Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I1 R1	757	76.1	3598070	93.4%	3360597	108.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,245,600	G→A	147 (TAG→TAA)	ycgY →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,245,600	0	G	A	96.9%	198.3 / ‑1.0	64	coding (441/441 nt)	ycgY	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (29/33); minor base . (1/1); total (30/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.56e-01

AGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGCGT  >  NC_000913/1245466‑1245717

aGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTaaaaaa                                                                                                                    >  2:579132/1‑139 (MQ=255)

  ccAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTaaaaaaaa                                                                                                                  >  2:1393342/1‑139 (MQ=255)

  ccAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTaaaaaaaa                                                                                                                  >  1:538362/1‑139 (MQ=255)

  ccAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTaaaaaaaa                                                                                                                  >  1:1761770/1‑139 (MQ=255)

  ccAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTaaaaaaaa                                                                                                                  >  2:732936/1‑139 (MQ=255)

  ccAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCACGACTTGAAAAACCGTCTGTGTaaaaaaaa                                                                                                                  >  1:75359/1‑139 (MQ=255)

   cAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAAc                                                                                                                 <  2:1771060/139‑1 (MQ=255)

   cAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAAc                                                                                                                 <  1:533370/139‑1 (MQ=255)

     aCGTCGGCGTAAATCACCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCa                                                                                                               <  2:285067/139‑1 (MQ=255)

      cGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCAt                                                                                                              >  1:973109/1‑139 (MQ=255)

      cGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTAAGGCAAACATTTGCGCAATACTTCCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCa                                                                                                               <  1:952158/139‑1 (MQ=255)

      cGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTAAGGCAAACATTTGCGCAATACTTCCCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCa                                                                                                               >  2:952158/1‑139 (MQ=255)

       gTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATa                                                                                                             >  2:272557/1‑139 (MQ=255)

          ggCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGcc                                                                                                          <  2:1467383/139‑2 (MQ=255)

          ggCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCt                                                                                                          <  1:410758/139‑1 (MQ=255)

            cGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTaaaa                                                                                                                      >  2:472080/1‑125 (MQ=255)

            cGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCt                                                                                                          >  2:1784084/1‑137 (MQ=255)

            cGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCt                                                                                                          <  1:1784084/137‑1 (MQ=255)

            cGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTAt                                                                                                        >  2:1054916/1‑139 (MQ=255)

                 aTCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGgtgt                                                                                                   >  2:1331080/1‑139 (MQ=255)

                             tgaAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACa                                                                                      <  1:272557/139‑1 (MQ=255)

                                aaGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACg                                                                                   <  1:579132/139‑1 (MQ=255)

                                    gagactcTTACCCGTAGAAATGATGCTATCTTCGGCGATCACGTATGGCAAACATTTGCTAAATATTT‑CCGGCCAGAATTGGAAAAACCGTCTGTGTAAAAAAAACTATATCTATGGTGTTAT‑CTGCCGACACCGCCAt                                                                               <  1:533501/133‑1 (MQ=255)

                                      aaCACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGT‑‑AAAAAACCATAGCTATGgt                                                                                                     <  1:1764890/114‑1 (MQ=255)

                                      aaCACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGT‑‑AAAAAACCATAGCTATGgt                                                                                                     >  2:1764890/1‑114 (MQ=255)

                                           ttACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCt                                                                                                          <  2:574851/106‑1 (MQ=255)

                                           ttACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCt                                                                                                          >  1:574851/1‑106 (MQ=255)

                                           ttACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGgaga                                                                        <  1:1331080/139‑1 (MQ=255)

                                             aCCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAg                                                                      <  1:732936/139‑1 (MQ=255)

                                             aCCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAg                                                                      >  1:1502288/1‑139 (MQ=255)

                                                 gTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACa                                                                                      >  1:802038/1‑119 (MQ=255)

                                                 gTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACa                                                                                      <  2:802038/119‑1 (MQ=255)

                                                   aGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAAt                                                                >  1:817421/1‑139 (MQ=255)

                                                    gAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATg                                                               >  1:912336/1‑139 (MQ=255)

                                                     aaaTGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGa                                                              <  2:973109/139‑1 (MQ=255)

                                                     aaaTGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGa                                                              >  1:1525593/1‑139 (MQ=255)

                                                      aaTGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGAc                                                             <  2:480025/139‑1 (MQ=255)

                                                       aTGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACg                                                            <  2:1502288/139‑1 (MQ=255)

                                                            gCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTc                                                       >  1:1147333/1‑139 (MQ=255)

                                                             cTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCt                                                                                                          <  2:1636420/88‑1 (MQ=255)

                                                             cTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCt                                                                                                          >  1:1636420/1‑88 (MQ=255)

                                                                tCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAAc                                                                                                                 >  2:117781/1‑78 (MQ=255)

                                                                tCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAAc                                                                                                                 <  1:117781/78‑1 (MQ=255)

                                                                 cTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGt                                                  <  2:1333842/139‑1 (MQ=255)

                                                                     ggCAACCACGTATGGCAAACATTTGCGCAATGCTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAgc                                              <  2:1525593/139‑1 (MQ=255)

                                                                     ggCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTTT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGGTGTCGGAGTAAgc                                              <  2:966258/139‑1 (MQ=255)

                                                                     ggCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAgc                                              >  2:229925/1‑139 (MQ=255)

                                                                          ccACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCt                                                                                                          >  2:1643133/1‑75 (MQ=255)

                                                                          ccACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCt                                                                                                          <  1:1643133/75‑1 (MQ=255)

                                                                              gTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTaa                                     <  2:281549/139‑1 (MQ=255)

                                                                                           ttGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTtgtg                        >  1:296953/1‑139 (MQ=255)

                                                                                            tGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCa                                                                                                               >  2:201994/1‑52 (MQ=255)

                                                                                            tGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCa                                                                                                               <  1:201994/52‑1 (MQ=255)

                                                                                            tGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGc                       <  1:229925/139‑1 (MQ=255)

                                                                                            tGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGc                       >  2:1303134/1‑139 (MQ=255)

                                                                                               gcAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAG‑AGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCctct                   <  1:1054916/139‑1 (MQ=255)

                                                                                                 aaTACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCtt                  <  2:790636/139‑1 (MQ=255)

                                                                                                   tACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTg                >  1:526313/1‑139 (MQ=255)

                                                                                                         cccGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAAc                                                                                    <  2:845704/66‑1 (MQ=255)

                                                                                                         cccGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAAc                                                                                    >  1:845704/1‑66 (MQ=255)

                                                                                                         cccGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGa           <  2:538362/139‑1 (MQ=255)

                                                                                                         cccGCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGa           >  2:857182/1‑139 (MQ=255)

                                                                                                         cccGCCAGGACTTGAAAAACCGTCTGAGTAAAAAAAAACATGGCTATGATGTTAT‑CTGCCCAGAACCCAACCCGCAGAACCCGAATGACATCCTCGGAGTACGCGCCGGATAAAGTATGAAATGTGccgatctgtgtgt           >  1:3523/1‑128 (MQ=255)

                                                                                                            gCCAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAggg        >  2:460130/1‑139 (MQ=255)

                                                                                                              cAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGtt      >  1:953100/1‑139 (MQ=255)

                                                                                                              cAGGACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTC‑TTGGTTGAGGGTTg     >  1:1740827/1‑139 (MQ=255)

                                                                                                                ggACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACg                                                            >  1:227241/1‑83 (MQ=255)

                                                                                                                ggACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACg                                                            <  2:227241/83‑1 (MQ=255)

                                                                                                                ggACTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGc    >  2:385754/1‑139 (MQ=255)

                                                                                                                  aCTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTATCCTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGcg   <  2:1761770/139‑1 (MQ=255)

                                                                                                                  aCTTGAAAAACCGTCTGTGTAAAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGcgt  <  2:296953/139‑1 (MQ=255)

AGCCAACGTCGGCGTAAATCGCCGGGTGATGAAAGTGAAACACTTACCCGTAGAAATGACGCTATCTTCGGCAACCACGTATGGCAAACATTTGCGCAATACTT‑CCCGCCAGGACTTGAAAAACCGTCTGTGTAGAAAAAACCATAGCTATGGTGTTAT‑CTGCCGACAACGCCATCCGGAGAAGCCGAATGACGTTGTCGGAGTAAGCGCTGGTTAAGGTGTGGGTTGTGCCTCTTTGGTTGAGGGTTGCGT  >  NC_000913/1245466‑1245717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A