Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I1 R1	750	51.0	2595496	93.6%	2429384	106.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	491,776	T→C	V122A (GTT→GCT)	apt →	adenine phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	491,776	0	T	C	100.0%	173.8 / NA	49	V122A (GTT→GCT)	apt	adenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (30/19); total (30/19)

GGTCTGGGCGTTGGCTTTGTACCGGTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAAC  >  NC_000913/491640‑491904

ggtctgggCGTTGGCTTTGTACCGGTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTc                                                                                                                                >  2:633890/1‑139 (MQ=255)

   ctgggCGTTGGCTTTGTACCGGTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCtgg                                                                                                                             <  2:576686/139‑1 (MQ=255)

     gggCGTTGGCTTTGTACCGGTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCtggtg                                                                                                                           <  2:442689/139‑1 (MQ=255)

      ggCGTTGGCTTTGTACCGGTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCtggtgg                                                                                                                          >  1:185464/1‑139 (MQ=255)

        cGTTGGCTTTGTACCGGTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCtggtggtg                                                                                                                        <  2:96602/139‑1 (MQ=255)

              cTTTGTACCGTTCCTTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGgacgac                                                                                                                  <  2:220613/139‑1 (MQ=255)

              cTTTGTACCGGTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGgacgac                                                                                                                  <  2:185464/139‑1 (MQ=255)

                 tGTACCGGTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACctg                                                                                                               >  1:906686/1‑139 (MQ=255)

                             tAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACcggc                                                                                                   >  1:1099824/1‑139 (MQ=255)

                                 ccGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCa                                                                                               <  2:906686/139‑1 (MQ=255)

                                 ccGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCa                                                                                               <  1:633890/139‑1 (MQ=255)

                                                       ccATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACt                                                                         >  1:543565/1‑139 (MQ=255)

                                                                      aCGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTggg                                                          >  1:1149988/1‑139 (MQ=255)

                                                                        gACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGggtg                                                        >  1:1090286/1‑139 (MQ=255)

                                                                             ggAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTc                                                                                                                                >  2:1261949/1‑62 (MQ=255)

                                                                             ggAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTc                                                                                                                                <  1:1261949/62‑1 (MQ=255)

                                                                                    ggCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACcg                                                                                                     >  2:780964/1‑82 (MQ=255)

                                                                                    ggCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACcg                                                                                                     <  1:780964/82‑1 (MQ=255)

                                                                                     gCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGa                                           >  1:1032993/1‑139 (MQ=255)

                                                                                      cACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGc                                                                                     <  2:444185/96‑1 (MQ=255)

                                                                                      cACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGc                                                                                     >  1:444185/1‑96 (MQ=255)

                                                                                      cACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGAc                                          >  2:21033/1‑139 (MQ=255)

                                                                                        ccGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGtt                                                                              >  1:405267/1‑101 (MQ=255)

                                                                                        ccGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGtt                                                                              <  2:405267/101‑1 (MQ=255)

                                                                                        ccGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGc                                        >  1:1117501/1‑139 (MQ=255)

                                                                                         cGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGgacg                                                                                                                    >  1:410522/1‑62 (MQ=255)

                                                                                         cGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGgacg                                                                                                                    <  2:410522/62‑1 (MQ=255)

                                                                                           aTCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCg                                                                                >  1:958323/1‑96 (MQ=255)

                                                                                           aTCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCg                                                                                <  2:958323/96‑1 (MQ=255)

                                                                                           aTCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGAGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGGGGTGAAGGGTCTGACGCTGc                                     >  1:1049603/1‑139 (MQ=255)

                                                                                            tCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGga                                                                                                                      <  1:1084590/57‑1 (MQ=255)

                                                                                            tCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGga                                                                                                                      >  2:1084590/1‑57 (MQ=255)

                                                                                                cTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACcggcgg                                                                                                 <  2:1121267/74‑1 (MQ=255)

                                                                                                cTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACcggcgg                                                                                                 >  1:1121267/1‑74 (MQ=255)

                                                                                                   gagaTCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTa                             >  1:151812/1‑139 (MQ=255)

                                                                                                     gaTCCACGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATc                           >  2:327527/1‑139 (MQ=255)

                                                                                                          aCGTTGGTGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCt                      >  1:178641/1‑139 (MQ=255)

                                                                                                           cGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTg                     >  1:916314/1‑139 (MQ=255)

                                                                                                           cGTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTg                     >  2:60110/1‑139 (MQ=255)

                                                                                                            gTTGATGCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCTTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGt                    <  2:916314/139‑1 (MQ=255)

                                                                                                                  gCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCt                                                             <  1:28856/92‑1 (MQ=255)

                                                                                                                  gCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCt                                                             >  2:28856/1‑92 (MQ=255)

                                                                                                                  gCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGtt                                  >  1:206120/1‑119 (MQ=255)

                                                                                                                  gCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGtt                                  <  2:206120/119‑1 (MQ=255)

                                                                                                                  gCCATCAAACCGGGCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGAtc              >  1:727025/1‑139 (MQ=255)

                                                                                                                              ggCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAAc  >  2:130218/1‑139 (MQ=255)

                                                                                                                              ggCGACAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCACGGCGGCGAAc  >  1:1102751/1‑139 (MQ=255)

                                                                                                                                   cAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGAt                                                                      <  1:1131832/66‑1 (MQ=255)

                                                                                                                                   cAAAGCTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGAt                                                                      >  2:1131832/1‑66 (MQ=255)

GGTCTGGGCGTTGGCTTTGTACCGGTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTTCGATCTCGGCGGCGAAC  >  NC_000913/491640‑491904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A