Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I1 R1	770	51.4	2439994	93.8%	2288714	110.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,245,600	G→A	147 (TAG→TAA)	ycgY →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,245,600	0	G	A	100.0%	136.6 / NA	43	147 (TAG→TAA)	ycgY	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (19/24); total (19/24)

AGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGCGT  >  NC_000913/1245466‑1245717

aGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTaaaaaa                                                                                                                   >  2:942200/1‑139 (MQ=255)

  ccAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGTCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTaaaa                                                                                                                     <  2:444182/135‑1 (MQ=255)

  ccAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTCGAAAAACCGTCTGTGTaaaa                                                                                                                     >  1:444182/1‑135 (MQ=255)

     aCGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCa                                                                                                              <  2:442883/139‑1 (MQ=255)

       gTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCa                                                                                                              <  1:451471/137‑1 (MQ=255)

       gTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCa                                                                                                              >  2:451471/1‑137 (MQ=255)

       gTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATa                                                                                                            >  2:777837/1‑139 (MQ=255)

          ggCGTAAATCGCCGGGTGATGAAAGTGGAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCt                                                                                                         <  2:650508/139‑1 (MQ=255)

           gCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCTGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTa                                                                                                        <  1:777837/139‑1 (MQ=255)

            cGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCa                                                                                                              >  1:258974/1‑132 (MQ=255)

            cGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCa                                                                                                              <  2:258974/132‑1 (MQ=255)

                     ccGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATc                                                                                              >  1:1216821/1‑139 (MQ=255)

                           gatgaAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGa                                                                                        <  2:713773/139‑1 (MQ=255)

                                gaGTGAAACGCTTACCCGTAGAAATGGCGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACg                                                                                   <  1:1145928/138‑1 (MQ=255)

                                            tACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGaa                                                                       >  2:962245/1‑139 (MQ=255)

                                             aCCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGATATCTGCCGACAACGCCATCCGGAGAAg                                                                      >  2:730619/1‑139 (MQ=255)

                                               ccGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGcc                                                                    <  2:1210173/139‑1 (MQ=255)

                                                       aTGACGCTATCTTCGGCAACCACGTATGGCAATCATTCGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACg                                                            >  1:724279/1‑139 (MQ=255)

                                                           cGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGt                                                        <  1:1075115/139‑1 (MQ=255)

                                                            gCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTGTGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTc                                                       <  2:920024/139‑1 (MQ=255)

                                                               aTCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGa                                                    >  2:1214236/1‑139 (MQ=255)

                                                                 cTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGt                                                  <  2:291299/139‑1 (MQ=255)

                                                                    cGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAg                                               >  2:1206926/1‑139 (MQ=255)

                                                                     ggCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAgc                                              <  1:962245/139‑1 (MQ=255)

                                                                     ggCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAgc                                              <  2:920773/139‑1 (MQ=255)

                                                                          ccACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTgg                                         >  2:972126/1‑139 (MQ=255)

                                                                            aCGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGtt                                       <  2:1100761/139‑1 (MQ=255)

                                                                            aCGTATGGCAAACATTCGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGATGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGtt                                       <  2:724279/139‑1 (MQ=255)

                                                                                    cAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAg                                                                                                           >  2:1150533/1‑63 (MQ=255)

                                                                                    cAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAg                                                                                                           <  1:1150533/63‑1 (MQ=255)

                                                                                      aaCATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCAt                                                                                                             <  2:1111182/59‑1 (MQ=255)

                                                                                      aaCATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCAt                                                                                                             >  1:1111182/1‑59 (MQ=255)

                                                                                      aaCATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTggg                             <  1:942200/139‑1 (MQ=255)

                                                                                        cATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGAAAAAAAAACCATAGCTATGGGGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGtt                           >  1:860917/1‑139 (MQ=255)

                                                                                            tGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGc                       <  1:1214236/139‑1 (MQ=255)

                                                                                                 aaTACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCtt                  <  2:1216821/139‑1 (MQ=255)

                                                                                                        cccGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGa           >  1:1098780/1‑139 (MQ=255)

                                                                                                         ccGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAg          >  1:268571/1‑139 (MQ=255)

                                                                                                           gCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGgt                                                                                                    <  2:884037/47‑1 (MQ=255)

                                                                                                           gCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGgt                                                                                                    >  1:884037/1‑47 (MQ=255)

                                                                                                             cAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGc                       <  2:255970/122‑1 (MQ=255)

                                                                                                             cAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGc                       >  1:255970/1‑122 (MQ=255)

                                                                                                             cAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGATAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGtt      >  2:781071/1‑139 (MQ=255)

                                                                                                                gACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACg                                                            <  2:1176925/82‑1 (MQ=255)

                                                                                                                gACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACg                                                            >  1:1176925/1‑82 (MQ=255)

                                                                                                                 aCTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGcgt  <  2:1098780/139‑1 (MQ=255)

AGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGCGT  >  NC_000913/1245466‑1245717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A