Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I2 R1	772	77.0	3769570	91.6%	3452926	105.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,245,600	G→A	147 (TAG→TAA)	ycgY →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,245,600	0	G	A	98.7%	245.6 / ‑5.4	76	147 (TAG→TAA)	ycgY	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (38/37); minor base C (0/1); total (38/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

TAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGCGTCGTTGCTGACTTA  >  NC_000913/1245465‑1245730

taGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACAATTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑Aaaaa                                                                                                                                 <  2:1012412/139‑1 (MQ=255)

   ccAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑aaaa                                                                                                                                  >  1:484690/1‑135 (MQ=255)

   ccAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑aaaa                                                                                                                                  <  2:484690/135‑1 (MQ=255)

      aCGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑aaaaaaaaacc                                                                                                                           >  1:222405/1‑136 (MQ=255)

       cGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCAt                                                                                                                          >  2:918276/1‑139 (MQ=255)

        gTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCa                                                                                                                           >  2:1377495/1‑137 (MQ=255)

        gTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCa                                                                                                                           <  1:1377495/137‑1 (MQ=255)

         tCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAg                                                                                                                        <  1:84588/139‑1 (MQ=255)

           ggCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCt                                                                                                                      <  1:66017/139‑1 (MQ=255)

           ggCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCt                                                                                                                      <  2:1031021/139‑1 (MQ=255)

            gCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTa                                                                                                                     >  1:97028/1‑139 (MQ=255)

                      ccGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATc                                                                                                           >  2:1542215/1‑139 (MQ=255)

                             atgaAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGAc                                                                                                    >  1:22281/1‑139 (MQ=255)

                              tgaAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTTCGCAATACTTCCCGCCAGGACTTGAAAAACAGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACa                                                                                                   <  1:1750398/139‑1 (MQ=255)

                                aaaGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAAc                                                                                                 <  1:918276/139‑1 (MQ=255)

                                 aaGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACg                                                                                                <  2:104396/139‑1 (MQ=255)

                                  aGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACg                                                                                                <  2:222405/139‑1 (MQ=255)

                                   gTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGcc                                                                                              <  2:22281/139‑1 (MQ=255)

                                    tGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGgtgt                                                                                                               <  2:1710578/121‑1 (MQ=255)

                                    tGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGgtgt                                                                                                               >  1:1710578/1‑121 (MQ=255)

                                            ttACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAg                                                                                                                        <  2:1220351/104‑1 (MQ=255)

                                            ttACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAg                                                                                                                        >  1:1220351/1‑104 (MQ=255)

                                              aCCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAg                                                                                   <  2:97028/139‑1 (MQ=255)

                                              aCCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAg                                                                                   <  1:1542215/139‑1 (MQ=255)

                                                   tAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACa                                                                                                   >  2:445035/1‑118 (MQ=255)

                                                   tAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACa                                                                                                   <  1:445035/118‑1 (MQ=255)

                                                   tAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGaa                                                                              >  1:877773/1‑139 (MQ=255)

                                                    aGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAAt                                                                             >  1:540316/1‑139 (MQ=255)

                                                    aGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAAt                                                                             >  1:1825542/1‑139 (MQ=255)

                                                        aTGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGAc                                                                          <  1:43245/138‑1 (MQ=255)

                                                        aTGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGAc                                                                          >  2:43245/1‑138 (MQ=255)

                                                          gACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGtt                                                                       >  2:951312/1‑139 (MQ=255)

                                                           aCGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTg                                                                      >  1:541128/1‑139 (MQ=255)

                                                                aTCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAg                                                                                                                        >  2:1595124/1‑84 (MQ=255)

                                                                aTCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAg                                                                                                                        <  1:1595124/84‑1 (MQ=255)

                                                                      ggCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCt                                                                                                          <  1:1246817/92‑1 (MQ=255)

                                                                      ggCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCt                                                                                                          >  2:1246817/1‑92 (MQ=255)

                                                                      ggCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAgc                                                           >  2:1321234/1‑139 (MQ=255)

                                                                        cAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGgtgt                                                                                                               <  2:1640535/85‑1 (MQ=255)

                                                                        cAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGgtgt                                                                                                               >  1:1640535/1‑85 (MQ=255)

                                                                           ccACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAAc                                                                                                                             >  1:1405055/1‑68 (MQ=255)

                                                                           ccACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAAc                                                                                                                             <  2:1405055/68‑1 (MQ=255)

                                                                           ccACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTgg                                                      >  2:639595/1‑139 (MQ=255)

                                                                             aCGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGtt                                                    <  2:877773/139‑1 (MQ=255)

                                                                              cGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTa                                                   >  1:1053112/1‑139 (MQ=255)

                                                                              cGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTa                                                   >  2:1649602/1‑139 (MQ=255)

                                                                                tATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑aaaaaa                                                                                                                                >  2:1094813/1‑60 (MQ=255)

                                                                                tATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑ACaaaa                                                                                                                                <  1:1094813/60‑1 (MQ=255)

                                                                                      aaaCATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTACGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTgg                                           >  1:873091/1‑139 (MQ=255)

                                                                                       aaCATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTggg                                          >  2:1610528/1‑139 (MQ=255)

                                                                                       aaCATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTggg                                          >  2:1566320/1‑139 (MQ=255)

                                                                                           tttGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTtgt                                      <  2:1538018/139‑1 (MQ=255)

                                                                                           tttGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTtgt                                      <  1:1566320/139‑1 (MQ=255)

                                                                                                 cAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCctct                                <  2:1053112/139‑1 (MQ=255)

                                                                                                    tACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGgtgt                                                                                                               <  1:725434/57‑1 (MQ=255)

                                                                                                    tACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGgtgt                                                                                                               >  2:725434/1‑57 (MQ=255)

                                                                                                         cccGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAgcgc                                                         >  2:1622423/1‑106 (MQ=255)

                                                                                                         cccGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAgcgc                                                         <  1:1622423/106‑1 (MQ=255)

                                                                                                         cccGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGa                        >  1:1018728/1‑139 (MQ=255)

                                                                                                          ccGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCtt                               <  2:1170938/131‑1 (MQ=255)

                                                                                                          ccGCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCtt                               >  1:1170938/1‑131 (MQ=255)

                                                                                                            gCCAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAggg                     >  1:1265602/1‑139 (MQ=255)

                                                                                                              cAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGa                                                                                                     >  1:656799/1‑57 (MQ=255)

                                                                                                              cAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGa                                                                                                     <  2:656799/57‑1 (MQ=255)

                                                                                                              cAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTggg                                          >  2:1147796/1‑116 (MQ=255)

                                                                                                              cAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTggg                                          <  1:1147796/116‑1 (MQ=255)

                                                                                                              cAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCtt                               >  2:1800576/1‑127 (MQ=255)

                                                                                                              cAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCtt                               <  1:1800576/127‑1 (MQ=255)

                                                                                                              cAGGACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGtt                   >  2:484751/1‑139 (MQ=255)

                                                                                                                ggACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACg                                                                                                >  2:1216829/1‑60 (MQ=255)

                                                                                                                ggACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACg                                                                                                <  1:1216829/60‑1 (MQ=255)

                                                                                                                 gACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGcg                <  1:951312/139‑1 (MQ=255)

                                                                                                                 gACTTGAAAAACCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGcg                >  1:1281710/1‑139 (MQ=255)

                                                                                                                         aaaCCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGCGTCGTTGCt        <  2:540316/139‑1 (MQ=255)

                                                                                                                         aaaCCGTCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGCGTCGTTGCt        <  2:873091/139‑1 (MQ=255)

                                                                                                                               tCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGCGTCGTTGCTGACTTa  <  2:541128/139‑1 (MQ=255)

                                                                                                                               tCTGTGT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGCGTCGTTGCTGACTTa  <  2:1281710/139‑1 (MQ=255)

                                                                                                                                 tgtgT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTa                                                              <  2:1014854/77‑1 (MQ=255)

                                                                                                                                 tgtgT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTa                                                              >  1:1014854/1‑77 (MQ=255)

                                                                                                                                 tgtgT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGtt                                                    >  2:613179/1‑87 (MQ=255)

                                                                                                                                 tgtgT‑AAAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGtt                                                    <  1:613179/87‑1 (MQ=255)

TAGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTTCCCGCCAGGACTTGAAAAACCGTCTGTGT‑AGAAAAAACCATAGCTATGGTGTTATCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGCGTCGTTGCTGACTTA  >  NC_000913/1245465‑1245730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A