Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I2 R1	766	64.2	3232166	90.9%	2938038	104.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,106,320	G→A	167 (TAG→TAA)	cpxP →	inhibitor of the cpx response, periplasmic adaptor protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,106,320	0	G	A	98.7%	241.0 / NA	74	167 (TAG→TAA)	cpxP	inhibitor of the cpx response, periplasmic adaptor protein
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (37/36); minor base C (0/1); total (37/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTGTTAACGCCGGAGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGT  >  NC_000913/4106185‑4106451

ccTGTTAACGCCGGAGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGtaataa                                                                                                                                   >  1:600313/1‑139 (MQ=255)

 cTGTTAACGCCGGAGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATaaa                                                                                                                                  <  1:514443/139‑1 (MQ=255)

    ttAACGCCGGAGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAAccc                                                                                                                               >  1:543575/1‑139 (MQ=255)

    ttAACGCCGGAGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAAccc                                                                                                                               >  1:503563/1‑139 (MQ=255)

    ttAACGCCGGAGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAAccc                                                                                                                               >  1:1494652/1‑139 (MQ=255)

      aaCGCCGGAGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTg                                                                                                                             <  2:1494652/139‑1 (MQ=255)

             gAGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGGCGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCt                                                                                                                      >  2:164900/1‑139 (MQ=255)

              agcagcAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCtt                                                                                                                     >  2:1277419/1‑139 (MQ=255)

              agcagcAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCtt                                                                                                                     <  1:814514/139‑1 (MQ=255)

              agcagcAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCtt                                                                                                                     >  2:814514/1‑139 (MQ=255)

               gcagcaAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTg                                                                                                                    <  2:543575/139‑1 (MQ=255)

                 agcaAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGcc                                                                                                                  <  2:1145363/139‑1 (MQ=255)

                   caAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCAt                                                                                                                >  1:805259/1‑139 (MQ=255)

                     aGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTcc                                                                                                                       >  1:634412/1‑130 (MQ=255)

                     aGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTcc                                                                                                                       <  2:634412/130‑1 (MQ=255)

                            ttAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCAt                                                                                                       <  2:805259/139‑1 (MQ=255)

                                    gaAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCt                                                                                               >  2:934694/1‑139 (MQ=255)

                                    gaAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCt                                                                                               >  2:311458/1‑139 (MQ=255)

                                          tCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑ccccc                                                                                        <  1:934694/139‑1 (MQ=255)

                                                   aaTGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCAt                                                                                                                <  2:917253/107‑1 (MQ=255)

                                                   aaTGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCAt                                                                                                                >  1:917253/1‑107 (MQ=255)

                                                         gCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGt                                                                         >  1:855894/1‑139 (MQ=255)

                                                             ttGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGaca                                                                                                           <  1:215904/102‑1 (MQ=255)

                                                             ttGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGaca                                                                                                           >  2:215904/1‑102 (MQ=255)

                                                             ttGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTc                                                                                                >  2:439345/1‑113 (MQ=255)

                                                             ttGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTc                                                                                                <  1:439345/113‑1 (MQ=255)

                                                              tGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGtttttt                                                                    >  2:822774/1‑139 (MQ=255)

                                                               gCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGttttttt                                                                   >  2:226811/1‑139 (MQ=255)

                                                                     cgtgacgCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCtt                                                                                              <  2:896449/107‑1 (MQ=255)

                                                                     cgtgacgCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCtt                                                                                              >  1:896449/1‑107 (MQ=255)

                                                                       tgacgCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATa                                                                                                               <  1:557658/88‑1 (MQ=255)

                                                                       tgacgCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATa                                                                                                               >  2:557658/1‑88 (MQ=255)

                                                                       tgacgCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCa                                                           >  2:372852/1‑139 (MQ=255)

                                                                              aTGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGcc                                                    <  2:503563/139‑1 (MQ=255)

                                                                              aTGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGcc                                                    <  2:1036147/139‑1 (MQ=255)

                                                                               tGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCTCGTTCCCAGTAATAAACCCTGTTTTcc                                                                                                                       >  1:1141816/1‑72 (MQ=255)

                                                                               tGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTcc                                                                                                                       <  2:1141816/72‑1 (MQ=255)

                                                                               tGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTcc                                                                                                                       <  1:1341321/72‑1 (MQ=255)

                                                                               tGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTcc                                                                                                                       >  2:1341321/1‑72 (MQ=255)

                                                                               tGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTg                                                                                                  >  2:1295479/1‑93 (MQ=255)

                                                                               tGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTg                                                                                                  <  1:1295479/93‑1 (MQ=255)

                                                                               tGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTg                                                                                                  <  1:436566/93‑1 (MQ=255)

                                                                               tGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTg                                                                                                  >  2:436566/1‑93 (MQ=255)

                                                                                ggCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTACTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCctg                                                  <  2:855894/139‑1 (MQ=255)

                                                                                ggCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCctg                                                  <  1:372852/139‑1 (MQ=255)

                                                                                 gCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTcc                                                                                                                       >  2:565148/1‑70 (MQ=255)

                                                                                 gCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTcc                                                                                                                       <  2:560878/70‑1 (MQ=255)

                                                                                 gCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTcc                                                                                                                       <  1:565148/70‑1 (MQ=255)

                                                                                 gCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTcc                                                                                                                       >  1:560878/1‑70 (MQ=255)

                                                                                        aGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTaa                                          <  1:1277419/139‑1 (MQ=255)

                                                                                          ttCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTc                                                            >  2:920960/1‑119 (MQ=255)

                                                                                          ttCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTc                                                            <  1:920960/119‑1 (MQ=255)

                                                                                            cATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCa                                      <  1:822774/139‑1 (MQ=255)

                                                                                                 ttGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATc                                                               >  2:1385178/1‑109 (MQ=255)

                                                                                                 ttGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATc                                                               <  1:1385178/109‑1 (MQ=255)

                                                                                                         aTTGAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGAttt                         >  1:617936/1‑139 (MQ=255)

                                                                                                            gAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCt                      <  2:1448275/139‑1 (MQ=255)

                                                                                                            gAGTAGTAGCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCt                      <  2:600313/139‑1 (MQ=255)

                                                                                                               tagtagCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATccc                                                                                                    <  2:898533/59‑1 (MQ=255)

                                                                                                               tagtagCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATccc                                                                                                    >  1:898533/1‑59 (MQ=255)

                                                                                                                 gtagCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCg                  <  1:311458/139‑1 (MQ=255)

                                                                                                                    gCAACTCACGTTCCCAGTAATAAACCCTGTTTTcc                                                                                                                       >  2:827852/1‑35 (MQ=255)

                                                                                                                    gCAACTCACGTTCCCAGTAATAAACCCTGTTTTcc                                                                                                                       <  1:827852/35‑1 (MQ=255)

                                                                                                                    gCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCg                  >  2:1177810/1‑135 (MQ=255)

                                                                                                                    gCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCg                  <  1:1177810/135‑1 (MQ=255)

                                                                                                                    gCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTtat              >  1:564133/1‑139 (MQ=255)

                                                                                                                    gCAACTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTtat              >  2:58291/1‑139 (MQ=255)

                                                                                                                       aCTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCctg                                                  >  1:1571630/1‑100 (MQ=255)

                                                                                                                       aCTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCctg                                                  <  2:1571630/100‑1 (MQ=255)

                                                                                                                       aCTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCa                                 <  2:794258/117‑1 (MQ=255)

                                                                                                                       aCTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCa                                 >  1:794258/1‑117 (MQ=255)

                                                                                                                       aCTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACt           <  2:777936/139‑1 (MQ=255)

                                                                                                                        cTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTa                                           >  2:246957/1‑106 (MQ=255)

                                                                                                                        cTCACGTTCCCAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTa                                           <  1:246957/106‑1 (MQ=255)

                                                                                                                                cccAGTAATAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGt  >  2:905784/1‑139 (MQ=255)

CCTGTTAACGCCGGAGCAGCAAGCGGTTTTAAACGAGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTT‑CCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGT  >  NC_000913/4106185‑4106451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A