Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F1 I2 R1 777 59.2 2955938 92.5% 2734242 106.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,218,815 A→G T74A (ACG→GCG)  aceK → isocitrate dehydrogenase kinase/phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,218,8150AG100.0% 223.8 / NA 62T74A (ACG→GCG) aceKisocitrate dehydrogenase kinase/phosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/34);  total (28/34)

CTCCGGTGCGCAGCAGCGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTAC  >  NC_000913/4218679‑4218945
                                                                                                                                        |                                                                                                                                  
cTCCGGTGCGCAGCAGCGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCg                                                                                                                                  >  1:1118719/1‑139 (MQ=255)
    ggTGCGCAGCAGCGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGAcg                                                                                                                              <  2:660776/139‑1 (MQ=255)
       gcgcAGCAGCGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCgg                                                                                                                           <  1:441065/139‑1 (MQ=255)
        cgcAGCAGCGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGc                                                                                                                          >  2:296737/1‑139 (MQ=255)
                 gTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTtact                                                                                                                 <  2:65502/139‑1 (MQ=255)
                 gTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTtact                                                                                                                 <  1:990735/139‑1 (MQ=255)
                  tttCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCgg                                                                                                                                 <  2:1208157/122‑1 (MQ=255)
                  tttCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCgg                                                                                                                                 >  1:1208157/1‑122 (MQ=255)
                  tttCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTtacta                                                                                                                >  1:623969/1‑139 (MQ=255)
                    tCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACg                                                                                                              <  1:296737/139‑1 (MQ=255)
                         cAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTa                                                                                                         <  2:123738/139‑1 (MQ=255)
                                                gcagGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCgg                                                                                                                                 <  2:100300/92‑1 (MQ=255)
                                                gcagGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCgg                                                                                                                                 >  1:100300/1‑92 (MQ=255)
                                                   ggCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCCTTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCg                                                                               >  1:858875/1‑139 (MQ=255)
                                                                  tATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTta                                                                                                                   <  1:1332475/88‑1 (MQ=255)
                                                                  tATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTta                                                                                                                   >  2:1332475/1‑88 (MQ=255)
                                                                    tCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTACCGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCga                                                              >  1:510205/1‑139 (MQ=255)
                                                                           ttACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCg                                                       <  2:21025/139‑1 (MQ=255)
                                                                            tACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCgg                                                      >  1:758600/1‑139 (MQ=255)
                                                                              cGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGgag                                                    >  1:1007443/1‑139 (MQ=255)
                                                                               gATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGgaga                                                   <  2:1007443/139‑1 (MQ=255)
                                                                               gATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGgaga                                                   >  2:1346903/1‑139 (MQ=255)
                                                                                 tcatcaCGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGc                                                 <  2:1404696/139‑1 (MQ=255)
                                                                                 tcatcaCGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGc                                                 <  1:1161280/139‑1 (MQ=255)
                                                                                   atcaCGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCgg                                                                                                                           >  1:515748/1‑63 (MQ=255)
                                                                                   atcaCGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCgg                                                                                                                           <  2:515748/63‑1 (MQ=255)
                                                                                   atcaCGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCtt                                               >  2:848674/1‑139 (MQ=255)
                                                                                        gTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTa                                          <  2:279495/139‑1 (MQ=255)
                                                                                        gTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTa                                          <  2:830062/139‑1 (MQ=255)
                                                                                          tggtctggtcGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAAc                                        <  2:1234377/139‑1 (MQ=255)
                                                                                           ggtctggtcGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCtt                                               <  1:776404/131‑1 (MQ=255)
                                                                                           ggtctggtcGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCtt                                               >  2:776404/1‑131 (MQ=255)
                                                                                           ggtctggtcGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACt                                       <  2:1110223/139‑1 (MQ=255)
                                                                                           ggtctggtcGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACt                                       >  2:253581/1‑139 (MQ=255)
                                                                                           ggtctggtcGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACt                                       >  1:1110223/1‑139 (MQ=255)
                                                                                           ggtctggtcGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACt                                       >  2:458457/1‑139 (MQ=255)
                                                                                           ggtctggtcGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACt                                       <  1:253581/139‑1 (MQ=255)
                                                                                             tctggtcGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTa                                          >  2:1276803/1‑134 (MQ=255)
                                                                                             tctggtcGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTa                                          <  1:1276803/134‑1 (MQ=255)
                                                                                             tctggtcGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTcc                                     >  2:255507/1‑139 (MQ=255)
                                                                                             tctggtcGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTcc                                     <  2:1118719/139‑1 (MQ=255)
                                                                                                 gtcGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCgtgt                                 >  1:678328/1‑139 (MQ=255)
                                                                                                     tGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTg                             <  2:678328/139‑1 (MQ=255)
                                                                                                      ggAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTAcc                                                                        >  1:1471742/1‑95 (MQ=255)
                                                                                                      ggAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTAcc                                                                        <  2:1471742/95‑1 (MQ=255)
                                                                                                            aCTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCgg                                                                                                                           <  2:1428663/38‑1 (MQ=255)
                                                                                                            aCTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCgg                                                                                                                           >  1:1428663/1‑38 (MQ=255)
                                                                                                            aCTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTta                                                                                                                   <  2:258165/46‑1 (MQ=255)
                                                                                                            aCTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTta                                                                                                                   >  1:258165/1‑46 (MQ=255)
                                                                                                                 gcTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGc                                                 <  2:750549/107‑1 (MQ=255)
                                                                                                                 gcTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGc                                                 >  1:750549/1‑107 (MQ=255)
                                                                                                                     cATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGaccac             <  2:623969/139‑1 (MQ=255)
                                                                                                                       ttACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTAGGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACGCCGTGTACTGTCGGt                        <  1:980811/126‑1 (MQ=255)
                                                                                                                       ttACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACcg           <  2:758600/139‑1 (MQ=255)
                                                                                                                       ttACTAACGGCCAAAGTGCAGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGt                        >  2:980811/1‑126 (MQ=255)
                                                                                                                        tACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACcgc          >  2:492184/1‑139 (MQ=255)
                                                                                                                        tACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACcgc          <  2:858875/139‑1 (MQ=255)
                                                                                                                               ggCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCcg                                                                      >  2:1015738/1‑72 (MQ=255)
                                                                                                                               ggCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCcg                                                                      <  1:1015738/72‑1 (MQ=255)
                                                                                                                               ggCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGgaga                                                   >  2:1355970/1‑91 (MQ=255)
                                                                                                                               ggCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGgaga                                                   <  1:1355970/91‑1 (MQ=255)
                                                                                                                                gCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTAc  <  1:255507/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                  
CTCCGGTGCGCAGCAGCGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTAC  >  NC_000913/4218679‑4218945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A