Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I1 R1	771	44.8	2153074	94.1%	2026042	109.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,114,502	C→T	465 (TAG→TAA)	wcaM ←	colanic acid biosynthesis protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,114,502	0	C	T	98.4%	192.7 / ‑3.6	61	coding (1395/1395 nt)	wcaM	colanic acid biosynthesis protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (34/26); minor base . (1/0); total (35/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.12e-01

TGTTCGATTTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATCTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAGTG  >  NC_000913/2114367‑2114632

tGTTCGATTTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTAc                                                                                                                                 >  1:368582/1‑139 (MQ=255)

                              ccTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTAc                                                                                                                                 >  1:756203/1‑109 (MQ=255)

                              ccTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTAc                                                                                                                                 <  2:756203/109‑1 (MQ=255)

                              ccTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACCCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAAtc                                                                                                   >  1:218607/1‑139 (MQ=255)

                                tGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAAt                                                                                                               >  1:225111/1‑125 (MQ=255)

                                tGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAAt                                                                                                               <  2:225111/125‑1 (MQ=255)

                                  aaTTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTTTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATCTTTCCAGGAATGGTCGCAAATTTACTCCCTCATTTCCGGCAATCTAAAGTTAAtcttct                                                                                               >  2:338922/1‑139 (MQ=255)

                                  aaTTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCA‑TTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTtctc                                                                                              >  1:506677/1‑139 (MQ=255)

                                       aTTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCcaca                                                                                          <  2:368582/139‑1 (MQ=255)

                                           cTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTaa                                                                                      <  2:62704/139‑1 (MQ=255)

                                            tGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAAc                                                                                     >  2:966264/1‑139 (MQ=255)

                                              aaTTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTtctc                                                                                              <  1:651603/128‑1 (MQ=255)

                                              aaTTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATCGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTtctc                                                                                              >  2:651603/1‑128 (MQ=255)

                                                  aCTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATg                                                                               <  2:390941/139‑1 (MQ=255)

                                                      gtgATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCcac                                                                                           <  1:317673/123‑1 (MQ=255)

                                                      gtgATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCcac                                                                                           >  2:317673/1‑123 (MQ=255)

                                                      gtgATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGa                                                                           >  1:859184/1‑139 (MQ=255)

                                                        gATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAA‑ATGGTGATTa                                                                        >  2:188747/1‑139 (MQ=255)

                                                              aCGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCt                                                                   >  1:3172/1‑139 (MQ=255)

                                                                    cTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTGCCTCAGTTCCGGCAATCTAAAGTTAAGCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGat                                                             >  1:651901/1‑139 (MQ=255)

                                                                    cTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGat                                                             <  1:966264/139‑1 (MQ=255)

                                                                    cTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGat                                                             <  2:859184/139‑1 (MQ=255)

                                                                    cTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGat                                                             >  1:912850/1‑139 (MQ=255)

                                                                       cGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATc                                                          >  1:69503/1‑139 (MQ=255)

                                                                              gATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTccc                                                                                                                             >  2:224909/1‑65 (MQ=255)

                                                                              gATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTccc                                                                                                                             <  1:224909/65‑1 (MQ=255)

                                                                                 tGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCtt                                                <  2:69503/139‑1 (MQ=255)

                                                                                  gCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCttt                                               >  1:570544/1‑139 (MQ=255)

                                                                                   cAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTg                                              <  2:3172/139‑1 (MQ=255)

                                                                                     gTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTc                                            <  2:570544/139‑1 (MQ=255)

                                                                                     gTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTc                                            <  2:316362/139‑1 (MQ=255)

                                                                                           ttGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTaa                                                                                                     <  1:629029/76‑1 (MQ=255)

                                                                                           ttGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTaa                                                                                                     >  2:629029/1‑76 (MQ=255)

                                                                                           ttGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATg                                                                               <  1:77846/98‑1 (MQ=255)

                                                                                           ttGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATg                                                                               >  2:77846/1‑98 (MQ=255)

                                                                                           ttGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCttttt                                      >  2:509533/1‑139 (MQ=255)

                                                                                           ttGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCttttt                                      >  1:400634/1‑139 (MQ=255)

                                                                                             gTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTttca                                    >  2:308734/1‑139 (MQ=255)

                                                                                             gTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTttca                                    >  2:369217/1‑139 (MQ=255)

                                                                                             gTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTttca                                    >  2:929221/1‑139 (MQ=255)

                                                                                             gTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAAT‑TACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTttcat                                   >  1:604005/1‑139 (MQ=255)

                                                                                                      tATGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCa                           >  1:766655/1‑139 (MQ=255)

                                                                                                        tGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATg                                                                               <  1:385551/85‑1 (MQ=255)

                                                                                                        tGACTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATg                                                                               >  2:385551/1‑85 (MQ=255)

                                                                                                          aCTCCATTTTTCCAGGAATGGTTGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGaa                       >  1:874077/1‑139 (MQ=255)

                                                                                                          aCTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTcc                                                                                             <  1:872837/69‑1 (MQ=255)

                                                                                                          aCTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTcc                                                                                             >  2:872837/1‑69 (MQ=255)

                                                                                                          aCTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGaa                       >  1:83891/1‑139 (MQ=255)

                                                                                                           cTCCATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGAAc                      <  2:784689/139‑1 (MQ=255)

                                                                                                              cATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAAtcttc                                                                                                <  1:697744/62‑1 (MQ=255)

                                                                                                              cATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAAtcttc                                                                                                >  2:697744/1‑62 (MQ=255)

                                                                                                              cATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTtctc                                                                                              <  2:296531/64‑1 (MQ=255)

                                                                                                              cATTTTTCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTtctc                                                                                              >  1:296531/1‑64 (MQ=255)

                                                                                                                  tttCCAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGAACATTTGCa               <  1:369217/139‑1 (MQ=255)

                                                                                                                     ccAGGAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGAACATTTGCAaga            <  1:460777/139‑1 (MQ=255)

                                                                                                                        ggAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATg                                                                               >  1:988731/1‑69 (MQ=255)

                                                                                                                        ggAATGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATg                                                                               <  2:988731/69‑1 (MQ=255)

                                                                                                                            tGGTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACCTTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGAACATTTGCAAGAGAcagca     <  2:218607/139‑1 (MQ=255)

                                                                                                                              gTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAAtcttct                                                                                               >  1:637326/1‑47 (MQ=255)

                                                                                                                              gTCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAAtcttct                                                                                               <  2:637326/47‑1 (MQ=255)

                                                                                                                               tCGCAAATTTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAgtg  >  2:207817/1‑139 (MQ=255)

TGTTCGATTTTTTTCGCCAGCATCAATTACCCTGAATTGATTACTGAATTACTTGTGATGTTACGCCGCTTCGTTGTGGATTGCAGTAGCATTGTTCCTAAGTATGACTCCATTTTTCCAGGAATGGTCGCAAATCTACTCCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAGTG  >  NC_000913/2114367‑2114632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A