Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F999 I1 R1
|
226 |
132.7 |
9745491 |
85.7% |
8351885 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,279,197 |
A→C |
R145S (AGA→AGC) |
agaA → |
putative N‑acetylgalactosamine‑6‑phosphate deacetylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,279,197 | 0 | A | C | 99.3%
| 353.1
/ NA
| 136 | R145S (AGA→AGC) | agaA | putative N‑acetylgalactosamine‑6‑phosphate deacetylase |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (57/77); total (58/78) |
| Fisher's exact test for biased strand distribution p-value = 4.30e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 137 total aligned reads displayed.
GTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGAGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATA > NC_000913/3279125‑3279268
|
tCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCg < 1:4814918/76‑1 (MQ=255)
cGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTAGGATCGCTTAAACCGGGCAAACGCGCCAGcgtcgt < 1:3755498/76‑1 (MQ=255)
gCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGtt > 1:2540/1‑76 (MQ=255)
gCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGtt > 1:3305460/1‑76 (MQ=255)
cTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTg < 1:2604821/76‑1 (MQ=255)
cTGCAGCCGGCGCGAATGCTGTGTGTTGATGGTGTTCTGGGATCGCTTAAACAGGGCAAACGCGCCAGCGTCGTTg < 1:2609335/76‑1 (MQ=255)
tGCATCCGGCGCGAATGCTGGTTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTgc < 1:1800969/76‑1 (MQ=255)
aTCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCt < 1:1813749/76‑1 (MQ=255)
aTCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCt < 2:1891114/76‑1 (MQ=255)
aTCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCt > 2:1521195/1‑76 (MQ=255)
aTCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCt < 1:4156425/76‑1 (MQ=255)
aTCCGGCGCGAATGCTGGGTGTTGATGATGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCt > 2:2175281/1‑76 (MQ=255)
tCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTg < 2:406074/76‑1 (MQ=255)
tCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTg > 1:4060027/1‑76 (MQ=255)
ccGGCGCGAATGCTGGGTGTTGACGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTgg > 1:922380/1‑76 (MQ=255)
cGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGa < 1:4802330/76‑1 (MQ=255)
cGGCGCGAATGCTGGGCGTTGATGGTGTTCTGGGATCGCGTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGa > 1:4471962/1‑76 (MQ=255)
gcgcGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATa < 2:4696279/76‑1 (MQ=255)
gcgAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAgg > 1:298707/1‑75 (MQ=255)
gcgAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGc < 1:618533/76‑1 (MQ=255)
gcgAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGc > 2:3051057/1‑76 (MQ=255)
gcgAAGGCGGGGAGTTGATGGAGTTCGGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTGGAGCTGGATaac > 2:3631847/1‑74 (MQ=255)
gAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCgg < 1:2901759/76‑1 (MQ=255)
gAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCgg < 1:2168611/76‑1 (MQ=255)
gAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCgg < 1:1692866/76‑1 (MQ=255)
aaTGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCggg < 1:88952/76‑1 (MQ=255)
aaTGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCggg < 1:385505/76‑1 (MQ=255)
aTGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGc < 1:4831579/76‑1 (MQ=255)
tGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCt < 1:1240600/76‑1 (MQ=255)
gCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCggg < 2:3641495/73‑1 (MQ=255)
gCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTa > 1:345859/1‑76 (MQ=255)
gCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTa < 2:4529809/76‑1 (MQ=255)
gCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTa > 1:4739/1‑76 (MQ=255)
cTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTAc < 1:2921697/76‑1 (MQ=255)
tGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCcaca > 2:649851/1‑76 (MQ=255)
gggTGTTGATGGTGTTCTGGGATCGCTCAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACAt < 2:1646244/76‑1 (MQ=255)
ggTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACAtg > 1:3003404/1‑76 (MQ=255)
ggTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACAtg < 1:729146/76‑1 (MQ=255)
gtgtTGCTGGCGTTCTGGGATCGCTTAAACCGGGCAAACGCGCAAGAGGCGTGGCGCGGGTTAGCGGGTTGCAtgt > 1:4738228/1‑76 (MQ=255)
gtgtTAATGATGTTCTGGAATCGCTTCGCCCGGTGAAGCGGTCGAGCGTCGTTGCGCTGGATAGCGGGCTACAtgt < 1:2102190/76‑1 (MQ=255)
ggtTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACAtgtg < 2:1913503/75‑1 (MQ=255)
gtTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGc > 2:3984158/1‑76 (MQ=255)
ttGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATGGGGGGCCACTTGTGca > 2:4838946/1‑76 (MQ=255)
ttGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGca < 1:2171133/76‑1 (MQ=255)
gtGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGca < 1:469795/75‑1 (MQ=255)
gATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGcaac > 1:1702613/1‑76 (MQ=255)
gATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGcaac < 2:561842/76‑1 (MQ=255)
gtGGTGTTCTGGGGTCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGcaaca < 2:1476898/75‑1 (MQ=255)
aTGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAg < 1:4615106/58‑1 (MQ=255)
gggTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGcaacaa < 2:4042796/75‑1 (MQ=255)
ggTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACaaa > 1:4732284/1‑76 (MQ=255)
gtgtTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAAt < 1:163841/76‑1 (MQ=255)
gtgtTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAAt < 1:3161566/76‑1 (MQ=255)
gtTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCt < 1:4651526/76‑1 (MQ=255)
gtTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCt > 2:3808621/1‑76 (MQ=255)
ttCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTg < 1:2945014/76‑1 (MQ=255)
tCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATCGCGGGCTACATGTGCAACAAATCTgg > 2:4329370/1‑76 (MQ=255)
tCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTgg > 1:2890488/1‑76 (MQ=255)
tCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTgg > 2:4324823/1‑76 (MQ=255)
cTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGa > 2:4577257/1‑76 (MQ=255)
tGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGAt < 1:3708584/76‑1 (MQ=255)
gggATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGAtt < 1:4682478/76‑1 (MQ=255)
gggATCGCTGAACCCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGAtt < 2:148686/76‑1 (MQ=255)
gATCGCTTAAACCGGGGAGACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCa < 1:2366533/76‑1 (MQ=255)
gATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTcg > 2:1840613/1‑75 (MQ=255)
gATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCa > 1:943695/1‑76 (MQ=255)
gATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCa < 2:881403/76‑1 (MQ=255)
gATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCa < 1:775848/76‑1 (MQ=255)
gATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCa > 2:2879016/1‑76 (MQ=255)
cGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAggg > 1:4323990/1‑76 (MQ=255)
gCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGt < 1:2870376/76‑1 (MQ=255)
gCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGt < 1:4146055/76‑1 (MQ=255)
ttAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCa > 2:3017932/1‑76 (MQ=255)
ttAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCa < 1:670321/76‑1 (MQ=255)
ttAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCa < 2:2146969/76‑1 (MQ=255)
tAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCaa > 2:1994824/1‑76 (MQ=255)
aaaCCGGGCAAACGCGCCAGCTGCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAAt < 2:3539728/76‑1 (MQ=255)
aaaCCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAGTCTGGATTCAGGGTCAAt > 1:1393103/1‑76 (MQ=255)
aaaCCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAAt < 2:3496543/76‑1 (MQ=255)
aaaCCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGCGCAACAAATCTGGATTCAGGGTCAAt > 2:1620250/1‑76 (MQ=255)
cgcGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTa < 2:2762580/74‑1 (MQ=255)
ccGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAg < 1:1069047/76‑1 (MQ=255)
ccGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAg < 1:2749891/76‑1 (MQ=255)
gggCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCt < 1:2629859/76‑1 (MQ=255)
gggAAAGCGCGCGAGGGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCt < 2:5184/76‑1 (MQ=255)
ggCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTTGCtt > 2:904248/1‑76 (MQ=255)
gCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTc < 2:3905167/76‑1 (MQ=255)
cAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCg > 1:1738151/1‑76 (MQ=255)
aaCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGtt > 2:1891261/1‑76 (MQ=255)
aCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGttt > 2:3112095/1‑76 (MQ=255)
aCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGttt < 1:2101681/76‑1 (MQ=255)
gcgcCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTg > 1:3451732/1‑76 (MQ=255)
cgcCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTgg > 2:4451310/1‑75 (MQ=255)
cgcCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGa < 2:3118772/76‑1 (MQ=255)
gcCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGAt < 1:4590115/76‑1 (MQ=255)
gcCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGAt > 1:392712/1‑76 (MQ=255)
gcCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGAt > 1:3885883/1‑76 (MQ=255)
ccAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATa > 1:4318303/1‑76 (MQ=255)
ccAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATa < 2:4666305/76‑1 (MQ=255)
ccAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATa > 2:1104091/1‑76 (MQ=255)
|
GTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGAGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATA > NC_000913/3279125‑3279268
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
