Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I2 R1	777	59.2	2955938	92.5%	2734242	106.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	468,510	A→G	E43G (GAG→GGG)	ybaO →	putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	468,510	0	A	G	100.0%	152.6 / NA	43	E43G (GAG→GGG)	ybaO	putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (26/17); total (26/17)

AAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCGCTTTGTCACGGTGGTTA  >  NC_000913/468376‑468644

aaGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGgac                                                                                                                                    <  2:1237368/139‑1 (MQ=255)

  gggCTATGTTAGATAAAATTGACCGTAAGCCGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGgacga                                                                                                                                  <  1:604788/139‑1 (MQ=255)

   ggCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGgacgac                                                                                                                                 >  1:764906/1‑139 (MQ=255)

             gATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTggggga                                                                                                                                     >  1:91296/1‑125 (MQ=255)

             gATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTggggga                                                                                                                                     <  2:91296/125‑1 (MQ=255)

             gATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTa                                                                                                                       >  1:692379/1‑139 (MQ=255)

             gATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTa                                                                                                                       >  2:1118736/1‑139 (MQ=255)

                       aCCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGt                                                                                                             >  2:1405728/1‑139 (MQ=255)

                        ccGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTGTCGGCAAAGTc                                                                                                            >  2:960461/1‑139 (MQ=255)

                        ccGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATATGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTAATCGGCAAAGTc                                                                                                            >  1:1259102/1‑139 (MQ=255)

                         cGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCg                                                                                                           <  1:1118736/139‑1 (MQ=255)

                            aaGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGccc                                                                                                        >  2:1287372/1‑139 (MQ=255)

                                        ttACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCgg                                                                                            <  2:692379/139‑1 (MQ=255)

                                          aCTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGaa                                                                                          >  2:694260/1‑139 (MQ=255)

                                                gcagGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGTCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCAGAAAAAATa                                                                                    >  1:868228/1‑139 (MQ=255)

                                                           cccTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCt                                                                         <  2:764906/139‑1 (MQ=255)

                                                              tctctTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGGTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGACTGAc                                                                      >  2:913115/1‑139 (MQ=255)

                                                              tctctTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAACGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGAc                                                                      >  2:1181519/1‑139 (MQ=255)

                                                                          cACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGAc                                                                      <  2:1314986/127‑1 (MQ=255)

                                                                          cACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGAc                                                                      >  1:1314986/1‑127 (MQ=255)

                                                                            cTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGc                                                                           >  2:744840/1‑120 (MQ=255)

                                                                            cTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGc                                                                           <  1:744840/120‑1 (MQ=255)

                                                                              ggCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATa                                                      >  1:803506/1‑139 (MQ=255)

                                                                               gCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTggggga                                                                                                                                     >  1:643295/1‑59 (MQ=255)

                                                                               gCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTggggga                                                                                                                                     <  2:643295/59‑1 (MQ=255)

                                                                               gCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGacgacg                                                                                                                                >  2:1008836/1‑64 (MQ=255)

                                                                               gCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGacgacg                                                                                                                                <  1:1008836/64‑1 (MQ=255)

                                                                                   aaGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAAc                                                 >  2:1143039/1‑139 (MQ=255)

                                                                                   aaGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAAc                                                 >  2:1410481/1‑139 (MQ=255)

                                                                                   aaGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAAc                                                 >  2:1227903/1‑139 (MQ=255)

                                                                                     gCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGacgacg                                                                                                                                >  2:347658/1‑58 (MQ=255)

                                                                                     gCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGacgacg                                                                                                                                <  1:347658/58‑1 (MQ=255)

                                                                                         ttAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACa                                           >  1:268897/1‑139 (MQ=255)

                                                                                         ttAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACa                                           >  1:1354697/1‑139 (MQ=255)

                                                                                         ttAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACa                                           >  2:966720/1‑139 (MQ=255)

                                                                                          tAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACAt                                          <  2:1354697/139‑1 (MQ=255)

                                                                                            aTCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATca                                        <  1:694260/139‑1 (MQ=255)

                                                                                                 aCAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCAcagca                                   <  1:960461/139‑1 (MQ=255)

                                                                                                      caccCCTTGCTGGAAGCGCCTGAAACGGCTggggga                                                                                                                                     <  2:1070099/36‑1 (MQ=255)

                                                                                                      caccCCTTGCTGGAAGCGCCTGAAACGGCTggggga                                                                                                                                     >  1:1070099/1‑36 (MQ=255)

                                                                                                      caccCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGaa                              >  1:421019/1‑139 (MQ=255)

                                                                                                        ccccTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATca                                        <  2:1389504/127‑1 (MQ=255)

                                                                                                        ccccTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATca                                        >  1:1389504/1‑127 (MQ=255)

                                                                                                         cccTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCg                                                                                                           <  2:632942/59‑1 (MQ=255)

                                                                                                         cccTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCg                                                                                                           >  1:632942/1‑59 (MQ=255)

                                                                                                             tGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTa                                                                                                                             >  2:573495/1‑37 (MQ=255)

                                                                                                             tGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTa                                                                                                                             <  1:573495/37‑1 (MQ=255)

                                                                                                                 ggAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCg                   <  2:268897/139‑1 (MQ=255)

                                                                                                                           gAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCGCTTTGTCACg         <  1:1405728/139‑1 (MQ=255)

                                                                                                                                  cTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCGCTTTGTCACGGTGGTTa  >  1:863719/1‑139 (MQ=255)

AAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCGCTTTGTCACGGTGGTTA  >  NC_000913/468376‑468644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A