Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A0 F0 I1 R1	715	38.4	1606192	96.6%	1551581	120.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,114,502	C→T	465 (TAG→TAA)	wcaM ←	colanic acid biosynthesis protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,114,502	0	C	T	100.0%	145.5 / NA	45	465 (TAG→TAA)	wcaM	colanic acid biosynthesis protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (21/24); total (21/24)

TGTTCGATTTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATCTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAG  >  NC_000913/2114367‑2114630

tGTTCGATTTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTAc                                                                                                                               >  1:746154/1‑139 (MQ=255)

tGTTCGATTTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTCGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTAc                                                                                                                               <  1:801356/139‑1 (MQ=255)

 gTTCGATTTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACt                                                                                                                              >  2:468206/1‑139 (MQ=255)

  ttCGATTTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTc                                                                                                                             >  2:461395/1‑139 (MQ=255)

    cGATTTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTccc                                                                                                                           <  1:766262/139‑1 (MQ=255)

     gATTTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCt                                                                                                                          <  1:218739/139‑1 (MQ=255)

             tCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTAGGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCg                                                                                                                  >  2:478124/1‑139 (MQ=255)

                 cAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCaa                                                                                                              >  1:85109/1‑139 (MQ=255)

                   gCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATc                                                                                                            <  2:770967/139‑1 (MQ=255)

                                       aTTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCcaca                                                                                        >  1:619587/1‑139 (MQ=255)

                                        ttACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCCTTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACAt                                                                                       <  1:468206/139‑1 (MQ=255)

                                        ttACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACAt                                                                                       <  2:275250/139‑1 (MQ=255)

                                            tGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTtctc                                                                                            <  2:38964/130‑1 (MQ=255)

                                            tGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTtctc                                                                                            >  1:38964/1‑130 (MQ=255)

                                                  aCTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTTAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATg                                                                             <  1:565566/139‑1 (MQ=255)

                                                  aCTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATg                                                                             <  2:91022/139‑1 (MQ=255)

                                                       tgATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGAt                                                                        <  2:585184/139‑1 (MQ=255)

                                                       tgATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGAt                                                                        <  1:106473/139‑1 (MQ=255)

                                                        gATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATg                                                                             >  1:343889/1‑133 (MQ=255)

                                                        gATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATg                                                                             <  2:343889/133‑1 (MQ=255)

                                                              aCGCGGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATGAACAATATGGTGATTAATCCt                                                                 >  1:465508/1‑139 (MQ=255)

                                                              aCGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATg                                                                             <  1:320650/127‑1 (MQ=255)

                                                              aCGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATg                                                                             >  2:320650/1‑127 (MQ=255)

                                                                    cTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGat                                                           <  2:619587/139‑1 (MQ=255)

                                                                    cTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGat                                                           >  2:189087/1‑139 (MQ=255)

                                                                     ttCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGata                                                          >  1:652292/1‑139 (MQ=255)

                                                                                 tGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATg                                                                             <  1:660233/108‑1 (MQ=255)

                                                                                 tGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATg                                                                             >  2:660233/1‑108 (MQ=255)

                                                                                     gTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTc                                          <  1:189087/139‑1 (MQ=255)

                                                                                     gTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTc                                          <  1:691124/139‑1 (MQ=255)

                                                                                                   aaGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACAt                                                                                       >  2:353181/1‑80 (MQ=255)

                                                                                                   aaGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACAt                                                                                       <  1:353181/80‑1 (MQ=255)

                                                                                                      tATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCa                         <  2:465508/139‑1 (MQ=255)

                                                                                                      tATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCa                         >  1:496109/1‑139 (MQ=255)

                                                                                                      tATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCa                         <  1:420213/139‑1 (MQ=255)

                                                                                                      tATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACAGAGCTTTGTCCTTTTTCATTCACCGCa                         >  2:294112/1‑139 (MQ=255)

                                                                                                        tGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATg                       >  1:327423/1‑139 (MQ=255)

                                                                                                        tGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTAttcatt                                <  1:774592/130‑1 (MQ=255)

                                                                                                        tGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTAttcatt                                >  2:774592/1‑130 (MQ=255)

                                                                                                           cTCCATTTTTCCAGGAATTGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAATTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGAAc                    <  1:591501/139‑1 (MQ=255)

                                                                                                           cTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGAAc                    >  1:79544/1‑139 (MQ=255)

                                                                                                           cTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGAAc                    >  1:3858/1‑139 (MQ=255)

                                                                                                                             ggTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGAACATTTGCAAGAGAcagcag  <  2:652292/139‑1 (MQ=255)

                                                                                                                              gTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAata                                                                               <  2:25536/61‑1 (MQ=255)

                                                                                                                              gTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAata                                                                               >  1:25536/1‑61 (MQ=255)

TGTTCGATTTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATCTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAG  >  NC_000913/2114367‑2114630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A