Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	774	49.2	2112398	96.9%	2046913	118.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,114,502	C→T	465 (TAG→TAA)	wcaM ←	colanic acid biosynthesis protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,114,502	0	C	T	100.0%	206.2 / NA	63	465 (TAG→TAA)	wcaM	colanic acid biosynthesis protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (33/30); total (33/30)

TGTTCGATTTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATCTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAGT  >  NC_000913/2114367‑2114631

tGTTCGATTTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTAc                                                                                                                                <  2:977550/139‑1 (MQ=255)

tGTTCGATTTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTAc                                                                                                                                <  2:371705/139‑1 (MQ=255)

tGTTCGATTTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTAc                                                                                                                                >  2:591279/1‑139 (MQ=255)

tGTTCGATTTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTAc                                                                                                                                >  1:182787/1‑139 (MQ=255)

  ttCGATTTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTc                                                                                                                              >  1:971481/1‑139 (MQ=255)

  ttCGATTTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTc                                                                                                                              >  1:803873/1‑139 (MQ=255)

  ttCGATTTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTc                                                                                                                              >  2:976274/1‑139 (MQ=255)

  ttCGATTTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTc                                                                                                                              >  2:304828/1‑139 (MQ=255)

  ttCGATTTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTc                                                                                                                              >  1:583140/1‑139 (MQ=255)

   tCGATTTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTcc                                                                                                                             <  2:312395/139‑1 (MQ=255)

     gATTTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCt                                                                                                                           <  2:401669/139‑1 (MQ=255)

                         aTTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATc                                                                                                             <  2:734305/133‑1 (MQ=255)

                         aTTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATc                                                                                                             >  1:734305/1‑133 (MQ=255)

                                 gAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAAtcttc                                                                                               <  1:969407/139‑1 (MQ=255)

                                  aaTTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAAtcttct                                                                                              <  1:655211/139‑1 (MQ=255)

                                  aaTTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAAtcttct                                                                                              >  2:670228/1‑139 (MQ=255)

                                   aTTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTtctc                                                                                             <  1:213306/139‑1 (MQ=255)

                                    ttGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTcc                                                                                            <  1:579045/139‑1 (MQ=255)

                                    ttGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTcc                                                                                            <  1:742195/139‑1 (MQ=255)

                                        ttACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACAt                                                                                        <  2:182787/139‑1 (MQ=255)

                                        ttACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACAt                                                                                        <  1:304828/139‑1 (MQ=255)

                                           cTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTaa                                                                                     <  2:317622/139‑1 (MQ=255)

                                            tGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAAc                                                                                    >  1:506543/1‑139 (MQ=255)

                                               aTTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAat                                                                                 <  1:577934/139‑1 (MQ=255)

                                                  aCTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATg                                                                              <  2:506543/139‑1 (MQ=255)

                                                        ggagTTACGCCTCTTCGTTGTGTATTGCAGTAGCACTGTTCCTAATTATGCGTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGAtt                                                                        >  2:612540/4‑139 (MQ=255)

                                                        gATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTa                                                                                      <  1:485788/125‑1 (MQ=255)

                                                        gATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTa                                                                                      >  2:485788/1‑125 (MQ=255)

                                                              aCGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTtctc                                                                                             >  2:230350/1‑112 (MQ=255)

                                                              aCGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTtctc                                                                                             <  1:230350/112‑1 (MQ=255)

                                                               cgccgcTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTg                                                                 >  2:37990/1‑139 (MQ=255)

                                                                    cTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACAt                                                                                        <  1:840958/111‑1 (MQ=255)

                                                                    cTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACAt                                                                                        >  2:840958/1‑111 (MQ=255)

                                                                                   cAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCt                                                                                                                           >  2:24538/1‑61 (MQ=255)

                                                                                   cAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCt                                                                                                                           <  1:24538/61‑1 (MQ=255)

                                                                                             gTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTttca                                   >  1:482279/1‑139 (MQ=255)

                                                                                             gTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTttca                                   >  2:908759/1‑139 (MQ=255)

                                                                                             gTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTttca                                   >  2:910008/1‑139 (MQ=255)

                                                                                              ttCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCt                                                >  2:695977/1‑125 (MQ=255)

                                                                                              ttCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCt                                                <  1:695977/125‑1 (MQ=255)

                                                                                                 cTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTttcattca                               <  1:976274/139‑1 (MQ=255)

                                                                                                   aaGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCAcc                             >  1:350021/1‑139 (MQ=255)

                                                                                                      tATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACg                                                     <  2:824432/112‑1 (MQ=255)

                                                                                                      tATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACg                                                     >  1:824432/1‑112 (MQ=255)

                                                                                                        tGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAAt                                                                                                   <  2:881409/64‑1 (MQ=255)

                                                                                                        tGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAAt                                                                                                   >  1:881409/1‑64 (MQ=255)

                                                                                                        tGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACa                                                                                   <  2:888750/80‑1 (MQ=255)

                                                                                                        tGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACa                                                                                   >  1:888750/1‑80 (MQ=255)

                                                                                                        tGACTCCATTTTTCCAGGAATGGTCACAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTc                                           >  2:502770/1‑120 (MQ=255)

                                                                                                        tGACTCCATTTTTCCAGGAATGGTCACAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTc                                           <  1:502770/120‑1 (MQ=255)

                                                                                                         gACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGa                       <  2:803873/139‑1 (MQ=255)

                                                                                                          aCTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTa                                                                                                     <  1:818424/60‑1 (MQ=255)

                                                                                                          aCTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTa                                                                                                     >  2:818424/1‑60 (MQ=255)

                                                                                                          aCTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATcc                                                                   <  1:306585/94‑1 (MQ=255)

                                                                                                          aCTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATcc                                                                   >  2:306585/1‑94 (MQ=255)

                                                                                                           cTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGAAc                     <  1:894419/139‑1 (MQ=255)

                                                                                                           cTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGAAc                     >  2:46070/1‑139 (MQ=255)

                                                                                                             ccATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGAACAt                   <  1:46070/139‑1 (MQ=255)

                                                                                                                      cAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGAACATTTGCAagag          >  2:60998/1‑139 (MQ=255)

                                                                                                                        ggAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGAACATTTGCAAGAGAc        >  1:116557/1‑139 (MQ=255)

                                                                                                                          aaTGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGAACATTTGCAAGAGAcag      >  2:174354/1‑139 (MQ=255)

                                                                                                                             ggTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGAACATTTGCAAGAGAcagcag   >  1:15011/1‑139 (MQ=255)

                                                                                                                              gTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAgt  >  1:777451/1‑139 (MQ=255)

TGTTCGATTTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATCTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAGT  >  NC_000913/2114367‑2114631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A