Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F999 I1 R1	226	132.7	9745491	85.7%	8351885	75.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,930,677	+G	intergenic (+175/‑264)	kup → / → rbsD	low affinity potassium transport system/RbsD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,930,676	1	.	G	94.6%	233.6 / 8.2	92	intergenic (+174/‑265)	kup/rbsD	low affinity potassium transport system/RbsD
Reads supporting (aligned to +/- strand): ref base . (4/1); new base G (34/53); total (38/54)
Fisher's exact test for biased strand distribution p-value = 1.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑C‑G‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTG  >  NC_000913/3930601‑3930745

ccGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑c                                                                         <  2:4063774/76‑1 (MQ=255)

  gCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑C‑G‑c                                                                     >  2:2326022/1‑76 (MQ=255)

  gCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑C‑G‑c                                                                     >  2:179813/1‑76 (MQ=255)

   cTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCGCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑C‑ggc                                                                     >  2:4259801/1‑74 (MQ=255)

    tGCGGTCTGGAAGGGTTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑C‑ggca                                                                    >  1:525498/1‑73 (MQ=255)

    tGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCCCCTTCTTTATGTCGCATGAGTCGTTGATCCC‑C‑G‑cca                                                                   >  2:310099/1‑74 (MQ=255)

       ggTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAAc                                                                 <  1:637156/76‑1 (MQ=255)

       ggTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAAc                                                                 <  2:2320550/76‑1 (MQ=255)

       ggTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAAc                                                                 <  1:4387101/76‑1 (MQ=255)

         tCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGc                                                               >  1:34606/1‑76 (MQ=255)

          cTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGcc                                                              <  2:4067982/76‑1 (MQ=255)

           tGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGccc                                                             <  2:4514749/76‑1 (MQ=255)

           tGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGccc                                                             >  1:1200473/1‑76 (MQ=255)

           tGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGccc                                                             >  2:3635199/1‑76 (MQ=255)

           tGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGccc                                                             <  1:3604817/76‑1 (MQ=255)

            ggAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCg                                                            <  2:648494/76‑1 (MQ=255)

             gAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGt                                                           <  1:3855528/76‑1 (MQ=255)

               aGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGtag                                                         >  1:1081413/1‑74 (MQ=255)

                ggATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGt                                                        >  1:4829369/1‑76 (MQ=255)

                  aTTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTAt                                                      >  1:3074738/1‑76 (MQ=255)

                  aTTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTAt                                                      <  1:4335102/76‑1 (MQ=255)

                   ttAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTAtt                                                     <  1:997054/76‑1 (MQ=255)

                    tAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTAttt                                                    >  1:1386778/1‑76 (MQ=255)

                     tctttGCCGGCTGCTGGACACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAGCGCCCGTGGTATTTg                                                   <  2:3082002/73‑1 (MQ=255)

                     aaGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTg                                                   <  2:349983/76‑1 (MQ=255)

                      aGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGc                                                  <  2:1900899/76‑1 (MQ=255)

                      aGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGc                                                  <  1:2326791/76‑1 (MQ=255)

                       gTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCg                                                 >  1:3104054/1‑76 (MQ=255)

                        ttGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGt                                                >  1:3667207/1‑76 (MQ=255)

                        ttGCCGGATGATGGAAACCTCCTTCTTTATGCCGCATGAGGCGGTGATCCTCC‑G‑CAAACCCCCGTGGTACTTGCgc                                                >  2:3814321/1‑75 (MQ=255)

                          gCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCt                                              <  2:2749568/76‑1 (MQ=255)

                           ccGGATGATGGAAACCTCCTTCTTTGTGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTg                                             >  1:1257251/1‑76 (MQ=255)

                           ccGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTg                                             >  1:578762/1‑76 (MQ=255)

                            cGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTgc                                            <  1:1749324/76‑1 (MQ=255)

                            cGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTgc                                            >  2:1841972/1‑76 (MQ=255)

                            cGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTgc                                            <  2:3006525/76‑1 (MQ=255)

                            cGGATGATGGAAACCTCCGTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTgc                                            <  2:1393879/76‑1 (MQ=255)

                             ggATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTgcg                                           <  1:3732304/76‑1 (MQ=255)

                              gatgatGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTgcgc                                          <  2:2482302/76‑1 (MQ=255)

                               gtgatgaGAACCTCCTTCTTTATTTGGCGTGAGTCGTTGATTGT‑CGG‑CAAACGCCCGTGGTATTTGGGTCTGcgcg                                         <  2:3437428/75‑1 (MQ=255)

                               atgatgGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGcgcg                                         <  2:4253151/76‑1 (MQ=255)

                               atgatgGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGcgcg                                         <  2:1994769/76‑1 (MQ=255)

                                   tgtaaaCCTCCTTCTGTGTGTCGCAGGAGTCGTGGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCaa                                     <  2:962977/73‑1 (MQ=255)

                                   tgGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCaa                                     <  1:768817/76‑1 (MQ=255)

                                    ggAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAg                                    >  1:3643724/1‑76 (MQ=255)

                                    ggAAACCTCCTTCTTTATGTCGCATGAGGCGTTGATCCT‑CGG‑CAAACGCCCGTGGCATTTGCGTCTGCGCGCCAAg                                    >  2:761220/1‑76 (MQ=255)

                                      aaaCCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCt                                  >  2:2758525/1‑76 (MQ=255)

                                       accctcctTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCtt                                 <  1:603142/74‑2 (MQ=255)

                                        aCCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGt                                >  1:3508980/1‑76 (MQ=255)

                                          ctcctTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTAc                              >  2:905267/1‑76 (MQ=255)

                                          ctcctTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTAc                              <  2:1951180/76‑1 (MQ=255)

                                          ctcctTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTAc                              >  2:3712719/1‑76 (MQ=255)

                                            cctTCTTTATGTCGCATGAGTCTTTGATCCT‑C‑GTCAATCGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACtt                            <  1:131845/76‑1 (MQ=255)

                                            cctTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACtt                            <  1:1250619/76‑1 (MQ=255)

                                             cttcttTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTtg                           <  2:1644615/76‑1 (MQ=255)

                                             cttcttTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTtg                           <  1:378579/76‑1 (MQ=255)

                                              ttcttTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCGGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTtgc                          <  1:3157505/76‑1 (MQ=255)

                                              ttcttTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTtgc                          <  2:2535923/76‑1 (MQ=255)

                                               tcttTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTtgct                         >  1:4803035/1‑76 (MQ=255)

                                               tcttTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTtgct                         >  1:1684428/1‑76 (MQ=255)

                                                cttTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTtgctg                        >  1:2605569/1‑76 (MQ=255)

                                                cttTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTtgctg                        >  2:1731258/1‑76 (MQ=255)

                                                 tttATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTtgctgc                       >  1:4357761/1‑76 (MQ=255)

                                                  ttATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTtgctgct                      <  1:1379108/76‑1 (MQ=255)

                                                   tATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTtgctgctg                     <  1:677402/76‑1 (MQ=255)

                                                   tATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTtgctgctg                     >  2:1769568/1‑76 (MQ=255)

                                                    aTGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTtgctgctgc                    >  2:2660900/1‑76 (MQ=255)

                                                    aTGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTtgctgctgc                    >  2:613666/1‑76 (MQ=255)

                                                    aTGGCGCATGAGTCGTTGATCCT‑CGG‑CAATCGCCCGTGGTATTTGCGTCTGCGCGGCAATCTGTACTtgctgctgc                    <  2:3382428/76‑1 (MQ=255)

                                                      gTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCaa                  <  2:3166723/76‑1 (MQ=255)

                                                      gTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCaa                  <  1:412867/76‑1 (MQ=255)

                                                       tCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAAc                 >  2:202372/1‑76 (MQ=255)

                                                       tCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAAc                 <  1:1658053/76‑1 (MQ=255)

                                                        cGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCCACg                >  2:3920629/1‑76 (MQ=255)

                                                        cGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACg                <  2:1847283/76‑1 (MQ=255)

                                                           aTGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTaa             <  2:2821828/76‑1 (MQ=255)

                                                            tGAGGCGTTGATCCG‑CGG‑CGAAGGCCCGTGGTATTTGCGTCTGCGCGGCAAGGTGTACTTGCTGCTGCAACGTAAt            <  2:4857522/76‑1 (MQ=255)

                                                             gAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATg           <  2:2867383/76‑1 (MQ=255)

                                                             gAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATg           <  1:1067720/76‑1 (MQ=255)

                                                              aGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATgc          >  1:3958494/1‑76 (MQ=255)

                                                               ggcGTTGATCCT‑CGG‑CAAGCGCCCGTGGTGTTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACTTAATgcg         <  2:2851837/74‑1 (MQ=255)

                                                               gTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATgcg         <  2:2811896/76‑1 (MQ=255)

                                                               gTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATgcg         >  1:2225626/1‑76 (MQ=255)

                                                               gTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATgcg         <  1:4420773/76‑1 (MQ=255)

                                                               gTCGTGGATCCA‑CGG‑CACACGCCCGTGGTCTTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATgcg         <  2:3183119/76‑1 (MQ=255)

                                                                 cGTTGATCCT‑CGG‑CAATCGGCCGTGGTATTTGCGACTGCGTGGCAAGCTGTACTTGCTGCTGCGACGTAATGCGCt       <  2:4402820/76‑1 (MQ=255)

                                                                 cGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCt       <  1:606053/76‑1 (MQ=255)

                                                                  gTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTg      >  1:210971/1‑76 (MQ=255)

                                                                  gTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTg      >  1:3888964/1‑76 (MQ=255)

                                                                   ttGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGc     <  1:2517725/76‑1 (MQ=255)

                                                                   ttGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGc     <  2:1734164/76‑1 (MQ=255)

                                                                     gATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCgg   >  2:4652471/1‑75 (MQ=255)

                                                                      aTCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTg  <  1:1731259/76‑1 (MQ=255)

                                                                      aTCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTg  <  2:3580419/76‑1 (MQ=255)

                                                                      aTCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTg  >  1:4196014/1‑76 (MQ=255)

CCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑C‑G‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTG  >  NC_000913/3930601‑3930745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A