Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F1 I1 R1
|
760 |
58.2 |
3022526 |
90.8% |
2744453 |
102.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
468,510 |
A→G |
E43G (GAG→GGG) |
ybaO → |
putative DNA‑binding transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 468,510 | 0 | A | G | 97.8%
| 153.4
/ ‑4.6
| 45 | E43G (GAG→GGG) | ybaO | putative DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (25/19); total (26/19) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01 |
GGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCGCTTTGTCACG > NC_000913/468379‑468637
|
ggCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTggggga < 2:475467/135‑1 (MQ=255)
ggCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTggggga > 1:475467/1‑135 (MQ=255)
cTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACgg > 1:116479/1‑139 (MQ=255)
aTGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCC‑TGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAt > 2:556887/1‑139 (MQ=255)
aCCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTggggga > 2:519498/1‑115 (MQ=255)
aCCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTggggga < 1:519498/115‑1 (MQ=255)
aCCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGt > 2:1473677/1‑139 (MQ=255)
gCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATc > 2:271756/1‑139 (MQ=255)
tACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGa < 1:1473677/139‑1 (MQ=255)
agcagGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATc > 1:1474793/1‑129 (MQ=255)
agcagGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATc < 2:1474793/129‑1 (MQ=255)
gATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCg < 2:682189/112‑1 (MQ=255)
gATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCg > 1:682189/1‑112 (MQ=255)
ttGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTc > 2:378951/1‑139 (MQ=255)
cACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTa > 2:803942/1‑89 (MQ=255)
cACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTa < 1:803942/89‑1 (MQ=255)
cccTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCt < 2:116479/139‑1 (MQ=255)
ggCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCg < 1:221724/92‑1 (MQ=255)
ggCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCg > 2:221724/1‑92 (MQ=255)
ggCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGcc < 1:937465/94‑1 (MQ=255)
ggCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGcc > 2:937465/1‑94 (MQ=255)
gCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATaa > 2:162086/1‑139 (MQ=255)
aGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACg < 1:162086/139‑1 (MQ=255)
ttAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACgg < 2:1084421/55‑1 (MQ=255)
ttAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACgg > 1:1084421/1‑55 (MQ=255)
ttAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCgg > 2:1247499/1‑67 (MQ=255)
ttAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCgg < 1:1247499/67‑1 (MQ=255)
ttAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCg < 2:1042619/75‑1 (MQ=255)
ttAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCg > 1:1042619/1‑75 (MQ=255)
ccccTTGCTGGAAGCGCCTGAAACGGCTGGGGGacgacg < 1:616729/39‑1 (MQ=255)
ccccTTGCTGGAAGCGCCTGAAACGGCTGGGGGacgacg > 2:616729/1‑39 (MQ=255)
ccccTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATg > 1:726138/1‑139 (MQ=255)
ccccTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATg > 1:384597/1‑139 (MQ=255)
ccccTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATg > 1:451745/1‑139 (MQ=255)
cccTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATgg > 2:1290003/1‑139 (MQ=255)
cTTGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTa > 1:602967/1‑139 (MQ=255)
tGCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTAtt > 1:590395/1‑139 (MQ=255)
gCTGGAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTg > 2:776622/1‑139 (MQ=255)
gAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCtt > 1:590366/1‑37 (MQ=255)
gAAGCGCCTGAAACGGCTGGGGGACGACGGTAT‑CCtt < 2:590366/37‑1 (MQ=255)
aGCGCCTGAAACGGCTGGGGGACGACGGTATCCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCGCt < 1:556887/139‑1 (MQ=255)
ccTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGAc < 1:1467739/81‑1 (MQ=255)
ccTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGAc > 2:1467739/1‑81 (MQ=255)
ccTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCaa < 2:97317/106‑1 (MQ=255)
ccTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCaa > 1:97317/1‑106 (MQ=255)
cTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCt > 2:18747/1‑92 (MQ=255)
cTGAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCt < 1:18747/92‑1 (MQ=255)
gAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCGCTTTGTCACg < 2:451745/139‑1 (MQ=255)
gAAACGGCTGGGGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCGCTTTGTCACg < 1:378951/139‑1 (MQ=255)
|
GGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTAT‑CCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAACATCACAGCAGCGAATGGTATTGCCGCTTTGTCACG > NC_000913/468379‑468637
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
