Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I2 R1 758 59.3 2864096 93.9% 2689386 105.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,106,320 G→A *167* (TAG→TAA)  cpxP → inhibitor of the cpx response, periplasmic adaptor protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,106,3200GA100.0% 198.1 / NA 61*167* (TAG→TAA) cpxPinhibitor of the cpx response, periplasmic adaptor protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/31);  total (30/31)

TTAACGCCGGAGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGG‑TTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACT  >  NC_000913/4106189‑4106442
                                                                                                                                   |                                                                                                                           
ttAACGCCGGAGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAAccc                                                                                                                      >  2:866066/1‑139 (MQ=255)
        ggAGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTcc                                                                                                              <  2:999004/139‑1 (MQ=255)
        ggAGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTcc                                                                                                              <  1:866066/139‑1 (MQ=255)
        ggAGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTcc                                                                                                              <  1:1251156/139‑1 (MQ=255)
          agcagcAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCtt                                                                                                            >  2:1231039/1‑139 (MQ=255)
                    ggTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGaca                                                                                                  <  2:193665/139‑1 (MQ=255)
                    ggTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGaca                                                                                                  <  1:1231039/139‑1 (MQ=255)
                             cGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTg                                                                                         <  2:491518/139‑1 (MQ=255)
                              gagaAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGaca                                                                                                  <  2:109259/129‑1 (MQ=255)
                              gagaAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGaca                                                                                                  >  1:109259/1‑129 (MQ=255)
                                gaAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGa                                                                                                    <  2:219056/125‑1 (MQ=255)
                                gaAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGa                                                                                                    >  1:219056/1‑125 (MQ=255)
                                             cGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCt                                                                         >  1:787747/1‑139 (MQ=255)
                                                           gCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGG‑ttttttt                                                          >  1:475958/1‑139 (MQ=255)
                                                              tgacgtgacgCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTcc                                                                                                              >  2:393447/1‑85 (MQ=255)
                                                              tgacgtgacgCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTcc                                                                                                              <  1:393447/85‑1 (MQ=255)
                                                                   tgacgCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTgg                                                                     <  2:93860/121‑1 (MQ=255)
                                                                   tgacgCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTgg                                                                     >  1:93860/1‑121 (MQ=255)
                                                                     acgCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGG‑TTTTTTTTATCCTCAAt                                                >  2:274993/1‑139 (MQ=255)
                                                                     acgCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAACAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTATCTTCCCCCACATGCTGTGGGGG‑TTTTTTTTATCCTCAAt                                                >  1:957423/1‑139 (MQ=255)
                                                                      cgCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGG‑TTTTTTTTATCCTCAAtt                                               >  1:776564/1‑139 (MQ=255)
                                                                            ggCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGG‑TTTTTTTTCTCCTCAATTTGCctg                                         <  2:957423/139‑1 (MQ=255)
                                                                            ggCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGG‑TTTTTTTTATCCTCAATTTGCctg                                         <  1:274993/139‑1 (MQ=255)
                                                                                 aaaaGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCtg                                                                        <  1:732175/104‑1 (MQ=255)
                                                                                 aaaaGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCtg                                                                        >  2:732175/1‑104 (MQ=255)
                                                                                 aaaaGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGG‑TTTTTTTTATCCTCAATTTGCCTgctgct                                    <  2:787747/139‑1 (MQ=255)
                                                                                   aaGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCa                                                                                                        >  1:434347/1‑70 (MQ=255)
                                                                                   aaGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCa                                                                                                        <  2:434347/70‑1 (MQ=255)
                                                                                       tCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATcc                                                                                            >  1:919551/1‑78 (MQ=255)
                                                                                       tCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATcc                                                                                            <  2:919551/78‑1 (MQ=255)
                                                                                       tCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGG‑TTTTTTTTATCCTCAATTTGCctg                                         >  1:723378/1‑128 (MQ=255)
                                                                                       tCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGG‑TTTTTTTTATCCTCAATTTGCctg                                         <  2:723378/128‑1 (MQ=255)
                                                                                       tCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGG‑TTTTTTTTATCCTCAATTTGCCTgctgct                                    >  2:172502/1‑133 (MQ=255)
                                                                                       tCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGG‑TTTTTTTTATCCTCAATTTGCCTgctgct                                    <  1:172502/133‑1 (MQ=255)
                                                                                       tCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGG‑TTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGc                              >  1:477284/1‑139 (MQ=255)
                                                                                       tCATCGTTGAAGCTATTGAGTAGTAGCAACACACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGG‑TTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGc                              >  1:1292887/1‑139 (MQ=255)
                                                                                                     aTTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGG‑TTTTTTTTATCCTCAATTTGCCTgctgct                                    >  1:490390/1‑119 (MQ=255)
                                                                                                     aTTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGG‑TTTTTTTTATCCTCAATTTGCCTgctgct                                    <  2:490390/119‑1 (MQ=255)
                                                                                                      ttGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGG‑TTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTg               >  2:672845/1‑139 (MQ=255)
                                                                                                       tGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGG‑TTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGc              <  1:948417/139‑1 (MQ=255)
                                                                                                       tGAGTAGTAGCAACACACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGG‑TTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGc              <  2:1292887/139‑1 (MQ=255)
                                                                                                        gAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGG‑TTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCt             <  1:454565/139‑1 (MQ=255)
                                                                                                              tagCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGG‑TTTTTTTTATc                                                      >  1:634772/1‑92 (MQ=255)
                                                                                                              tagCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGG‑TTTTTTTTATc                                                      <  2:634772/92‑1 (MQ=255)
                                                                                                                gCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCcaca                                                                             >  2:616242/1‑68 (MQ=255)
                                                                                                                gCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCcaca                                                                             <  1:616242/68‑1 (MQ=255)
                                                                                                                gCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGG‑TTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTtat     >  2:375862/1‑139 (MQ=255)
                                                                                                                  aaCTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTtata    <  2:475958/139‑1 (MQ=255)
                                                                                                                   aCTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGG‑TTTTTTTTATCCTCAATTTGCctg                                         <  2:1236507/100‑1 (MQ=255)
                                                                                                                   aCTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGG‑TTTTTTTTATCCTCAATTTGCctg                                         >  1:1236507/1‑100 (MQ=255)
                                                                                                                   aCTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGG‑TTTTTTTTATCCTCAATTTGCctg                                         <  2:715668/100‑1 (MQ=255)
                                                                                                                   aCTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGG‑TTTTTTTTATCCTCAATTTGCctg                                         >  1:715668/1‑100 (MQ=255)
                                                                                                                   aCTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGG‑TTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCtt            <  1:1037540/129‑1 (MQ=255)
                                                                                                                   aCTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGG‑TTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCtt            >  2:1037540/1‑129 (MQ=255)
                                                                                                                   aCTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGG‑TTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACt  >  2:544652/1‑139 (MQ=255)
                                                                                                                        cGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGG‑TTTTTTTTATc                                                      >  1:921217/1‑82 (MQ=255)
                                                                                                                        cGTTCCAAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGG‑TTTTTTTAATc                                                      <  2:921217/82‑1 (MQ=255)
                                                                                                                         gTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCca                                                                               >  2:1025023/1‑57 (MQ=255)
                                                                                                                         gTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCca                                                                               <  1:1025023/57‑1 (MQ=255)
                                                                                                                         gTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGG‑TTTTTTTTATCCTCAATTTGCctg                                         >  2:388137/1‑94 (MQ=255)
                                                                                                                         gTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGG‑TTTTTTTTATCCTCAATTTGCctg                                         <  1:388137/94‑1 (MQ=255)
                                                                                                                                   |                                                                                                                           
TTAACGCCGGAGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGG‑TTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACT  >  NC_000913/4106189‑4106442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A