Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A69 F2000 I3 R1 36 84.1 1889666 90.2% 1704478 220.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007779_cfb 2,409,829 C→A intergenic (‑87/+544) nuoA ← / ← lrhA NADH:ubiquinone oxidoreductase, membrane subunitA/DNA‑binding transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_007779_cfb2,409,8290CA97.4% 240.3 / ‑1.7 78intergenic (‑87/+544)nuoA/lrhANADH:ubiquinone oxidoreductase, membrane subunitA/DNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (40/36);  total (41/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.87e-01

CGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTT  >  NC_007779_cfb/2409616‑2410069
                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                
cgcgTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTaa                                                                                                                                                                                                              >  2:762853/1‑250 (MQ=255)
        cgACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGAtgtg                                                                                                                                                                                                      >  1:73169/1‑250 (MQ=255)
          aCCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGt                                                                                                                                                                                                    >  1:558270/1‑250 (MQ=255)
          aCCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGt                                                                                                                                                                                                    <  2:230057/250‑1 (MQ=255)
              ccTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGttttt                                                                                                                                                                                                <  1:913354/250‑1 (MQ=255)
                 aaaaaCCAACCGCCTACCAGCATCAGGCAACACAGTGCAATGGCAACGATAAGAAAGATAGCGAAAGCCCAGTGATGAGCGAGGACTTCAGTGGATGTTGTCATACTCATTGCTTACCCATCAAAAGAAGCGTCAGATTCTGTGATCTTCACGGCAGATGGACGCCACATCGATTAATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATATAACTACTTGATGTGGTTGTTTAc                                                                                                                                                                                             >  1:624421/1‑250 (MQ=255)
                             ccTACCAGCATTAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACa                                                                                                                                                                                                                   <  2:559600/216‑1 (MQ=255)
                             ccTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACa                                                                                                                                                                                                                   >  1:559600/1‑216 (MQ=255)
                                     cATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCACCAGATTCACTGCTCTTCACGGCAGATGGACTCCACATCGATTCATGGGGATGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGt                                                                                                                                                                         <  2:413388/250‑1 (MQ=255)
                                             aaCACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAAt                                                                                                                                                                                                             >  2:374284/1‑206 (MQ=255)
                                             aaCACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAAt                                                                                                                                                                                                             <  1:374284/206‑1 (MQ=255)
                                                    gCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATTAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAAc                                                                                                                                                                     <  2:401693/239‑1 (MQ=255)
                                                    gCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATTAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAAc                                                                                                                                                                     >  1:401693/1‑239 (MQ=255)
                                                     acAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTTGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACcaaact                                                                                                                                                         >  2:722205/2‑248 (MQ=255)
                                                      cAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGtt                                                                                                                                                        >  1:915499/1‑250 (MQ=255)
                                                      cAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGCGGATGTTGACATACTCATTGCTTACTCATCAAAAGCAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAACAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGtt                                                                                                                                                        >  1:881754/1‑250 (MQ=255)
                                                         tGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGAGTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTttct                                                                                                                                                     <  2:73169/250‑1 (MQ=255)
                                                         tGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCAACAAAAGTAGCGCCAGATTCACTGCTCTAAACGGCAGATGGACGCCACATCGATTCAGGGGGAGGAATAAAAAAAAACTTACAATCACTGTACAAATTCTTTTATACAGCTAATTGATGTGGTGTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTtgct                                                                                                                                                     <  2:179311/250‑1 (MQ=255)
                                                          ggCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTttctt                                                                                                                                                    >  1:909885/1‑250 (MQ=255)
                                                                  tAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCTCTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAg                                                                                                                                            >  1:20091/1‑250 (MQ=255)
                                                                  tAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACa                                                                                                                                                                                                                   <  1:295159/179‑1 (MQ=255)
                                                                  tAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGCGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACa                                                                                                                                                                                                                   >  2:295159/1‑179 (MQ=255)
                                                                    agaaagaTAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGtt                                                                                                                                          >  1:907847/1‑250 (MQ=255)
                                                                     gaaagaTAGCGAATGCCCAGTGATGAGCGATGATTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTATAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGttt                                                                                                                                         >  1:840354/1‑250 (MQ=255)
                                                                        agaTAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTAATGGGGAGGAATAAAAAAAAACTTACAATCa                                                                                                                                                                                                                                        <  1:644602/152‑1 (MQ=255)
                                                                        agaTAGCGAATGACCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCa                                                                                                                                                                                                                                        >  2:644602/1‑152 (MQ=255)
                                                                                aaTGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTTATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGCGAGGAATAAAAAAAAACTTACAATCACTGTAGa                                                                                                                                                                                                                                 >  2:557181/1‑151 (MQ=255)
                                                                                aaTGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGTGAGGAATAAAAAAAAACTTACAATCACTGTAGa                                                                                                                                                                                                                                 <  1:557181/151‑1 (MQ=255)
                                                                                  tGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAac                                                                                                                            >  2:244486/1‑250 (MQ=255)
                                                                                      gaGNGNNGAGCGATGACGTCAGTGGATGTTGACATACTGATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCa                                                                                                                        <  2:640870/249‑1 (MQ=255)
                                                                                           atgaGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCtat                                                                                                                                                                                   <  2:781449/186‑1 (MQ=255)
                                                                                           atgaGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCtat                                                                                                                                                                                   >  1:781449/1‑186 (MQ=255)
                                                                                             gaGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCTCATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTACTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTa                                                                                                                                             <  1:282694/222‑1 (MQ=255)
                                                                                             gaGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACATCTAATTGATGTGGTTTTTTACTACTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTa                                                                                                                                             >  2:282694/1‑222 (MQ=255)
                                                                                               gCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCAttt                                                                                                               <  1:244486/250‑1 (MQ=255)
                                                                                                ngATTNCTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTa                                                                                                              <  2:558270/249‑1 (MQ=255)
                                                                                                cGATGATTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTGTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTa                                                                                                              <  2:840354/250‑1 (MQ=255)
                                                                                                 caTGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTAGCATAATATCAACACCATTAGCATTTag                                                                                                             <  2:909885/249‑2 (MQ=255)
                                                                                                  aTGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCtttt                                                                                                                                                                           >  1:88899/1‑187 (MQ=255)
                                                                                                  aTGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCtttt                                                                                                                                                                           <  2:88899/187‑1 (MQ=255)
                                                                                                   tGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAGAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATg                                                                                                           >  2:170161/1‑250 (MQ=255)
                                                                                                       ttCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCt                                                                                                       >  1:35849/1‑250 (MQ=255)
                                                                                                          aGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAGAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTc                                                                                                    <  1:170161/250‑1 (MQ=255)
                                                                                                                   tGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAAt                                                                                                                                                                                                                              <  1:545885/119‑1 (MQ=255)
                                                                                                                   tGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAAt                                                                                                                                                                                                                              >  2:545885/1‑119 (MQ=255)
                                                                                                                       aTACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCGTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCtt                                                                                       <  1:256504/250‑1 (MQ=255)
                                                                                                                         aCTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTga                                                                                     <  1:762853/250‑1 (MQ=255)
                                                                                                                                  ttACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAg                                                                                                                                                          <  1:934084/172‑1 (MQ=255)
                                                                                                                                  ttACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAg                                                                                                                                                          >  2:934084/1‑172 (MQ=255)
                                                                                                                                     cTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGTAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACGCCATTAGCATTTAATGCCCTTTCACCCCAGATCCGTGACGACGCCAGGAt                                                                         <  2:20091/250‑1 (MQ=255)
                                                                                                                                       catcaAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACt                                                                                                                                                                                            >  2:668292/1‑133 (MQ=255)
                                                                                                                                       catcaAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACt                                                                                                                                                                                            <  1:668292/133‑1 (MQ=255)
                                                                                                                                        atcaAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAAt                                                                      >  1:826871/1‑250 (MQ=255)
                                                                                                                                          caAAGGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAgg                                                                           >  2:932547/1‑243 (MQ=255)
                                                                                                                                          caAAGGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAgg                                                                           <  1:932547/243‑1 (MQ=255)
                                                                                                                                              aGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGAtg                                                                >  1:175723/1‑250 (MQ=255)
                                                                                                                                               gTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTaa                                                                                                             <  1:676688/204‑1 (MQ=255)
                                                                                                                                               gTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTaa                                                                                                             >  2:676688/1‑204 (MQ=255)
                                                                                                                                                  gcgcCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGAtgttgt                                                            <  2:175723/250‑1 (MQ=255)
                                                                                                                                                    gcCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACGTAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGa                                                                                            <  2:147955/216‑1 (MQ=255)
                                                                                                                                                    gcCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACGTAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGa                                                                                            >  1:147955/1‑216 (MQ=255)
                                                                                                                                                           tCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCgtg                                                   >  1:385098/1‑250 (MQ=255)
                                                                                                                                                                gCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTa                                                                                                                                        <  2:306875/160‑1 (MQ=255)
                                                                                                                                                                gCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTa                                                                                                                                        >  1:306875/1‑160 (MQ=255)
                                                                                                                                                                                        caTCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGAt                                                                 <  2:69980/207‑1 (MQ=255)
                                                                                                                                                                                        caTCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGAt                                                                 >  1:69980/1‑207 (MQ=255)
                                                                                                                                                                                          tCGATTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGt                    <  1:783059/250‑1 (MQ=255)
                                                                                                                                                                                             aTTCATGGGGAGGAATAAAAAAAAACTTACAATCTCTGTAGAAATTCTTTTATACAGCTAATTGATGTGATTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCGCATTAGTTTACATAATATCAACACCATTAGCAttt                                                                                                               <  2:692967/156‑1 (MQ=255)
                                                                                                                                                                                             aTTCATGGGGAGGAATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCAttt                                                                                                               >  1:692967/1‑156 (MQ=255)
                                                                                                                                                                                                     ggaggaATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACa         >  1:673867/1‑250 (MQ=255)
                                                                                                                                                                                                       aggaATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTaa                                                                                                                                                                <  2:188488/97‑1 (MQ=255)
                                                                                                                                                                                                       aggaATAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTaa                                                                                                                                                                >  1:188488/1‑97 (MQ=255)
                                                                                                                                                                                                            tAAAGAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAAttt  >  2:216614/1‑250 (MQ=255)
                                                                                                                                                                                                            tAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCACCTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAAttt  >  1:53499/1‑250 (MQ=255)
                                                                                                                                                                                                            tAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTATCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCa                                                                                                                        <  2:690468/132‑1 (MQ=255)
                                                                                                                                                                                                            tAAAAAAAAACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTATCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCa                                                                                                                        >  1:690468/1‑132 (MQ=255)
                                                                                                                                                                                                             aaaaaaaaACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATg                                  <  2:731826/217‑1 (MQ=255)
                                                                                                                                                                                                             aaaaaaaaACTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATg                                  >  1:731826/1‑217 (MQ=255)
                                                                                                                                                                                                                aaaaaaCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCg                                               >  1:82169/1‑201 (MQ=255)
                                                                                                                                                                                                                aaaaaaCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCg                                               <  2:82169/201‑1 (MQ=255)
                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                
CGCGTGCGCGACCGCCTAAAAACCAACCGCCTACCAGCATCAGGCAACACAGGCCAATGGCAACGATAAGAAAGATAGCGAATGCCCAGTGATGAGCGATGACTTCAGTGGATGTTGACATACTCATTGCTTACTCATCAAAAGTAGCGCCAGATTCACTGCTCTTCACGGCAGATGGACGCCACATCGATTCATGGGGAGGAATAAAAAAAACCTTACAATCACTGTAGAAATTCTTTTATACAGCTAATTGATGTGGTTTTTTACTCCTTTCTATAACCTTTTGTCAACTTTAACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTT  >  NC_007779_cfb/2409616‑2410069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A