Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	774	49.2	2112398	96.9%	2046913	118.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	491,776	T→C	V122A (GTT→GCT)	apt →	adenine phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	491,776	0	T	C	100.0%	123.7 / NA	35	V122A (GTT→GCT)	apt	adenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (21/14); total (21/14)

GGCGTTGGCTTTGTACCGGTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAAC  >  NC_000913/491646‑491904

ggCGTTGGCTTTGTACCGGTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCtggtgg                                                                                                                          >  1:824625/1‑139 (MQ=255)

           tGTACCGGTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACctg                                                                                                               >  1:989712/1‑139 (MQ=255)

                       tAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACcggc                                                                                                   >  2:608209/1‑139 (MQ=255)

                             gggCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACt                                                                                             >  1:534916/1‑139 (MQ=255)

                                                 ccATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACt                                                                         <  1:1009460/139‑1 (MQ=255)

                                                                            aCGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGc                                              <  2:117308/139‑1 (MQ=255)

                                                                              ggCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTg                                            >  2:1016884/1‑139 (MQ=255)

                                                                              ggCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTg                                            >  1:86874/1‑139 (MQ=255)

                                                                              ggCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTg                                            >  1:734750/1‑139 (MQ=255)

                                                                              ggCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTg                                            >  2:303304/1‑139 (MQ=255)

                                                                              ggCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTg                                            >  1:230843/1‑139 (MQ=255)

                                                                               gCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACcggcgg                                                                                                 >  1:751411/1‑85 (MQ=255)

                                                                               gCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACcggcgg                                                                                                 <  2:751411/85‑1 (MQ=255)

                                                                               gCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTTAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGa                                           >  1:444042/1‑139 (MQ=255)

                                                                               gCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCcgtcgt                                                               <  1:445373/119‑1 (MQ=255)

                                                                               gCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCcgtcgt                                                               >  2:445373/1‑119 (MQ=255)

                                                                                     aTCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACcggc                                                                                                   >  2:241113/1‑77 (MQ=255)

                                                                                     aTCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACcggc                                                                                                   <  1:241113/77‑1 (MQ=255)

                                                                                        aGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGtt                                  <  1:113093/139‑1 (MQ=255)

                                                                                        aGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGtt                                  <  1:303304/139‑1 (MQ=255)

                                                                                          cTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCa                                <  2:721433/139‑1 (MQ=255)

                                                                                            ggAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCAtt                              <  2:1036292/139‑1 (MQ=255)

                                                                                                   cACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAAcc                       <  2:824625/139‑1 (MQ=255)

                                                                                                    aCGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGTACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGc                                              >  1:162908/1‑115 (MQ=255)

                                                                                                    aCGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCg                                                                                >  1:80583/1‑81 (MQ=255)

                                                                                                    aCGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCg                                                                                <  2:80583/81‑1 (MQ=255)

                                                                                                    aCGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGc                                              <  2:162908/115‑1 (MQ=255)

                                                                                                    aCGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCt                      >  2:339158/1‑139 (MQ=255)

                                                                                                    aCGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTAGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCt                      >  1:383527/1‑139 (MQ=255)

                                                                                                           tGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGAt               >  1:22098/1‑139 (MQ=255)

                                                                                                             ccATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGAtct             <  2:734750/139‑1 (MQ=255)

                                                                                                               aTCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTcg           >  2:426917/1‑139 (MQ=255)

                                                                                                                        ggCGACAAAGCTCTGGTGGTGGACGACCTGTTGGCAACcggcgg                                                                                                 <  2:704750/44‑1 (MQ=255)

                                                                                                                        ggCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAAc  >  2:196626/1‑139 (MQ=255)

                                                                                                                        ggCGACAAAGCTCTGGTGGCGGACGACCTGCTGGCAACcggcgg                                                                                                 >  1:704750/1‑44 (MQ=38)

GGCGTTGGCTTTGTACCGGTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAAC  >  NC_000913/491646‑491904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A