Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I1 R1	770	51.4	2439994	93.8%	2288714	110.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,620,207	G→A	73 (TAG→TAA)	marB →	periplasmic mar operon regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,620,207	0	G	A	100.0%	208.3 / NA	65	73 (TAG→TAA)	marB	periplasmic mar operon regulator
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (34/31); total (34/31)

TACTTCTTGTGCCAATGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAG  >  NC_000913/1620075‑1620340

tACTTCTTGTGCCAATGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGtt                                                                                                                                 <  2:301460/139‑1 (MQ=255)

tACTTCTTGTGCCAATGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGtt                                                                                                                                 <  2:768952/139‑1 (MQ=255)

                   gTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGa                                                                                                                       >  2:241380/1‑130 (MQ=255)

                   gTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGa                                                                                                                       <  1:241380/130‑1 (MQ=255)

                    tggttggtCCGCTATGGCAGAGACACCCTCCGTTTGATATAAATCACATGGGTACTGTCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACTCGCTATGTAATTTGTTCTAGCCCCGACATCTCgggg                                                                                                             <  1:608272/139‑1 (MQ=255)

                          ttCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCttatt                                                                                                       >  1:402530/1‑139 (MQ=255)

                           tCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCttatta                                                                                                      >  2:75350/1‑139 (MQ=255)

                             ccccATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAAc                                                                                                    <  1:75350/139‑1 (MQ=255)

                                  tCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTccc                                                                                               >  1:295696/1‑139 (MQ=255)

                                        gAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCttt                                                                                         >  2:694121/1‑139 (MQ=255)

                                                    tttGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGc                                                                             <  1:694121/139‑1 (MQ=255)

                                                      tGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCgg                                                                                                               <  2:583953/103‑1 (MQ=255)

                                                      tGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCgg                                                                                                               >  1:583953/1‑103 (MQ=255)

                                                        aTTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCAcc                                                                         <  2:356102/139‑1 (MQ=255)

                                                        aTTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCAcc                                                                         >  1:601605/1‑139 (MQ=255)

                                                          ttAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGc                                                                       <  2:1105480/139‑1 (MQ=255)

                                                            aaaTCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCaa                                                                     >  1:995334/1‑139 (MQ=255)

                                                                  caTGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCTTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCaaa                                                               <  1:182705/139‑1 (MQ=255)

                                                                    tGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATa                                                             <  2:601605/139‑1 (MQ=255)

                                                                      ggTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACa                                                           <  2:402530/139‑1 (MQ=255)

                                                                       gTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCgg                                                                                                               <  1:500339/86‑1 (MQ=255)

                                                                       gTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCgg                                                                                                               >  2:500339/1‑86 (MQ=255)

                                                                       gTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCa                                                                      >  2:265194/1‑127 (MQ=255)

                                                                       gTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCa                                                                      <  1:265194/127‑1 (MQ=255)

                                                                       gTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACAt                                                          <  2:995334/139‑1 (MQ=255)

                                                                        tACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACAtt                                                         <  2:295696/139‑1 (MQ=255)

                                                                               aGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGtt                                                                                                                                 >  2:1196701/1‑60 (MQ=255)

                                                                               aGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGtt                                                                                                                                 <  1:1196701/60‑1 (MQ=255)

                                                                                 tGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCgg                                                                                                               >  1:763714/1‑76 (MQ=255)

                                                                                 tGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCgg                                                                                                               <  2:763714/76‑1 (MQ=255)

                                                                                       gTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGccc                                             >  1:502164/1‑136 (MQ=255)

                                                                                       gTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGccc                                             <  2:502164/136‑1 (MQ=255)

                                                                                           gATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACg                                                                              >  2:1157930/1‑99 (MQ=255)

                                                                                           gATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACg                                                                              <  1:1157930/99‑1 (MQ=255)

                                                                                           gATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATa                                      >  2:136953/1‑139 (MQ=255)

                                                                                             tGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAAt                                    >  1:436279/1‑139 (MQ=255)

                                                                                                                    aTCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATAc                                                  >  1:501661/1‑102 (MQ=255)

                                                                                                                    aTCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATAc                                                  <  2:501661/102‑1 (MQ=255)

                                                                                                                    aTCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCa             >  2:309575/1‑139 (MQ=255)

                                                                                                                     tCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCaaa                                                               >  1:883842/1‑88 (MQ=255)

                                                                                                                     tCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCaaa                                                               <  2:883842/88‑1 (MQ=255)

                                                                                                                     tCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCgg                                           <  1:867551/108‑1 (MQ=255)

                                                                                                                     tCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCgg                                           >  2:867551/1‑108 (MQ=255)

                                                                                                                     tCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAg            >  1:259213/1‑139 (MQ=255)

                                                                                                                      cAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATa                                                             <  2:103617/89‑1 (MQ=255)

                                                                                                                      cAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATa                                                             >  1:103617/1‑89 (MQ=255)

                                                                                                                       aaCACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGAc          >  2:1135197/1‑139 (MQ=255)

                                                                                                                       aaCACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGAc          >  1:1007917/1‑139 (MQ=255)

                                                                                                                         cacGCTATGTAATTTGTTCTGGCCCCGACATCTCgg                                                                                                               >  2:294858/1‑36 (MQ=255)

                                                                                                                         cacGCTATGTAATTTGTTCTGGCCCCGACATCTCgg                                                                                                               <  1:294858/36‑1 (MQ=255)

                                                                                                                         cacGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCttt                                                                                 >  2:578744/1‑66 (MQ=255)

                                                                                                                         cacGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCttt                                                                                 <  1:578744/66‑1 (MQ=255)

                                                                                                                         cacGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGc                                                                       <  2:536243/76‑1 (MQ=255)

                                                                                                                         cacGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGc                                                                       >  1:449191/1‑76 (MQ=255)

                                                                                                                         cacGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGc                                                                       >  1:536243/1‑76 (MQ=255)

                                                                                                                         cacGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGc                                                                       <  2:449191/76‑1 (MQ=255)

                                                                                                                         cacGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGAccc        >  2:642615/1‑139 (MQ=255)

                                                                                                                         cacGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGAccc        >  2:685862/1‑139 (MQ=255)

                                                                                                                         cacGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGAccc        >  2:228329/1‑139 (MQ=255)

                                                                                                                            gCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGtt     <  2:436279/139‑1 (MQ=255)

                                                                                                                              tATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCaaa                                                               >  2:587667/1‑79 (MQ=255)

                                                                                                                              tATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCaaa                                                               <  1:587667/79‑1 (MQ=255)

                                                                                                                               aTGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAg  >  2:948225/1‑139 (MQ=255)

                                                                                                                                tGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACATCCCGGTCATa                                      >  2:240755/1‑102 (MQ=255)

                                                                                                                                tGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATa                                      <  1:240755/102‑1 (MQ=255)

TACTTCTTGTGCCAATGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAG  >  NC_000913/1620075‑1620340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A