Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I1 R1	750	51.0	2595496	93.6%	2429384	106.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,620,207	G→A	73 (TAG→TAA)	marB →	periplasmic mar operon regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,620,207	0	G	A	100.0%	105.4 / NA	34	73 (TAG→TAA)	marB	periplasmic mar operon regulator
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (19/15); total (19/15)

GTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCC  >  NC_000913/1620094‑1620334

gTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCggg                                                                                                        >  2:823187/1‑139 (MQ=255)

gTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCggg                                                                                                        >  2:581332/1‑139 (MQ=255)

 tGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCgggg                                                                                                       >  2:596916/1‑139 (MQ=255)

       ttCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCttatt                                                                                                 <  1:80678/139‑1 (MQ=255)

        tCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCggg                                                                                                        <  1:110115/131‑1 (MQ=255)

        tCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCggg                                                                                                        >  2:110115/1‑131 (MQ=255)

                               cGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTAc                                                                         >  2:343417/1‑139 (MQ=255)

                                       ttAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGc                                                                 <  2:33679/139‑1 (MQ=255)

                                                       cTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATa                                                       >  1:1295362/1‑133 (MQ=255)

                                                       cTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATa                                                       <  2:1295362/133‑1 (MQ=255)

                                                        tGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCaaa                                                         <  2:355166/130‑1 (MQ=255)

                                                        tGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCaaa                                                         >  1:355166/1‑130 (MQ=255)

                                                         ggCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGata                                               <  1:581332/139‑1 (MQ=255)

                                                                        gATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATa                                >  1:276720/1‑139 (MQ=255)

                                                                               tCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGcc                                                            <  1:355808/104‑1 (MQ=255)

                                                                               tCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGcc                                                            >  2:355808/1‑104 (MQ=255)

                                                                                 ggCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTTATAATGAgcacc                       <  1:596916/139‑1 (MQ=255)

                                                                                                 aTCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATa                                                       <  2:1019779/91‑1 (MQ=255)

                                                                                                 aTCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATa                                                       >  1:1019779/1‑91 (MQ=255)

                                                                                                  tCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATa                                                       <  1:1013679/90‑1 (MQ=255)

                                                                                                  tCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATa                                                       >  2:1013679/1‑90 (MQ=255)

                                                                                                  tCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAg      >  1:701734/1‑139 (MQ=255)

                                                                                                    aaCACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGcc                                        >  1:178752/1‑103 (MQ=255)

                                                                                                    aaCACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGcc                                        <  2:178752/103‑1 (MQ=255)

                                                                                                      cacGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTTACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATa                                                       <  2:1109332/86‑1 (MQ=255)

                                                                                                      cacGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATa                                                       <  1:836537/86‑1 (MQ=255)

                                                                                                      cacGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATa                                                       >  1:1109332/1‑86 (MQ=255)

                                                                                                      cacGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACa                                                     <  2:1181772/88‑1 (MQ=255)

                                                                                                      cacGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACa                                                     >  1:1181772/1‑88 (MQ=255)

                                                                                                      cacGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGAccc  >  1:324792/1‑139 (MQ=255)

                                                                                                      cacGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGAccc  >  1:180733/1‑139 (MQ=255)

                                                                                                      cacGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCACTTTACGCCACCGCAAGCCAAATa                                                       >  2:836537/1‑86 (MQ=255)

                                                                                                             tGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCa                                                                <  2:961072/70‑1 (MQ=255)

                                                                                                             tGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCa                                                                >  1:961072/1‑70 (MQ=255)

GTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCC  >  NC_000913/1620094‑1620334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A