Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F1 I1 R1 771 44.8 2153074 94.1% 2026042 109.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,218,815 A→G T74A (ACG→GCG)  aceK → isocitrate dehydrogenase kinase/phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,218,8150AG100.0% 195.6 / NA 55T74A (ACG→GCG) aceKisocitrate dehydrogenase kinase/phosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/29);  total (26/29)

CGGTGCGCAGCAGCGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTA  >  NC_000913/4218682‑4218944
                                                                                                                                     |                                                                                                                                 
cGGTGCGCAGCAGCGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGAc                                                                                                                              <  2:1069867/139‑1 (MQ=255)
       cagcagCGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCgg                                                                                                                          <  2:283938/136‑1 (MQ=255)
       cagcagCGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCgg                                                                                                                          >  1:283938/1‑136 (MQ=255)
          cagcGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCAtttt                                                                                                                    >  2:520708/1‑139 (MQ=255)
          cagcGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCAtttt                                                                                                                    >  1:289676/1‑139 (MQ=255)
             cGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTtac                                                                                                                 >  1:993821/1‑139 (MQ=255)
              gTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTtact                                                                                                                <  1:334945/139‑1 (MQ=255)
               tttCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTtacta                                                                                                               >  1:352580/1‑139 (MQ=255)
                      cAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTa                                                                                                        <  2:782091/139‑1 (MQ=255)
                        ggCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTaaa                                                                                                      <  2:352580/139‑1 (MQ=255)
                         gCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTtact                                                                                                                >  1:252476/1‑128 (MQ=255)
                         gCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTtact                                                                                                                <  2:252476/128‑1 (MQ=255)
                         gCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAag                                                                                                     >  1:470987/1‑139 (MQ=255)
                             aCTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGTAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCa                                                                                                 <  2:372245/139‑1 (MQ=255)
                                           cagcagGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTtact                                                                                                                <  2:1005518/110‑1 (MQ=255)
                                           cagcagGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTtact                                                                                                                >  1:1005518/1‑110 (MQ=255)
                                                ggCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCgg                                                                                                                          >  1:961692/1‑95 (MQ=255)
                                                ggCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCgg                                                                                                                          <  2:961692/95‑1 (MQ=255)
                                                         aaaCCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCcg                                                                     <  2:368407/139‑1 (MQ=255)
                                                         aaaCCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCcg                                                                     <  2:466997/139‑1 (MQ=255)
                                                         aaaCCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCcg                                                                     <  2:470987/139‑1 (MQ=255)
                                                           aCCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCcgcg                                                                   >  1:19400/1‑139 (MQ=255)
                                                              gTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGAGTATCCGCGCtt                                                                <  2:993821/139‑1 (MQ=255)
                                                                      cTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTtact                                                                                                                >  2:523176/1‑83 (MQ=255)
                                                                      cTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTtact                                                                                                                <  1:523176/83‑1 (MQ=255)
                                                                            gATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGgaga                                                  <  1:783421/139‑1 (MQ=255)
                                                                                atcaCGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGAcgc                                                                                                                            >  1:128706/1‑61 (MQ=255)
                                                                                atcaCGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGAcgc                                                                                                                            <  2:128706/61‑1 (MQ=255)
                                                                                atcaCGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTta                                                                                                                  >  1:988652/1‑71 (MQ=255)
                                                                                atcaCGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTta                                                                                                                  <  2:988652/71‑1 (MQ=255)
                                                                                atcaCGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCtt                                                                <  1:772145/121‑1 (MQ=255)
                                                                                atcaCGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCtt                                                                >  2:772145/1‑121 (MQ=255)
                                                                                        ggtctggtcGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAACGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACt                                      <  2:580202/139‑1 (MQ=255)
                                                                                        ggtctggtcGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGgaga                                                  <  2:449345/127‑1 (MQ=255)
                                                                                        ggtctggtcGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGgaga                                                  >  1:449345/1‑127 (MQ=255)
                                                                                        ggtctggtcGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACt                                      <  2:19400/139‑1 (MQ=255)
                                                                                         gtctggtcGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTc                                     >  1:509007/1‑139 (MQ=255)
                                                                                          tctggtcGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTcc                                    >  2:211478/1‑139 (MQ=255)
                                                                                                cGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTAc                              >  2:368813/1‑139 (MQ=255)
                                                                                                cGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTAc                              <  1:211478/139‑1 (MQ=255)
                                                                                                       cAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGt                       <  1:520708/139‑1 (MQ=255)
                                                                                                        aaCTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACt                                      <  2:510276/123‑1 (MQ=255)
                                                                                                        aaCTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACt                                      >  1:510276/1‑123 (MQ=255)
                                                                                                                    ttACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCgaga                                                           >  2:860372/1‑90 (MQ=255)
                                                                                                                    ttACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCgaga                                                           <  1:860372/90‑1 (MQ=255)
                                                                                                                     tACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACcgc         <  2:509007/139‑1 (MQ=255)
                                                                                                                       cTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTa                                         <  2:76509/105‑1 (MQ=255)
                                                                                                                       cTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTa                                         >  1:76509/1‑105 (MQ=255)
                                                                                                                            ggCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGgaga                                                  <  1:676718/91‑1 (MQ=255)
                                                                                                                            ggCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGgaga                                                  >  2:676718/1‑91 (MQ=255)
                                                                                                                            ggCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACt                                      <  1:665086/103‑1 (MQ=255)
                                                                                                                            ggCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACt                                      >  2:665086/1‑103 (MQ=255)
                                                                                                                            ggCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTa  >  1:119449/1‑139 (MQ=255)
                                                                                                                            ggCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTa  >  1:407834/1‑139 (MQ=255)
                                                                                                                            ggCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTa  >  1:824049/1‑139 (MQ=255)
                                                                                                                                     |                                                                                                                                 
CGGTGCGCAGCAGCGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTA  >  NC_000913/4218682‑4218944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A