAAAAAGGTAGTGCCCACCAATTCCCGGATATAAGTAGCGGcccacaa                                                             >  2:586771/1‑233 (MQ=255)

                                                                                                                                                                         ccccTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTACTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGGGGCAGCGTAAGAAAATCAATCCTGCACCGACTGTCATAAAGGGATTGCCCACCAGTTACCGGATATgtg                                                                          >  1:348753/3‑221 (MQ=255)

                                                                                                                                                                         cacCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTAcgatgcgat                                                                                                                                                                                            <  1:751282/109‑1 (MQ=255)

                                                                                                                                                                         cacCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTAcgatgcgat                                                                                                                                                                                            >  2:751282/1‑109 (MQ=255)

                                                                                                                                                                         cacCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAg                                                          >  1:796277/1‑239 (MQ=255)

                                                                                                                                                                         cacCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGAATTGCCCACCAGTTCCCGGATATGAGTAGCGGCTTCCGTAAg                                                          >  1:122358/1‑239 (MQ=255)

                                                                                                                                                                           ccTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCCCGTCAGATGAAGGTGGAAGAGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGAATTGCCAACAAGTTACCGGAAATGAGGAGCGGCTTCCTTaacaa                                                        >  2:9645/1‑236 (MQ=255)

                                                                                                                                                                           ccTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGAATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCa                                                        >  1:202463/1‑239 (MQ=255)

                                                                                                                                                                           ccTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTAAGATAAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGGATTGCCCACCAGTTACCGGAAATGAGTAGGGGCTCCCgacaaga                                                        >  2:687077/1‑233 (MQ=255)

                                                                                                                                                                           acTGAAAAATC‑CGAAGCAAAACGC‑GGGGAACTGCCCGGGCGTAAA‑GGGGCGCG‑AATAAAAAAAAACA‑ACCC‑GGCAA‑CCGGCCGCTCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCa                                                        <  1:620533/238‑1 (MQ=255)

                                                                                                                                                                            ccgAAAAATTCCGAAACAAAACGC‑GCGACAC‑TGCCGGGGGGGAA‑TGGGGCCGAAATGAAAAAAAACA‑ACGC‑GGAAA‑CCTGCCGCTCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCa                                                        <  2:935356/237‑1 (MQ=255)

                                                                                                                                                                            cTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGCCTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAg                                                       >  1:100383/1‑239 (MQ=255)

                                                                                                                                                                             tGAAAAATT‑CGAAGCAAAACGG‑GCGGAACTTGCCGAGCGGGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACCC‑GGCAA‑CCGGCCGCTCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGt                                                      <  1:1094006/239‑1 (MQ=255)

                                                                                                                                                                                   aTT‑CGAACCCAAACGC‑GAGGAACTTGCCGGGCGGGAA‑GGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCCCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATg                                                <  1:643687/239‑1 (MQ=255)

                                                                                                                                                                                          acccAACCGC‑GGGGAACTTGCCGGCTGGGAAGTGGGAG‑T‑AATGAAAGAAAACA‑CCTG‑GGCAA‑CCGGCCGCTCCTG‑CCAAAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCAAGTCAGATGAAGGTTGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTg                                           <  2:447168/236‑1 (MQ=255)

                                                                                                                                                                                           accAAACGCGGGGGACCTTGCCGAGGGTGAA‑TGGGCGCA‑AAGAAAAGAAAACA‑ACCC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTg                                           <  1:687077/237‑1 (MQ=255)

                                                                                                                                                                                              aaaCGC‑GGGGAGCTTGCCGGGCGGAAA‑TGGGCGCG‑AATAAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTgcgcgc                                      <  1:244002/239‑1 (MQ=255)

                                                                                                                                                                                                aCGC‑GCGAAATGTGCCGAGCGGGAA‑TGGGGGCA‑AATGAAAGAAAACA‑CCTG‑GACAC‑CCTCCCGATCCTG‑CCACAACTACGGTGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCa                                    <  1:514996/239‑1 (MQ=255)

                                                                                                                                                                                                           gttGCCGGGCGTGAA‑TGGGCG‑T‑AAAAAAAAAAAACACACTG‑GGAAC‑CCGCCCGCCCCGG‑CCTTAACTACGATGCGAGGGGTCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCCCCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCTAATGa                          <  2:820743/238‑1 (MQ=255)

                                                                                                                                                                                                            ttGGCGGGCGGGAA‑TGGGGGGC‑AAAGAAAAAAAAAA‑ACCCGGGCAA‑CCGCCCCCTCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCTAATGa                          <  2:83490/239‑1 (MQ=255)

                                                                                                                                                                                                                   gCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCTTG‑CCATAACTACGATGCGATGGATCATGCGCAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTCCCGTAAGCAGTTCGATGAGCTGCCCGCCAGTGCTAATGACAggcgga                  >  2:1065521/1‑234 (MQ=255)

                                                                                                                                                                                                                   gCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTACCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCAAGGGCTAAtacaagggggg                  >  1:531105/1‑229 (MQ=255)

                                                                                                                                                                                                                     ggtaaggggggGCG‑AATAAAAAAAAACA‑ACTG‑GGAAA‑CCTCGCGCCCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCTAATGACAgtggtg                  <  2:623431/231‑1 (MQ=255)

                                                                                                                                                                                                                      tGAA‑GGGGCGAA‑AATGAAAGAAAAAC‑ACCC‑GGCAA‑CCTGCCGCCCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCTAATGACAGTGGTGac                <  1:597225/238‑1 (MQ=255)

                                                                                                                                                                                                                         a‑TGGGCGCA‑AATGAAAGAAAACA‑ACCC‑GGCAC‑CCTGCCGCTCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCTAATGACAGTGGTGACACg             <  1:768631/238‑1 (MQ=255)

                                                                                                                                                                                                                           ggggggag‑AATAAAAAAAAACA‑CCCC‑GGCAC‑CCGCCCGCCCCTG‑CCATAACTACGATGGGATGGATCATGCGAAGCAGCATCCTGAAGCAGCAAGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCTAATGACAGTGGTGACACGCt           <  2:6612/232‑1 (MQ=255)

                                                                                                                                                                                                                              gccgaaaaaaaaaaaaaaCC‑AC‑C‑GGCAACCCTGCCCCCCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCTAATGACAGTGGTGACACGCt           <  2:202463/227‑1 (MQ=255)

                                                                                                                                                                                                                               cgca‑AATGAAAAAAACCA‑CCCC‑GGAAA‑CCTGCCGCTCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCTAATGACAGTGGTGACACGCTGTa        <  1:596804/238‑1 (MQ=255)

                                                                                                                                                                                                                                cgacaaTAAAAGAAACAA‑CCGC‑GGCAC‑CCTGCCCCTCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCTAATGACAGTGGTGACACGCTGTa        <  1:295047/234‑1 (MQ=255)

                                                                                                                                                                                                                                     aggaaataaaCCA‑ACTC‑GGCCA‑CCTGCCGCCCCTG‑CCAAACCTACGATGCAATGGATCATGGGAAGCAGCATCGTGAAGCAGCACGTCAGAGGAAGGTGGCAGCGAAAGATAAGCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTTCGCGCCAGTGCTAATGACAGTGGTGACACGCTGTACTCTAt  <  2:719062/237‑1 (MQ=255)

                                                                                                                                                                                                                                     aTGAAAAGAAACA‑ACTC‑GGCCA‑CCTGCCGCTCCTG‑CCATAACTTCGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCTAATGACAGTGGTGACACGCTGTACTCTa   <  1:1106770/238‑1 (MQ=255)

                                                                                                                                                                                                                                                     tC‑GGCAA‑CCTGCCGCTCCTT‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATaatcaatc                                                                                                                          >  1:827527/1‑104 (MQ=255)

CCGGTGTATGAAGAATATAAACAGTCGGTGCATTTCCAGAACGCCTCCGGCGTGCGAGCTGAATGCCATGACTGTC‑ATAT‑CCCGCCGGATATTCCAGGCATGGTG‑AAGCGCAAACTGGAAGCGAGCAATGATATCTACCAGACCTTTATTGCTCACTCCATTGATACACCTGAAAAATT‑CGAAGCCAAACGC‑GCGGAACTTGCCGAGCGTGAA‑TGGGCGCG‑AATGAAAGAAAACA‑ACTC‑GGCAA‑CCTGCCGCTCCTG‑CCATAACTACGATGCGATGGATCATGCGAAGCAGCATCCTGAAGCAGCACGTCAGATGAAGGTGGCAGCGAAAGATAATCAATCCTGCATCGACTGTCATAAAGGTATTGCCCACCAGTTACCGGATATGAGTAGCGGCTTCCGTAAGCAGTTCGATGAGCTGCGCGCCAGTGCTAATGACAGTGGTGACACGCTGTACTCTAT  >  NC_000913/1058249‑1058709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A