Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F1 I2 R1 777 59.2 2955938 92.5% 2734242 106.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,106,320 G→A *167* (TAG→TAA)  cpxP → inhibitor of the cpx response, periplasmic adaptor protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,106,3200GA100.0% 147.7 / NA 46*167* (TAG→TAA) cpxPinhibitor of the cpx response, periplasmic adaptor protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/26);  total (20/26)

CCTGTTAACGCCGGAGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGA  >  NC_000913/4106185‑4106454
                                                                                                                                       |                                                                                                                                       
ccTGTTAACGCCGGAGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCATGTTCCCAGtaataa                                                                                                                                      <  2:1269019/139‑1 (MQ=255)
ccTGTTAACGCCGGAGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGtaataa                                                                                                                                      >  1:524242/1‑139 (MQ=255)
   gTTAACGCCGGAGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAAcc                                                                                                                                   >  2:802200/1‑139 (MQ=255)
    ttAACGCCGGAGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAAccc                                                                                                                                  >  2:591610/1‑139 (MQ=255)
           cGGAGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTc                                                                                                                           >  2:4380/1‑139 (MQ=255)
            ggAGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTcc                                                                                                                          <  1:1171677/139‑1 (MQ=255)
            ggAGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTcc                                                                                                                          <  2:1415401/139‑1 (MQ=255)
            ggAGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTcc                                                                                                                          <  1:591610/139‑1 (MQ=255)
            ggAGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTcc                                                                                                                          <  1:242576/139‑1 (MQ=255)
            ggAGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACATTCCCAGTAATAAACCCTGTTTTcc                                                                                                                          <  2:333618/139‑1 (MQ=255)
                    aaGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATa                                                                                                                  <  2:86098/139‑1 (MQ=255)
                     aGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAg                                                                                                                 >  2:1234125/1‑139 (MQ=255)
                             tAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATc                                                                                                         <  1:1072030/139‑1 (MQ=255)
                             tAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATc                                                                                                         >  2:1355158/1‑139 (MQ=255)
                                   agaAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTc                                                                                                   <  2:524242/139‑1 (MQ=255)
                                       aCATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑cc                                                                                              <  1:28941/139‑1 (MQ=255)
                                       aCATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑cc                                                                                              <  1:1355158/139‑1 (MQ=255)
                                                  gAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCtg                                                                                   >  2:1470425/1‑139 (MQ=255)
                                                            gTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGtttt                                                                         >  2:1325326/1‑139 (MQ=255)
                                                               gCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGttttttt                                                                      <  1:802200/139‑1 (MQ=255)
                                                                       tgacgCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAGCTCACGTTCCCAGTAATAAACCCTGTTTTcc                                                                                                                          <  2:1232843/80‑1 (MQ=255)
                                                                       tgacgCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTcc                                                                                                                          >  1:1232843/1‑80 (MQ=255)
                                                                         acgCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAAt                                                            >  1:1120515/1‑139 (MQ=255)
                                                                                       aaGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTa                                              <  1:1259945/139‑1 (MQ=255)
                                                                                         gTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCcaca                                                                                        <  1:603741/95‑1 (MQ=255)
                                                                                         gTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCcaca                                                                                        >  2:603741/1‑95 (MQ=255)
                                                                                            cATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGc                                                                                     >  1:558615/1‑95 (MQ=255)
                                                                                            cATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGc                                                                                     <  2:558615/95‑1 (MQ=255)
                                                                                                gTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGc                                     <  2:1236019/139‑1 (MQ=255)
                                                                                                    aaGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAgat                                 >  1:1115076/1‑139 (MQ=255)
                                                                                                         aTTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTggg                                                                               >  2:156237/1‑88 (MQ=255)
                                                                                                         aTTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTggg                                                                               <  1:156237/88‑1 (MQ=255)
                                                                                                           tGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTgctgct                                                <  1:249111/117‑1 (MQ=255)
                                                                                                           tGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTgctgct                                                >  2:249111/1‑117 (MQ=255)
                                                                                                            gAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCt                         <  1:1325326/139‑1 (MQ=255)
                                                                                                               tagtagCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCctg                                                     <  1:277157/108‑1 (MQ=255)
                                                                                                               tagtagCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCctg                                                     >  2:277157/1‑108 (MQ=255)
                                                                                                                agtagCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCg                     <  2:1190340/139‑1 (MQ=255)
                                                                                                                  tagCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGtt                   >  2:1110878/1‑139 (MQ=255)
                                                                                                                    gCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATcc                                                                                                        >  2:645087/1‑53 (MQ=255)
                                                                                                                    gCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATcc                                                                                                        <  1:645087/53‑1 (MQ=255)
                                                                                                                    gCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAAttt                                                          >  2:1138860/1‑98 (MQ=255)
                                                                                                                    gCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAAttt                                                          <  1:1138860/98‑1 (MQ=255)
                                                                                                                    gCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAACTTGCCTgctgct                                                >  1:107697/1‑108 (MQ=255)
                                                                                                                    gCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAACTTGCCTgctgct                                                <  2:107697/108‑1 (MQ=255)
                                                                                                                         tCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCcaca                                                                                        <  2:151344/63‑1 (MQ=255)
                                                                                                                         tCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCcaca                                                                                        >  1:151344/1‑63 (MQ=255)
                                                                                                                            cGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGt         <  2:1115076/139‑1 (MQ=255)
                                                                                                                                   agtaatAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTg   >  2:278172/1‑139 (MQ=255)
                                                                                                                                   agtaatAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGa  >  2:442886/1‑139 (MQ=255)
                                                                                                                                   agtaatAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGa  <  1:1470425/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                       
CCTGTTAACGCCGGAGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGA  >  NC_000913/4106185‑4106454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A