Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F999 I1 R1 226 132.7 9745491 85.7% 8351885 75.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,930,568 +G intergenic (+66/‑373) kup → / → rbsD low affinity potassium transport system/RbsD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,930,5671.G98.2% 304.5 / ‑0.7 114intergenic (+65/‑374)kup/rbsDlow affinity potassium transport system/RbsD
Reads supporting (aligned to +/- strand):  ref base . (2/0);  new base G (46/66);  total (48/66)
Fisher's exact test for biased strand distribution p-value = 1.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 120 total aligned reads displayed.

TATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑T‑GCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATG  >  NC_000913/3930492‑3930637
                                                                              |                                                                      
tatGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGt‑t                                                                         <  1:1512529/76‑1 (MQ=255)
 atGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑T‑g                                                                       <  1:3243817/76‑1 (MQ=255)
  tGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGG‑GGCAAGGTTAGGGT‑T‑gg                                                                      >  2:3316025/1‑75 (MQ=255)
   gTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCCCTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑T‑ggc                                                                     >  2:412867/1‑74 (MQ=255)
        tAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTNGCAA‑GTTATGGT‑TGGCGAGaa                                                                 <  1:2345067/76‑1 (MQ=255)
        tAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGaa                                                                 >  1:2535923/1‑76 (MQ=255)
        tAACGTCCGTCGGGCACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGGGGCAA‑GCTCTGGT‑TGGCGAGaa                                                                 >  1:3183119/1‑76 (MQ=255)
         aaCGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGaaa                                                                <  2:2986870/76‑1 (MQ=255)
           cgtccgtcGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACg                                                              <  1:2847975/76‑1 (MQ=255)
           cgtccgtcGGGTACAGAGTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACg                                                              <  1:3532547/76‑1 (MQ=255)
              ccgtcGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCa                                                           <  2:4695341/76‑1 (MQ=255)
               cgtcGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCaa                                                          >  2:637156/1‑76 (MQ=255)
               cgtcGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCaa                                                          <  1:571915/76‑1 (MQ=255)
               cgtcGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCaa                                                          <  2:2327679/76‑1 (MQ=255)
               cgtcGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCaa                                                          >  2:2809998/1‑76 (MQ=255)
               cgtcGGGTACAGATTGAAAAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCaa                                                          <  1:1512337/76‑1 (MQ=255)
                 gcGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAAc                                                        <  2:812109/75‑1 (MQ=255)
                  cGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACg                                                       <  1:1211521/76‑1 (MQ=255)
                  cGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACg                                                       >  1:1644616/1‑76 (MQ=255)
                   gggTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGt                                                      <  1:2184757/76‑1 (MQ=255)
                   gggTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGt                                                      <  2:2897149/76‑1 (MQ=255)
                    ggTACAGATTGAACAACTGTCGCCGTCTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTa                                                     <  2:2677371/76‑1 (MQ=255)
                    ggTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTa                                                     <  2:3869442/76‑1 (MQ=255)
                     gTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTag                                                    >  1:2821828/1‑76 (MQ=255)
                      tACAGATTGAACGACTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTaga                                                   <  1:4551122/76‑1 (MQ=255)
                      tACAGATTGAACAACTGTCGACCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAGACGCCAAACGCaga                                                   >  1:4402820/1‑76 (MQ=255)
                       aCAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGACACGCCAAACGTagaa                                                  >  2:1851487/1‑76 (MQ=255)
                       aCAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTagaa                                                  >  1:3166723/1‑76 (MQ=255)
                        cAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTagaag                                                 >  2:677402/1‑76 (MQ=255)
                         aGATTGAGCAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTagaaga                                                <  2:1397549/76‑1 (MQ=255)
                          gATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAgaagaa                                               <  2:4552049/76‑1 (MQ=255)
                          gATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAgaagaa                                               >  1:3154303/1‑76 (MQ=255)
                          gATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAgaagaa                                               >  1:2867383/1‑76 (MQ=255)
                           aTTGGACAACTGTGGCAGACGTGCTGGCGCGTGGTGGCAA‑GTGAGGGTGG‑GAGAGAAATGCGAAACGTcgaaggag                                              >  2:3083387/1‑73 (MQ=255)
                           aTTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGaagaag                                              <  2:4167508/76‑1 (MQ=255)
                            ttGAACAACTGTCGCTCTCTTTTTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGCAGAAGt                                             <  1:2147299/76‑1 (MQ=255)
                            ttGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGt                                             >  2:4196503/1‑76 (MQ=255)
                            ttGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGt                                             <  1:2972264/76‑1 (MQ=255)
                            ttGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGt                                             >  2:2517725/1‑76 (MQ=255)
                            ttGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGt                                             >  1:3866881/1‑76 (MQ=255)
                             tGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGtt                                            >  2:2039764/1‑76 (MQ=255)
                                acaccTGTCGCACACATTCTTGCGCGTTGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTc                                         <  1:3785874/76‑1 (MQ=255)
                                acaacTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTc                                         <  1:3746559/76‑1 (MQ=255)
                                acaacTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTc                                         <  2:2014130/76‑1 (MQ=255)
                                  aacTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCaaa                                                         >  1:349184/1‑58 (MQ=255)
                                  aacTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCaaa                                                         <  2:349184/58‑1 (MQ=255)
                                  aacTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAGGAAGTTTTCCa                                       >  1:406113/1‑76 (MQ=255)
                                  aacTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCa                                       <  2:4407751/76‑1 (MQ=255)
                                  aacTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCa                                       <  2:707418/76‑1 (MQ=255)
                                  aacTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCa                                       <  2:1650375/76‑1 (MQ=255)
                                  aacTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCa                                       >  1:1900900/1‑76 (MQ=255)
                                   acTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCAc                                      <  2:4284187/76‑1 (MQ=255)
                                     tGTCGCGCACTTGCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCg                                    <  2:434828/76‑1 (MQ=255)
                                     tGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCg                                    >  2:2838583/1‑76 (MQ=255)
                                      gTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGc                                   <  1:2111033/76‑1 (MQ=255)
                                      gTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGc                                   >  1:1240110/1‑76 (MQ=255)
                                       tCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCt                                  <  1:2039765/76‑1 (MQ=255)
                                       ccGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCt                                  <  2:2120149/75‑1 (MQ=255)
                                        cGCCCAGTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTg                                 <  1:4328651/76‑1 (MQ=255)
                                         gCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGTAG‑GCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGc                                >  1:2809202/1‑76 (MQ=255)
                                         gCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGc                                <  1:4287667/76‑1 (MQ=255)
                                         gCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGc                                <  2:2382629/76‑1 (MQ=255)
                                          cccGCTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCg                               <  2:461689/76‑1 (MQ=255)
                                          cccACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCg                               <  2:4465552/76‑1 (MQ=255)
                                          cccACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCg                               >  1:3284369/1‑76 (MQ=255)
                                           ccAGTTTCGGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCgg                              <  2:1018396/76‑1 (MQ=255)
                                             aCTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTc                            >  2:2017606/1‑76 (MQ=255)
                                              cTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCt                           >  1:3339877/1‑76 (MQ=255)
                                              cTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCt                           >  1:1734165/1‑76 (MQ=255)
                                               tttCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTg                          <  1:1398775/76‑1 (MQ=255)
                                               tttCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTg                          >  2:1379107/1‑76 (MQ=255)
                                                ttCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTgg                         >  2:1530486/1‑76 (MQ=255)
                                                 tCTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGa                        >  1:654067/1‑76 (MQ=255)
                                                  cTGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGaa                       >  1:3580419/1‑76 (MQ=255)
                                                   tGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAg                      <  2:3496414/76‑1 (MQ=255)
                                                   tGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAg                      >  1:1345004/1‑76 (MQ=255)
                                                   tGGCGCGTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCGGCGGTCTGGCAg                      >  2:1658052/1‑76 (MQ=255)
                                                    ggCGCGGGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAgg                     <  2:33605/76‑1 (MQ=255)
                                                     gcgcGTGGTGGCAA‑GTTATGGT‑TGGCGCGAAACTCCACGCGTAGCATAGGTTTTCCGCCGCTGCGGTCTGGAATGa                    <  1:122372/76‑1 (MQ=255)
                                                      cgcgTGGTGGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGAt                   >  1:3237132/1‑76 (MQ=255)
                                                         gtggtgGCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTaa                >  2:4021995/1‑76 (MQ=255)
                                                         gtggtgGCAA‑GTTATGGC‑TGGCGAGACCCGCCACACGTAGAAGAAGTTTTCCACCGCTGCGGTGTGGCAGGATTac                >  2:4030724/1‑75 (MQ=255)
                                                            gtggCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGtt             <  2:670477/76‑1 (MQ=255)
                                                             tggCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTg            <  1:1165428/76‑1 (MQ=255)
                                                              ggCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGc           <  1:103705/76‑1 (MQ=255)
                                                               gCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGcc          <  1:1890864/76‑1 (MQ=255)
                                                               gCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGcc          <  2:590608/76‑1 (MQ=255)
                                                               gCAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGcc          >  1:1374616/1‑76 (MQ=255)
                                                                cAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCg         <  2:2637031/76‑1 (MQ=255)
                                                                cAA‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCg         <  1:817377/76‑1 (MQ=255)
                                                                 aa‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGt              <  1:3843748/70‑1 (MQ=255)
                                                                 aa‑GTTATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGt              >  2:3843748/1‑70 (MQ=255)
                                                                    gTTATGGT‑TGGCGAGCAACGCGAAAGGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGgat      <  2:347713/76‑1 (MQ=255)
                                                                      tATGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGgatga    >  1:1627463/1‑76 (MQ=255)
                                                                       aTGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGgatgat   <  1:4196503/76‑1 (MQ=255)
                                                                       aTGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGgatgat   <  1:504274/76‑1 (MQ=255)
                                                                       aTGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGgatgat   <  1:903371/76‑1 (MQ=255)
                                                                       aTGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTCGAAGGATTAAGTTGCCGgatgat   >  1:2627950/1‑76 (MQ=255)
                                                                        tGGT‑TGGCGAGAAACGCCAAACGTAGATGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTACGTTGCCGgatgatt  <  1:334428/76‑2 (MQ=255)
                                                                        tGGT‑TGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGatgatg  <  1:3583887/76‑1 (MQ=255)
                                                                              |                                                                      
TATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAA‑GTTATGGT‑T‑GCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATG  >  NC_000913/3930492‑3930637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A