Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F1 I1 R1
|
770 |
51.4 |
2439994 |
93.8% |
2288714 |
110.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,218,815 |
A→G |
T74A (ACG→GCG) |
aceK → |
isocitrate dehydrogenase kinase/phosphatase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,218,815 | 0 | A | G | 100.0%
| 169.8
/ NA
| 47 | T74A (ACG→GCG) | aceK | isocitrate dehydrogenase kinase/phosphatase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (25/22); total (25/22) |
CGCAGCAGCGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTA > NC_000913/4218687‑4218944
|
cgcAGCAGCGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATAATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGc > 1:1198514/1‑139 (MQ=255)
gcagcGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCAttt > 2:467015/1‑139 (MQ=255)
cGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAGAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATT‑‑‑‑ACGGCCAAAGTGCGGACGCGGCATTTTTACTACg < 1:629925/139‑1 (MQ=255)
tttCGCACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAATAACCGTATCCGGCTTTACGATCATCCCGTTGGTCTGGTCGTATAGCAACTGCGCTGCATTACTAACGGTCAAAGTTCGGACCGGGCATTTTtaata > 1:1133519/1‑139 (MQ=255)
cAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTa < 2:77896/139‑1 (MQ=255)
gCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAag < 2:1121489/139‑1 (MQ=255)
gCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAag > 1:1121489/1‑139 (MQ=255)
aTGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTacac > 1:250322/1‑139 (MQ=255)
tatatgagacagggCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGAGTTAAAGAGCATTACAcc < 2:803310/127‑1 (MQ=255)
cagcagGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGt > 2:854344/1‑139 (MQ=255)
aCCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGAGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGAATTTTTACTACGTGTTAAAGAGCATTACACGCGGCTGTTGccctgtctcttata > 1:803310/1‑127 (MQ=255)
cATCTTTACGATAATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCgaga < 2:1198514/139‑1 (MQ=255)
tcatcaCGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGGGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGc < 1:303389/139‑1 (MQ=255)
catcaCGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCt > 2:660146/1‑139 (MQ=255)
atcaCGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACg < 1:831549/76‑1 (MQ=255)
atcaCGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACg > 2:831549/1‑76 (MQ=255)
atcaCGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACAcc > 2:267234/1‑95 (MQ=255)
atcaCGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACAcc < 1:267234/95‑1 (MQ=255)
atcaCGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCtt > 1:671771/1‑139 (MQ=255)
atcaCGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCtt > 2:1028274/1‑139 (MQ=255)
atcaCGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTAC‑‑GTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCtttt > 1:484539/1‑139 (MQ=255)
atcaCGTTGGTCTGGTCGAGGATCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGTCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCtt > 1:678215/1‑139 (MQ=255)
tcaCGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAag > 1:606904/1‑83 (MQ=255)
tcaCGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAag < 2:606904/83‑1 (MQ=255)
tcaCGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCttt > 1:1206094/1‑139 (MQ=255)
gTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTa < 1:660146/139‑1 (MQ=255)
gTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTa < 1:854344/139‑1 (MQ=255)
ggtctggtcGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCgg > 2:433696/1‑55 (MQ=255)
ggtctggtcGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCgg < 1:433696/55‑1 (MQ=255)
tctggtcGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACgtg < 2:96136/68‑1 (MQ=255)
tctggtcGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACgtg > 1:96136/1‑68 (MQ=255)
ggtcGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACTCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCgtg < 2:1133519/139‑1 (MQ=255)
ggAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACgtgt > 1:1065224/1‑60 (MQ=255)
ggAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACgtgt < 2:1065224/60‑1 (MQ=255)
ggAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACgtg > 1:504241/1‑59 (MQ=255)
ggAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACgtg < 2:504241/59‑1 (MQ=255)
gAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCa > 2:1089777/1‑45 (MQ=255)
gAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCa < 1:1089777/45‑1 (MQ=255)
gAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTc < 2:346996/139‑1 (MQ=255)
gAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTc < 2:250322/139‑1 (MQ=255)
aaCTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCAGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGtt > 1:126349/1‑139 (MQ=255)
aCTGCGCTGCATTACTAACGGCCAAAGTGCGGAcgc > 2:716743/1‑36 (MQ=255)
cTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGac < 1:1028274/139‑1 (MQ=255)
cATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCcg > 2:428505/1‑82 (MQ=255)
cATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCcg < 1:428505/82‑1 (MQ=255)
aCGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACcgctcgct < 1:1093051/139‑1 (MQ=255)
ggCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTa > 1:528070/1‑139 (MQ=255)
ggCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTa > 2:459365/1‑139 (MQ=255)
|
CGCAGCAGCGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTA > NC_000913/4218687‑4218944
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
