Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I1 R1	770	68.5	3470224	91.7%	3182195	103.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	468,510	A→G	E43G (GAG→GGG)	ybaO →	putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	468,510	0	A	G	96.6%	204.9 / ‑0.8	59	coding (128/459 nt)	ybaO	putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (31/26); minor base . (1/1); total (32/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

GGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCGCTTTGTCACGGTGGTTAC  >  NC_000913/468378‑468645

gggCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGgacga                                                                                                                                   <  1:877367/139‑1 (MQ=255)

 ggCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGgacgac                                                                                                                                  >  2:1280961/1‑139 (MQ=255)

           gATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGgacga                                                                                                                                   >  2:1422002/1‑128 (MQ=255)

           gATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGgacga                                                                                                                                   <  1:1422002/128‑1 (MQ=255)

           gATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTa                                                                                                                              <  1:1617368/133‑1 (MQ=255)

           gATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTa                                                                                                                              >  2:1617368/1‑133 (MQ=255)

                aaTTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTggggga                                                                                                                                      >  1:1041015/1‑120 (MQ=255)

                aaTTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTggggga                                                                                                                                      <  2:1041015/120‑1 (MQ=255)

                aaTTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATc                                                                                                                            <  2:1246806/130‑1 (MQ=255)

                aaTTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATc                                                                                                                            >  1:1246806/1‑130 (MQ=255)

                aaTTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGc                                                                                                                   >  2:686147/1‑139 (MQ=255)

                 aTTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCa                                                                                                                  <  1:339341/139‑1 (MQ=255)

                     aCCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGgacga                                                                                                                                   <  2:1191087/118‑1 (MQ=255)

                     aCCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGgacga                                                                                                                                   >  1:1191087/1‑118 (MQ=255)

                       cGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCg                                                                                                            <  1:1458428/139‑1 (MQ=255)

                         tAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGcc                                                                                                          <  1:180061/139‑1 (MQ=255)

                             ctgctgCCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCctgc                                                                                                      <  2:708203/139‑1 (MQ=255)

                                        aCTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGaa                                                                                           >  1:988935/1‑139 (MQ=255)

                                             agcagGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCTGAAAAAAt                                                                                      >  2:1052965/1‑139 (MQ=255)

                                             agcagGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAAt                                                                                      <  1:1280961/139‑1 (MQ=255)

                                             agcagGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAAt                                                                                      >  1:28707/1‑139 (MQ=255)

                                                  gATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGcc                                                                                                          <  2:1148986/114‑1 (MQ=255)

                                                  gATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGcc                                                                                                          >  1:1148986/1‑114 (MQ=255)

                                                       cACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGc                                                                            <  2:1224387/139‑1 (MQ=255)

                                                       cACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGc                                                                            >  1:1108503/1‑139 (MQ=255)

                                                            tctctTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGAc                                                                       <  1:919943/139‑1 (MQ=255)

                                                                    cAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTtg                                                               >  2:435489/1‑139 (MQ=255)

                                                                        cACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGcc                                                                                 >  1:1010278/1‑117 (MQ=255)

                                                                        cACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTCAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGcc                                                                                 <  2:1010278/117‑1 (MQ=255)

                                                                          cTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGgacga                                                                                                                                   >  1:1449062/1‑65 (MQ=255)

                                                                          cTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGgacga                                                                                                                                   <  2:1449062/65‑1 (MQ=255)

                                                                            ggCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTggggga                                                                                                                                      >  1:1007742/1‑60 (MQ=255)

                                                                            ggCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTtgtg                                                             >  2:1725948/1‑133 (MQ=255)

                                                                            ggCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTtgtg                                                             <  1:1725948/133‑1 (MQ=255)

                                                                            ggCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATa                                                       >  1:770541/1‑139 (MQ=255)

                                                                             gCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGG‑GGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATaaa                                                     >  2:294057/1‑139 (MQ=255)

                                                                                 aaGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCg                                                                     >  1:1232653/1‑120 (MQ=255)

                                                                                 aaGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCg                                                                     <  2:1232653/120‑1 (MQ=255)

                                                                                 aaGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAAc                                                  >  2:210106/1‑139 (MQ=255)

                                                                                   gCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGc                                                <  1:601767/139‑1 (MQ=255)

                                                                                       ttAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACa                                            >  1:738977/1‑139 (MQ=255)

                                                                                             tGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCAcag                                      <  1:435489/139‑1 (MQ=255)

                                                                                             tGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCAcag                                      >  2:1431481/1‑139 (MQ=255)

                                                                                               aCAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCgg                                                                             <  2:447188/98‑1 (MQ=255)

                                                                                               aCAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCgg                                                                             >  1:447188/1‑98 (MQ=255)

                                                                                                cAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGacgacg                                                                                                                                 >  1:1534473/1‑45 (MQ=255)

                                                                                                cAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGacgacg                                                                                                                                 <  2:1534473/45‑1 (MQ=255)

                                                                                                  accaccCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCg                                 <  1:210106/139‑1 (MQ=255)

                                                                                                         cTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTa                          >  1:1509890/1‑139 (MQ=255)

                                                                                                                 aaGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCAcag                                      >  2:104928/1‑119 (MQ=255)

                                                                                                                 aaGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCAcag                                      <  1:104928/119‑1 (MQ=255)

                                                                                                                 aaGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCGCt                  >  2:361317/1‑139 (MQ=255)

                                                                                                                  aGCGCCTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGACTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCGCtt                 >  2:833136/1‑139 (MQ=255)

                                                                                                                      ccTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTtgtg                                                             >  2:153792/1‑91 (MQ=255)

                                                                                                                      ccTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTtgtg                                                             <  1:153792/91‑1 (MQ=255)

                                                                                                                      ccTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCg                    <  2:1735090/132‑1 (MQ=255)

                                                                                                                      ccTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCg                    >  1:1735090/1‑132 (MQ=255)

                                                                                                                      ccTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCGCTTTGtt             >  2:1264816/1‑138 (MQ=255)

                                                                                                                       cTGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCGCTTTGTCa            <  2:1509890/139‑1 (MQ=255)

                                                                                                                        tGAAACGGCTGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCGCTTTGTCAc           >  1:343205/1‑139 (MQ=255)

                                                                                                                        tGAAACGGCTGG‑GGACGGCGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCGCTTTGTCACg          <  1:294057/139‑1 (MQ=255)

                                                                                                                                 tGGGGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCGCTTTGTCACGGTGGTTAc  >  1:899498/1‑139 (MQ=255)

GGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCGCTTTGTCACGGTGGTTAC  >  NC_000913/468378‑468645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A