Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F1 I1 R1
|
760 |
58.2 |
3022526 |
90.8% |
2744453 |
102.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,114,502 |
C→T |
*465* (TAG→TAA) |
wcaM ← |
colanic acid biosynthesis protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,114,502 | 0 | C | T | 98.3%
| 189.0
/ ‑6.6
| 59 | *465* (TAG→TAA) | wcaM | colanic acid biosynthesis protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (28/30); minor base G (1/0); total (29/30) |
| Fisher's exact test for biased strand distribution p-value = 4.92e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TTCGATTTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATCTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGAAC > NC_000913/2114369‑2114612
|
ttCGATTTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTc > 1:264126/1‑139 (MQ=255)
cGATTTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTccc < 1:1306816/139‑1 (MQ=255)
gATTTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCt < 2:264126/139‑1 (MQ=255)
cGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCgg < 1:1417505/139‑1 (MQ=255)
cccTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAAt < 2:237485/139‑1 (MQ=255)
ccTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAAt < 2:1217743/127‑1 (MQ=255)
ccTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAAt > 1:1217743/1‑127 (MQ=255)
gAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAAtcttc > 1:369350/1‑139 (MQ=255)
aTTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTc > 2:1175327/1‑110 (MQ=255)
aTTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTc < 1:1175327/110‑1 (MQ=255)
aTTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCt < 2:978737/120‑1 (MQ=255)
aTTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCt > 1:978737/1‑120 (MQ=255)
tACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAAtctt > 2:518815/1‑122 (MQ=255)
tACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAAtctt < 1:518815/122‑1 (MQ=255)
cTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATg < 1:1445117/138‑1 (MQ=255)
cTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATg > 2:1445117/1‑138 (MQ=255)
gtgATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCt < 1:781762/90‑1 (MQ=255)
gtgATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCt > 2:781762/1‑90 (MQ=255)
gATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATg > 2:624281/1‑133 (MQ=255)
gATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATg < 1:624281/133‑1 (MQ=255)
cTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTTCTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGat > 2:206118/1‑139 (MQ=255)
cTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTccc < 2:783881/75‑1 (MQ=255)
cTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTccc > 1:783881/1‑75 (MQ=255)
cTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTaa > 1:676935/1‑93 (MQ=255)
cTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTaa < 2:676935/93‑1 (MQ=255)
cTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTtctc < 2:1298244/106‑1 (MQ=255)
cTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTtctc > 1:1298244/1‑106 (MQ=255)
ttGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAAtcttc > 2:1358334/1‑99 (MQ=255)
ttGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAAtcttc < 1:1358334/99‑1 (MQ=255)
ttGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCcac < 1:796166/104‑1 (MQ=255)
ttGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCcac > 2:796166/1‑104 (MQ=255)
tGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCtt > 2:1413780/1‑139 (MQ=255)
tAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAAt < 1:1344351/71‑1 (MQ=255)
tAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAAt > 2:1344351/1‑71 (MQ=255)
ttGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAata < 1:549501/96‑1 (MQ=255)
ttGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAata > 2:549501/1‑96 (MQ=255)
tATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCa > 2:1126106/1‑139 (MQ=255)
tGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCt < 2:1063509/40‑1 (MQ=255)
tGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCt > 1:1063509/1‑40 (MQ=255)
tGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATc > 1:1087110/1‑54 (MQ=255)
tGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATc < 2:1087110/54‑1 (MQ=255)
tGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATg > 2:1474817/1‑85 (MQ=255)
tGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATg < 1:1474817/85‑1 (MQ=255)
gACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCt > 2:906501/1‑121 (MQ=255)
gACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCt < 1:906501/121‑1 (MQ=255)
cTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGAAc < 2:369350/139‑1 (MQ=255)
cTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGAAc < 1:1126106/139‑1 (MQ=255)
taTTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTttctttca > 1:926721/2‑126 (MQ=255)
cATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTttcattca < 2:926721/126‑1 (MQ=255)
ccAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGatat > 2:225194/1‑92 (MQ=255)
ccAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGatat < 1:225194/92‑1 (MQ=255)
gTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTa > 1:1115365/1‑40 (MQ=255)
gTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTa < 2:813907/40‑1 (MQ=255)
gTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTa > 1:813907/1‑40 (MQ=255)
gTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTa < 2:1115365/40‑1 (MQ=255)
gTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTtctc < 2:1030016/48‑1 (MQ=255)
gTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACAt < 2:105520/53‑1 (MQ=255)
gTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACAt > 1:105520/1‑53 (MQ=255)
gTCGCAAATGTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTtctc > 1:1030016/1‑48 (MQ=255)
|
TTCGATTTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATCTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGAAC > NC_000913/2114369‑2114612
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
