Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F1 I1 R1
|
770 |
51.4 |
2439994 |
93.8% |
2288714 |
110.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
491,776 |
T→C |
V122A (GTT→GCT) |
apt → |
adenine phosphoribosyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 491,776 | 0 | T | C | 100.0%
| 128.9
/ NA
| 36 | V122A (GTT→GCT) | apt | adenine phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (23/13); total (23/13) |
CTGGGCGTTGGCTTTGTACCGGTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAACA > NC_000913/491643‑491905
|
ctgggCGTTGGCTTTGTACCGGTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCtgg < 2:150080/139‑1 (MQ=255)
ggTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCa > 1:384635/1‑139 (MQ=255)
cGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACC‑GGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACcgg < 2:322835/139‑1 (MQ=255)
tAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACcggc > 1:210525/1‑139 (MQ=255)
gCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTAt > 2:1140201/1‑139 (MQ=255)
gcgTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCtggtg < 2:21578/97‑1 (MQ=255)
gcgTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCtggtg > 1:21578/1‑97 (MQ=255)
aaCTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCcgtcgt < 2:210525/139‑1 (MQ=255)
aCTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCa > 1:115859/1‑106 (MQ=255)
aCTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCa < 2:115859/106‑1 (MQ=255)
ttACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTg > 2:498304/1‑139 (MQ=255)
gACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGggtg > 2:1146835/1‑139 (MQ=255)
cACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGAc > 1:646630/1‑139 (MQ=255)
cACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGAc > 1:1018256/1‑139 (MQ=255)
cGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGaa > 2:119866/1‑127 (MQ=255)
cGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGaa < 1:119866/127‑1 (MQ=255)
aGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCAGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGtt < 2:259935/139‑1 (MQ=255)
gagaTCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGtt > 1:580134/1‑134 (MQ=255)
gagaTCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGtt < 2:580134/134‑1 (MQ=255)
agaTCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTAt > 2:210080/1‑139 (MQ=255)
gaTCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGaa < 2:70768/115‑1 (MQ=255)
gaTCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGaa > 1:70768/1‑115 (MQ=255)
cGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTg > 2:1206876/1‑139 (MQ=255)
tGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTc > 2:1069387/1‑139 (MQ=255)
aTGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACcggcgg > 2:849866/1‑58 (MQ=255)
aTGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACcggcgg < 1:849866/58‑1 (MQ=255)
tGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCcgtcgt < 1:570468/91‑1 (MQ=255)
tGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCcgtcgt > 2:570468/1‑91 (MQ=255)
tGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGAt > 2:937650/1‑139 (MQ=255)
tGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGAt > 2:317476/1‑139 (MQ=255)
aTCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTcg > 1:9897/1‑139 (MQ=255)
tCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCt < 2:641197/110‑1 (MQ=255)
tCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCt > 1:641197/1‑110 (MQ=255)
ggCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGc < 2:345202/56‑1 (MQ=255)
ggCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGc > 1:345202/1‑56 (MQ=255)
gCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAACa > 1:487533/1‑139 (MQ=255)
|
CTGGGCGTTGGCTTTGTACCGGTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAACA > NC_000913/491643‑491905
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
