Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F999 I1 R1
|
225 |
156.4 |
11709729 |
83.8% |
9812752 |
75.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,279,197 |
A→C |
R145S (AGA→AGC) |
agaA → |
putative N‑acetylgalactosamine‑6‑phosphate deacetylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,279,197 | 0 | A | C | 99.4%
| 435.4
/ NA
| 165 | R145S (AGA→AGC) | agaA | putative N‑acetylgalactosamine‑6‑phosphate deacetylase |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (67/96); total (68/97) |
| Fisher's exact test for biased strand distribution p-value = 4.15e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 168 total aligned reads displayed.
CGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGAGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAG > NC_000913/3279124‑3279269
|
cGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGt > 1:3477568/1‑76 (MQ=255)
gTCTCTGCATCGTGCGCGATTGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTc < 1:5414069/76‑1 (MQ=255)
gTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTc > 2:5429651/1‑76 (MQ=255)
gTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTc < 1:3025789/76‑1 (MQ=255)
gTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTc > 1:3253538/1‑76 (MQ=255)
gTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTc > 1:2671132/1‑76 (MQ=255)
gTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTc > 1:3836685/1‑76 (MQ=255)
gTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTc < 1:89589/76‑1 (MQ=255)
gTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTc > 2:2928461/1‑76 (MQ=255)
gTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTc > 2:3184477/1‑76 (MQ=255)
gTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTc > 2:5100407/1‑76 (MQ=255)
tCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCg < 1:5497898/76‑1 (MQ=255)
cgatGCATCCGGCGCGCATGCTGGGTGTTGATGGTGTTCTGGGAGCGATTAAACCGGGCCAACGCGCCAGcgtcgt > 1:4931495/4‑76 (MQ=255)
gCTGCATCCGGCGCGAATGCTGGGTGATGATGGTGTTCTGGGTGCGCTTAAACGGGGCACACGCGCGAGAGTCCtt > 1:5639484/1‑76 (MQ=255)
tGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTgc > 1:3801678/1‑76 (MQ=255)
cATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTgcgc > 1:1654284/1‑76 (MQ=255)
aTCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCt < 2:4753236/76‑1 (MQ=255)
aTCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCt > 1:3275899/1‑76 (MQ=255)
tCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTCgcgccg > 2:838764/1‑74 (MQ=255)
ccGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTgg > 1:929979/1‑76 (MQ=255)
cGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGa < 1:5139855/76‑1 (MQ=255)
cGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGa < 1:279167/76‑1 (MQ=255)
ggCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGAt < 1:434838/76‑1 (MQ=255)
ggCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGAt > 2:3420016/1‑76 (MQ=255)
gcgcGAATGATGGGTGTTGATGTTGGTCTGTGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATa < 1:3714540/76‑1 (MQ=255)
cgcgAATGCTGGGTGTTGATGGTGTTCTGGGATCTCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAg < 1:3707636/76‑1 (MQ=255)
cgcgAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAg < 1:3593969/76‑1 (MQ=255)
tgtaaTGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACAGGGCAAACTCGCCAGCGTCGTTGCGCTGGATAGc < 1:1674981/73‑1 (MQ=255)
gcgAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGc < 1:3686834/76‑1 (MQ=255)
gcgAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGc < 2:1209479/76‑1 (MQ=255)
gcgAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGGCGTTGCGCTGGATAGc > 2:1871400/1‑76 (MQ=255)
gcgAATGCTGGGCGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGc > 2:1627998/1‑76 (MQ=255)
cgAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGCATAGCg > 2:1427422/1‑76 (MQ=255)
gAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCgg < 2:4693082/76‑1 (MQ=255)
aTGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGc > 2:3885449/1‑76 (MQ=255)
aTGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGc < 2:1680500/76‑1 (MQ=255)
aTGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGc < 1:1078500/76‑1 (MQ=255)
gCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTa > 2:3544001/1‑76 (MQ=255)
gCTGGGTGTTGATGGTGTACTGGTATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTa < 2:4188872/76‑1 (MQ=255)
gCTGGGTGTTGATGGGGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTa > 2:4664713/1‑76 (MQ=255)
cTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTAc < 2:4145309/76‑1 (MQ=255)
cTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTAc < 1:3719832/76‑1 (MQ=255)
gggTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACAt < 2:5590522/76‑1 (MQ=255)
ggTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACAtg > 1:5567163/1‑76 (MQ=255)
gtTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGc > 1:2611957/1‑76 (MQ=255)
tGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTCGCGCTGGATAGCGGGCTACATGTGcaa > 2:3033058/1‑76 (MQ=255)
gATGGTGTGCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGcaac < 2:2603756/76‑1 (MQ=255)
aTGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGcaaca < 1:3166573/76‑1 (MQ=255)
aTGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGcaaca < 1:3002413/76‑1 (MQ=255)
aTGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGcaaca > 1:2226288/1‑76 (MQ=255)
tGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGAt < 2:5302266/55‑1 (MQ=255)
gtgtTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCCACAAAt > 2:3685696/1‑76 (MQ=255)
gtgtTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGGCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAAt > 2:4093684/1‑76 (MQ=255)
tgtTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATc < 1:4181399/76‑1 (MQ=255)
gtTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCt < 1:3838725/76‑1 (MQ=255)
gtTCTGGGATCGCTTAAACCGAGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCGACATGCGCAACAAATCt > 2:1072791/1‑76 (MQ=255)
ggtCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCGACATGGGCAACAAATCt > 2:4888162/3‑76 (MQ=255)
ttCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTg < 2:1474577/76‑1 (MQ=255)
ttCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTg < 2:4651902/76‑1 (MQ=255)
tCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTgg < 1:527255/76‑1 (MQ=255)
cgggggTCGCTGAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGa < 2:63716/74‑1 (MQ=255)
cTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGa > 1:582298/1‑76 (MQ=255)
tGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGAt > 2:2890778/1‑76 (MQ=255)
gggATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGAtt > 2:2876660/1‑76 (MQ=255)
ggATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTc < 1:2442766/76‑1 (MQ=255)
ggATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTc < 2:4476564/76‑1 (MQ=255)
gATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCa < 1:1229457/76‑1 (MQ=255)
gATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCa > 1:5450494/1‑76 (MQ=255)
tCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAgg < 2:4773428/76‑1 (MQ=255)
cGCTTGAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAggg < 1:700035/76‑1 (MQ=255)
cGCTTAAACCGGGCAAACGCGCCGGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAggg < 2:2226288/76‑1 (MQ=255)
gCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGt < 1:2984527/76‑1 (MQ=255)
catAACCCGGGGAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTc < 1:2867463/74‑1 (MQ=255)
cTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGTATTCAGGGTc < 1:1145487/76‑1 (MQ=255)
cTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTc < 2:2487981/76‑1 (MQ=255)
cTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTc < 2:5575086/76‑1 (MQ=255)
ttAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCa < 1:457604/76‑1 (MQ=255)
ttAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCa < 2:4923652/76‑1 (MQ=255)
ttAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCa > 2:3804061/1‑76 (MQ=255)
aCCGGGGCAACTCGCGAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCCGGGTCAATTa < 1:1281257/76‑1 (MQ=255)
aCCGGGCACACGCGCCAGCGTCGTTGCGCTGGCTCGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAAttc < 2:3808900/76‑2 (MQ=255)
aCCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTa > 1:4087760/1‑76 (MQ=255)
aCCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTa < 1:1871400/76‑1 (MQ=255)
gggCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCt > 1:3205976/1‑76 (MQ=255)
gggCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCt > 1:2266445/1‑76 (MQ=255)
ggCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCCGGGTCAATTAGCtt > 2:2047010/1‑76 (MQ=255)
gCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTc > 1:1081060/1‑76 (MQ=255)
gCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTc > 1:5698540/1‑76 (MQ=255)
gCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTc > 2:5229802/1‑76 (MQ=255)
cAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCg > 1:2180747/1‑76 (MQ=255)
cAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCg < 2:4957286/76‑1 (MQ=255)
cAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCg < 1:1235415/76‑1 (MQ=255)
aaaCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGt > 2:215149/1‑76 (MQ=255)
aaaCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGt < 2:3450277/76‑1 (MQ=255)
aaCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGtt > 2:4286987/1‑76 (MQ=255)
aCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGttt < 1:4666356/76‑1 (MQ=255)
cgcgCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGtttt > 2:461603/1‑76 (MQ=255)
gcCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGAt > 1:4534236/1‑76 (MQ=255)
ccAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATa < 2:3274781/76‑1 (MQ=255)
cAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAg < 2:2804083/76‑1 (MQ=255)
|
CGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGAGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAG > NC_000913/3279124‑3279269
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
