Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I2 R1	762	35.5	1545288	95.5%	1475750	117.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,620,207	G→A	73 (TAG→TAA)	marB →	periplasmic mar operon regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,620,207	0	G	A	100.0%	133.4 / NA	42	73 (TAG→TAA)	marB	periplasmic mar operon regulator
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/20); total (22/20)

CTTCTTGTGCCAATGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGC  >  NC_000913/1620077‑1620341

cttcttGTGCCAATGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCt                                                                                                                                <  1:438446/139‑1 (MQ=255)

cttcttGTGCCAATGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCt                                                                                                                                >  2:708594/1‑139 (MQ=255)

      gtgCCAATGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGcccc                                                                                                                          >  2:563849/1‑139 (MQ=255)

                 gTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCggg                                                                                                               >  2:632028/1‑139 (MQ=255)

                 gTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCggg                                                                                                               <  1:708594/139‑1 (MQ=255)

                       gttCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCttat                                                                                                         >  1:363376/1‑139 (MQ=255)

                            cccATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACt                                                                                                    <  1:707788/139‑1 (MQ=255)

                                  gCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCAc                                                                                              <  1:563849/139‑1 (MQ=255)

                                           cccaccGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGc                                                                                     >  1:561937/1‑139 (MQ=255)

                                                  tttGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGc                                                                              <  1:373620/139‑1 (MQ=255)

                                                                  tGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATa                                                              <  1:694549/139‑1 (MQ=255)

                                                                  tGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATa                                                              <  2:364018/139‑1 (MQ=255)

                                                                  tGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATa                                                              <  1:632028/139‑1 (MQ=255)

                                                                        cTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGa                                                        >  2:675923/1‑139 (MQ=255)

                                                                         tGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCaa                                                                      <  2:209224/124‑1 (MQ=255)

                                                                         tGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCaa                                                                      >  1:209224/1‑124 (MQ=255)

                                                                           gCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTGTAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGatat                                                     >  2:216560/1‑139 (MQ=255)

                                                                               tGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAg                                                 >  2:313330/1‑139 (MQ=255)

                                                                               tGATAAGTCGGATGCGCTCGGCGTGCCCAATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGc                                                                        <  1:617935/116‑1 (MQ=255)

                                                                               tGATAAGTCGGATGCGCTCGGCGTGCCCAATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGc                                                                        >  2:617935/1‑116 (MQ=255)

                                                                                 aTAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGcc                                               <  2:395345/139‑1 (MQ=255)

                                                                                           tGCGCTCGGCGTGCCCTATTGTAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAAt                                     <  1:216560/139‑1 (MQ=255)

                                                                                           tGCGCTCGGCGTGCCCTATTATAATCATCACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAAt                                     >  2:63112/1‑139 (MQ=255)

                                                                                               cTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGatat                                                     <  2:176399/119‑1 (MQ=255)

                                                                                               cTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGatat                                                     >  1:176399/1‑119 (MQ=255)

                                                                                                 cGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAgcac                               <  2:688384/139‑1 (MQ=255)

                                                                                                      tGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAgcaccgcac                          <  1:675923/139‑1 (MQ=255)

                                                                                                                  aTCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCa              >  1:239136/1‑139 (MQ=255)

                                                                                                                     aaCACGCTATGTAATTTGTTCTGGCCCCGACATCTCgg                                                                                                                <  2:89466/38‑1 (MQ=255)

                                                                                                                     aaCACGCTATGTAATTTGTTCTGGCCCCGACATCTCgg                                                                                                                >  1:89466/1‑38 (MQ=255)

                                                                                                                     aaCACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCt                                                                                    >  2:581620/1‑66 (MQ=255)

                                                                                                                     aaCACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCt                                                                                    <  1:581620/66‑1 (MQ=255)

                                                                                                                     aaCACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATa                                                              <  2:537308/88‑1 (MQ=255)

                                                                                                                     aaCACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATa                                                              >  1:537308/1‑88 (MQ=255)

                                                                                                                       cacGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTccc                                                                                                >  2:541340/1‑52 (MQ=255)

                                                                                                                       cacGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTccc                                                                                                <  1:541340/52‑1 (MQ=255)

                                                                                                                       cacGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGAccc         >  2:196429/1‑139 (MQ=255)

                                                                                                                       cacGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGAccc         >  1:245031/1‑139 (MQ=255)

                                                                                                                       cacGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACC‑TTACCGCTTTACGCCACCGCAAGCCAAATa                                                              <  2:139508/85‑1 (MQ=255)

                                                                                                                       cacGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACC‑TTACCGCTTTACGCCACCGCAAGCCAAATa                                                              >  1:139508/1‑85 (MQ=255)

                                                                                                                          gCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGtt      >  2:13731/1‑139 (MQ=255)

                                                                                                                              tGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGc  >  1:225844/1‑139 (MQ=255)

CTTCTTGTGCCAATGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGC  >  NC_000913/1620077‑1620341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A